Anti-inflammatory effect of Garcinia mangostana Linn. pericarp extract in methicillin-resistant Staphylococcus aureus-induced superficial skin infection in mice

Tatiya-aphiradee, Nitima; Chatuphonprasert, Waranya; Jarukamjorn, Kanokwan

doi:10.1016/j.biopha.2018.12.142

Cited by 42 publications

(31 citation statements)

References 51 publications

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“…an abrasive wound in S. aureus skin-infected patients (10,14). The TEWL indicates skin barrier damage (10) and it was used to normalize the same level of skin barrier damage in all mice on the first day of study. After the tape stripping, the application of MRSA inoculum to an abrasive wound allowed MRSA to grow, permeate the wound, and stimulate the systematic inflammatory response.…”

Section: Discussionmentioning

confidence: 99%

“…After the tape stripping, the application of MRSA inoculum to an abrasive wound allowed MRSA to grow, permeate the wound, and stimulate the systematic inflammatory response. Fibrin deposition in the skin is an eminent and compatible marker for type IV hypersensitivity skin reactions, contact dermatitis, and MRSA-infected skin (10,15). Neutrophil infiltration is a process required for an effective immune response against S. aureus (16).…”

Section: Discussionmentioning

confidence: 99%

“…Swabs from infected-wounds were cultured on 6 µg/mL oxacillin-contained MSA plates on days 2, 4, 6, and 8. The number of MRSA colonies was recorded after incubation for 18 -24 hours (10). Wounds were photographed on days 1, 3, 5, 7, and 9.…”

Section: Tape-stripping Mouse Modelmentioning

confidence: 99%

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Antibacterial and Wound Healing Activity of Dipterocarpus alatus Crude Extract Against Methicillin-resistant Staphylococcus aureus-induced Superficial Skin Infection in Mice

et al. 2019

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Background: The bark oil of Dipterocarpus alatus Roxb. ex G. Don (resin tree) has been used for the treatment of ulcerated wounds in Ayurvedic medicine. However, information is still scarce about D. alatus extract and its constituents. Objectives: This study aimed to examine the antibacterial and wound healing effects of D. alatus crude extracts against methicillinresistant Staphylococcus aureus (MRSA) in a superficial skin infection using a tape-stripping mouse model. Methods: Different parts (bark, leaves, twig, wood, and oleo-resin) of D. alatus were used for extraction. Crude extracts with two pure compounds (α,β-gurjunene and dipterocarpol) were examined for antibacterial activity against methicillin-susceptible S. aureus (MSSA) and MRSA in vitro. The in vivo antibacterial activity against MRSA was examined in a tape-stripping mouse model of superficial skin infection by evaluating transepidermal water loss (TEWL), colony counts from wound culture swabs, and H&E staining. Results: Dipterocarpus alatus twig extract showed the lowest MIC (250 µg/mL) and MBC (500 µg/mL) against MRSA among extracts from other D. alatus parts. In addition, α,β-gurjunene showed lower MIC (250 µg/mL) and MBC (250 µg/mL) against MRSA than dipterocarpol. In vivo, D. alatus twig extract significantly reduced the number of MRSA recovered from superficial wounds to levels comparable to the non-infected control group on the second day. Oleo-resin, D. alatus wood extract, α,β-gurjunene, and dipterocarpol also significantly reduced the number of MRSA. Moreover, D. alatus twig extract and dipterocarpol healed infected wounds at rates comparable to the non-infected control group. From H&E staining of wounds, fibrin accumulation and neutrophil infiltration were attenuated in mice treated with D. alatus twig extract and dipterocarpol. Conclusions: Dipterocarpus alatus twig extract and dipterocarpol possess antibacterial and wound healing properties against MRSAinduced superficial skin infection in mice.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Antibacterial and Wound Healing Activity of Dipterocarpus alatus Crude Extract Against Methicillin-resistant Staphylococcus aureus-induced Superficial Skin Infection in Mice

