Anti-inflammatory effect of gamma-irradiated genistein through inhibition of NF-κB and MAPK signaling pathway in lipopolysaccharide-induced macrophages

Byun, Eui‐Baek; Sung, Nak-Yun; Yang, Miso; Lee, Byung-Soo; Song, Du-Sup; Park, Jan-Nam; Kim, Jae‐Hun; Jang, Beom‐Su; Choi, DaeSeong; Park, Sang‐Hyun; Yu, Young-Beob; Byun, Eui-Hong

doi:10.1016/j.fct.2014.08.019

Cited by 21 publications

(14 citation statements)

References 38 publications

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“…Therefore, the noticed decrease of LPS-triggered NO production caused by extracts is due to their capacity to limit Nos2 gene transcription, hypothetically by interfering with pro-inflammatory transcription factors such as nuclear factor-κB (NF-κB) and signal transducer and activator of transcription 1 (STAT-1). In fact, the flavonoids genistein, prunetin, biochanin A derivatives, and kampferol have been shown to inhibit NO production by decreasing the activation of NF-κB signaling pathway [15,16,[27][28][29]. When analyzing the effects over Ptgs2 transcription it was found that significant decreases are only obtained by treatment with extracts from roots ( Figure 4).…”

Section: [μG/ml] Figmentioning

confidence: 99%

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Flavonoid Profile of the Genista tridentata L., a Species Used Traditionally to Treat Inflammatory Processes

et al. 2020

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Ethnopharmacological surveys on Portuguese flora reveal that Genista tridentata L. is a shrub used in traditional medicine for the treatment of various inflammation-related health problems, although scientific support of its benefits is still necessary. In order to establish the anti-inflammatory potential of G. tridentata and support its traditional use, ethanolic extracts of three sections of the plant (root, stem, and leaves) were subjected to in vitro evaluation of anti-inflammatory activity using lipopolysaccharide (LPS)-stimulates macrophages as an inflammation model. Simultaneously, we also aimed to establish the extracts’ flavonoids profile. The ethanolic extracts, obtained by Soxhlet extraction, profile of the three sections confirmed their richness in flavonoids, being three prenylated flavonoids isolated and characterized in the root, including a new natural compound, the 3-methoxymundulin. The extracts from the three plant sections showed strong antioxidant activity at the cellular level and significantly inhibit the LPS-triggered NO production by downregulating Nos2 gene transcription and consequently iNOS expression. Additionally, root and stem extracts also decreased the LPS-induced transcription of the pro-inflammatory genes Il1b, Il6, and Ptgs2. Thus, the results support the anti-inflammatory properties attributed to G. tridentate preparations. Relevantly, the roots of the shrub, plant part not used, is an unexplored source of compounds with pharmacological and nutraceutical value.

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Section: [μG/ml] Figmentioning

confidence: 99%

“…The above-mentioned works deal with the aerial parts or flowers and are responsible for the isolation and identification of six isoflavones and three flavone derivatives, from which genistein, prunetin, rutin, and isoquercitrin are due to some already established biological properties [6,7,[14][15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Flavonoid Profile of the Genista tridentata L., a Species Used Traditionally to Treat Inflammatory Processes

et al. 2020

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“…Phytoestrogens may also modulate the phenotype of immune cells in the gut. Isoflavones and lignans suppress macrophage/monocyte activity by inhibiting reactive oxygen species release, cyclooxygenase-2 and NF-κB activation, and proinflammatory cytokine production after LPS stimulation in vitro [139,[143][144][145][146]. DCs express ERs, and therefore their behavior may also be modulated by phytoestrogens [147].…”

Section: Phytoestrogen Metabolismmentioning

confidence: 99%

The “Gut Feeling”: Breaking Down the Role of Gut Microbiome in Multiple Sclerosis

2018

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Multiple sclerosis (MS) is a chronic neuroinflammatory disease of the central nervous system with unknown etiology. Recently, the gut microbiota has emerged as a potential factor in the development of MS, with a number of studies having shown that patients with MS exhibit gut dysbiosis. The gut microbiota helps the host remain healthy by regulating various functions, including food metabolism, energy homeostasis, maintenance of the intestinal barrier, inhibition of colonization by pathogenic organisms, and shaping of both mucosal and systemic immune responses. Alteration of the gut microbiota, and subsequent changes in its metabolic network that perturb this homeostasis, may lead to intestinal and systemic disorders such as MS. Here we discuss the findings of recent MS microbiome studies and potential mechanisms through which gut microbiota can predispose to, or protect against, MS. These findings highlight the need of an improved understanding of the interactions between the microbiota and host for developing therapies based on gut commensals with which to treat MS.

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“…Genistein, a kind of natural phytoestrogens from soybeans, can be absorbed directly by intestinal epithelial cells through passive diffusion ( Soukup et al., 2016 ). It is suggested that genistein inhibits the activity of tyrosine kinase and mitogen activated protein kinase ( MAPK )/NF-κB signalling pathway, which play important roles in anti-inflammatory effects ( Markovits et al., 1989 , Byun et al., 2014 ). Similarly, a previous study indicated that genistein can inhibit NF-κB activation and IL-17 secretion in CD4+ T cells, which relieve the inflammation reaction ( Kehlen et al., 1999 ).…”

Section: Introductionmentioning

confidence: 99%

Dietary genistein supplementation protects against lipopolysaccharide-induced intestinal injury through altering transcriptomic profile

Dai

Song

et al. 2020

Poultry Science

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Genistein is abundant in the corn-soybean meal feed. Little information is available about the effect of dietary genistein on the intestinal transcriptome of chicks, especially when suffering from intestinal injury. In this study, 180 one-day-old male ROSS 308 broiler chickens were randomly allocated to 3 groups, with 4 replicates (cages) of 15 birds each. The treatments were as follows: chicks received a basal diet (CON), a basal diet and underwent lipopolysaccharide-challenge (LPS), or a basal diet supplemented with 40 mg/kg genistein and underwent LPS-challenge (GEN). LPS injection induced intestinal injury and inflammatory reactions in the chicks. Transcriptomic analysis identified 7,131 differently expressed genes (3,281 upregulated and 3,851 downregulated) in the GEN group compared with the LPS group ( P adjusted value < 0.05, |fold change| > 1.5), which revealed that dietary genistein exposure altered the gene expression profile and signaling pathways in the ileum of LPS-treated chicks. Furthermore, dietary genistein improved intestinal morphology, mucosal immune function, tight junction, antioxidant activity, apoptotic process, and growth performance, which were adversely damaged by LPS injection. Therefore, adding genistein into the diet of chicks can alter RNA expression profile and ameliorate intestinal injury in LPS-challenged chicks, thereby improving the growth performance of chicks with intestinal injury.

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Anti-inflammatory effect of gamma-irradiated genistein through inhibition of NF-κB and MAPK signaling pathway in lipopolysaccharide-induced macrophages

Cited by 21 publications

References 38 publications

Flavonoid Profile of the Genista tridentata L., a Species Used Traditionally to Treat Inflammatory Processes

Flavonoid Profile of the Genista tridentata L., a Species Used Traditionally to Treat Inflammatory Processes

The “Gut Feeling”: Breaking Down the Role of Gut Microbiome in Multiple Sclerosis

Dietary genistein supplementation protects against lipopolysaccharide-induced intestinal injury through altering transcriptomic profile

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