Anti-inflammatory effect of fucoidan isolated from fermented Sargassum fusiforme in in vitro and in vivo models

Wang, Lei; Cui, Yong Ri; Wang, Kaiqiang; Fu, Xiaoting; Xu, Jiachao; Gao, Xin; Jeon, You‐Jin

doi:10.1016/j.ijbiomac.2022.10.005

Cited by 19 publications

(7 citation statements)

References 29 publications

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“…Meanwhile, at 50 μg/mL, they showed 88%, 61%, 60% and 57% reductions in iNOS mRNA expression, respectively, compared to the control group, while the highest reduction was observed at 100 μg/mL, showing 92%, 68%, 65% and 63% with respect to the control group, where MIF1 showed the highest inhibitory activity at all tested doses. This is supported by an investigation using fucoidan extracted from Sargassum fusiforme, where the results demonstrated the inhibition of NO production and PGE 2 by down-regulating iNOS and COX-2 [ 50 ]. Furthermore, a fucose-rich polysaccharide fraction of Chnoospora minima also decreased the production of PGE 2 , TNF-α, IL−1β, IL-6, NO, iNOS, and COX-2 expression in a dose-dependent manner.…”

Section: Resultsmentioning

confidence: 67%

FTIR Characterization of Sulfated Polysaccharides Obtained from Macrocystis integrifolia Algae and Verification of Their Antiangiogenic and Immunomodulatory Potency In Vitro and In Vivo

et al. 2022

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The aim of this study was to evaluate the antiangiogenic and immunomodulatory potential of sulfated polysaccharides from the marine algae Macrocystis integrifolia characterized by FTIR. The cytotoxicity of sulfated polysaccharides was evaluated using the 3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide (MTT) assay. Antiangiogenic activity was evaluated using the chicken chorioallantoic membrane (CAM) assay. Immunomodulatory activity was determined on macrophage functionality and allergic response. The results showed that sulfated polysaccharides significantly decreased angiogenesis in chicken chorioallantoic membranes (p < 0.05). Likewise, they inhibited in vivo chemotaxis and in vitro phagocytosis, the transcription process of genes that code the enzymes cyclooxygenase−1 (COX−1), cyclooxygenase-2 (COX-2) and nitric oxide synthase-2 (NOS-2) and the nuclear factor kappa-light chain enhancer of activated B cells (NF-κB), showing immunomodulatory properties on the allergic response, as well as an in vivo inhibitory effect in the ovalbumin-induced inflammatory allergy model (OVA) and inhibited lymphocyte proliferation specific to the OVA antigen in immunized mice. Finally, these compounds inhibited the histamine-induced skin reaction in rats, the production of immunoglobulin E (IgE) in mice, and the passive response to skin anaphylaxis in rats. Therefore, the results of this research showed the potential of these compounds to be a promising source for the development of antiangiogenic and immunomodulatory drugs.

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Section: Resultsmentioning

confidence: 67%

FTIR Characterization of Sulfated Polysaccharides Obtained from Macrocystis integrifolia Algae and Verification of Their Antiangiogenic and Immunomodulatory Potency In Vitro and In Vivo

et al. 2022

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“…RAW 264.7 cells (2 × 10 6 cells/well) were pretreated with each sample for the screening test (500 and 1000 μg/mL) or F-CSA (125–1000 μg/mL) for 1 h, after which they were stimulated with LPS (1 μg/mL) for 24 h. Nitrite accumulation in the culture medium as an indicator of nitric oxide (NO) production was measured using the Griess reagent [ 32 ]. The cell supernatant was mixed 1:1 with 100 μL of Griess reagent for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Fermented Sprouts of Codonopsis lanceolata Suppress LPS-Induced Inflammatory Responses by Inhibiting NF-κB Signaling Pathway in RAW 264.7 Macrophages and CD1 Mice