et al. 2019

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“…MIC was determined using a serial-dilution method in Mueller Hinton broth (MHB) as previously reported. 15 Inocula (2 × 01 6 CFU/mL of either MSSA DMST-2933 or MRSA DMST-20651) were prepared by resuspending isolated bacterial colonies in sterile 0.9% NaCl and adjusting the turbidity to match a McFarland standard solution. The inocula were incubated in MHB and serial-dilutions of each of the D. alatus extracts (0.195 -100 mg/mL), or diptrocarpol (0.125 -32 µg/mL), or tetracycline (0.125 -32 µg/mL) at 37C for 18-24 h. The live bacteria were quantified by the degree of resazurin-colour change compared to control medium without bacterial inoculum.…”

Section: Determination Of Minimum Inhibitory Concentration (Mic) and mentioning

confidence: 99%

“…For MBC determination, all the media in the wells with a higher concentration than the MIC value were pipetted and spread on Mueller Hinton agar, then incubated at 37C for 18-24 h. The lowest concentration of each of the test compounds with no colonies of MSSA or MRSA was the MBC. 15 MIC and MBC were determined from two-independent experiments (n = 4-5).…”

Section: Determination Of Minimum Inhibitory Concentration (Mic) and mentioning

confidence: 99%

Antibacterial Activity of Dipterocarpus alatus Twig Extract against Methicillin-Resistant Staphylococcus aureus (MRSA)

2020

TJNPR

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Dipterocarpus alatus has been used for the treatment of external wounds and skin diseases in traditional medicine. Staphylococcus aureus is a major pathogen for human superficial skin infections. This study determined the antibacterial effects of D. alatus twig crude extracts against methicillin-sensitive/resistant S. aureus (MSSA and MRSA) in vitro and in vivo. Several organic solvents were used to extract D. alatus twig. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of the D. alatus extracts for MSSA and MRSA were investigated using broth micro-dilution assays. The most potent extract was studied in a mouse superficial wound model with MRSA infection. The ethyl acetate-methanol extract of D. alatus, MeOH(EtOAc), showed the lowest MIC and MBC values (0.390 and 0.781 mg/mL, respectively) against both MSSA and MRSA compared to the other extracts. MRSAinfected mouse superficial wounds showed increased transepidermal water loss (TEWL), mast cell accumulation, and MRSA colony numbers. Treatment with MeOH(EtOAc) extract (20 mg/mL), or MeOH(EtOAc) emulgel (20-40 mg/mL), 100 µL applied daily for 10 days, reduced TEWL values, mast cell accumulation, and MRSA colony numbers in wounds compared to the non-treated group. The wounds of the MeOH(EtOAc) extract and MeOH(EtOAc) emulgel treatment groups were healed on day 8 while the wounds of the dipterocarpol and tetracycline treatment groups were not. Therefore, MeOH(EtOAc) extract or MeOH(EtOAc) emulgel are good candidates for a topical product to treat ulcerated wounds with MRSA infection.

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Anti‐inflammatory Agents from Medicinal Plants

Tùng

Linh

Thao

et al. 2023

Phytochemical Drug Discovery for Central Nervous System Disorders

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Anti-inflammatory effect of Garcinia mangostana Linn. pericarp extract in methicillin-resistant Staphylococcus aureus-induced superficial skin infection in mice

Cited by 42 publications

References 51 publications

Antibacterial and Wound Healing Activity of Dipterocarpus alatus Crude Extract Against Methicillin-resistant Staphylococcus aureus-induced Superficial Skin Infection in Mice

Antibacterial and Wound Healing Activity of Dipterocarpus alatus Crude Extract Against Methicillin-resistant Staphylococcus aureus-induced Superficial Skin Infection in Mice

Antibacterial Activity of Dipterocarpus alatus Twig Extract against Methicillin-Resistant Staphylococcus aureus (MRSA)

Anti‐inflammatory Agents from Medicinal Plants

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