Choi

Kim

et al. 2023

Pharmaceutics

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The interest in bioconversion through fermentation of sprouts produced in smart farms is increasing due to their potential health benefits. Codonopsis lanceolata (CL) is reported to alleviate inflammatory conditions, but much research is still needed to determine which types and parts of CL are most effective. This study investigated the anti-inflammatory effects of a fermented extract of CL sprouts’ aerial part (F-CSA) against LPS-stimulated RAW 264.7 macrophages and mice. In the screening test, F-CSA showed the most substantial anti-inflammatory effect among several samples, containing the highest total flavonoids, tannins, and polyphenols. UPLC-ESI-Q/TOF-MS and HPLC analysis revealed that F-CSA had the highest amount of luteolin among all the CL samples analyzed. F-CSA reduced the release of inflammatory cytokines and mediators such as NO and PGE2 by inhibiting the expression levels of iNOS and COX-2 in LPS-stimulated macrophages. Further, we found that the anti-inflammatory effects of F-CSA were mediated by inhibiting the JNK/NF-κB signaling pathway. Moreover, F-CSA improved survival rates and reduced plasma levels of NO and IL-6 in CD1 mice stimulated with LPS. These findings suggest that F-CSA, which contains luteolin, can alleviate inflammation in LPS-induced RAW 264.7 cells and a CD1 mouse model by inhibiting the JNK/NF-κB signaling pathways.

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“…Likewise, different studies have shown that fucoidans modulate inflammatory responses thought inhibition of NF-κB [ 33 , 67 , 70 ]. For instance, fucoidans isolated using Viscozyme-assisted enzymatic extraction of Sargassum coreanum and showing high sulfate and fucose contents, and fucoidans from fermented Sargassum fusiforme obtained by ethanol precipitation, suppressed pro-inflammatory cytokine production (i.e., TNF-α, IL-1β, and IL-6) and NO by modulating NF-κB signaling in LPS-induced RAW 264.7 macrophages cells [ 71 , 72 ]. Similarly, a recent study demonstrated that fucoidans from Undaria pinnatifida, Fucus vesiculosus , and Macrocystis pyrifera can inhibit inflammatory responses and protect against mitochondrial dysfunction in cultured osteoarthritis chondrocytes by attenuating NF-κB nuclear translocation [ 73 ].…”

Section: Mechanisms Of Fucoidan Actionsmentioning

confidence: 99%

Relevance of the Extraction Stage on the Anti-Inflammatory Action of Fucoidans

et al. 2023

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The anti-inflammatory action of fucoidans is well known, based on both in vitro and some in vivo studies. The other biological properties of these compounds, their lack of toxicity, and the possibility of obtaining them from a widely distributed and renewable source, makes them attractive novel bioactives. However, fucoidans’ heterogeneity and variability in composition, structure, and properties depending on seaweed species, biotic and abiotic factors and processing conditions, especially during extraction and purification stages, make it difficult for standardization. A review of the available technologies, including those based on intensification strategies, and their influence on fucoidan composition, structure, and anti-inflammatory potential of crude extracts and fractions is presented.

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Anti-inflammatory effect of fucoidan isolated from fermented Sargassum fusiforme in in vitro and in vivo models

Cited by 19 publications

References 29 publications

FTIR Characterization of Sulfated Polysaccharides Obtained from Macrocystis integrifolia Algae and Verification of Their Antiangiogenic and Immunomodulatory Potency In Vitro and In Vivo

FTIR Characterization of Sulfated Polysaccharides Obtained from Macrocystis integrifolia Algae and Verification of Their Antiangiogenic and Immunomodulatory Potency In Vitro and In Vivo

Fermented Sprouts of Codonopsis lanceolata Suppress LPS-Induced Inflammatory Responses by Inhibiting NF-κB Signaling Pathway in RAW 264.7 Macrophages and CD1 Mice

Relevance of the Extraction Stage on the Anti-Inflammatory Action of Fucoidans

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