Anti‐Inflammatory Effect of Feiyangchangweiyan Capsule on Rat Pelvic Inflammatory Disease through JNK/NF‐<i>κ</i>B Pathway

Li, Yao; Liu, Yang; Yang, Qian; Shi, Zhihui; Xie, Youhua; Wang, Siwang

doi:10.1155/2018/8476147

Cited by 3 publications

(3 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Three specimens were mounted on each glass slide. Inflammatory cell count was performed by counting the mean number of macrophage cells, neutrophils, as well as uterine and ovarian lymphocytes [24] using a 40 jar magnification and 40x objective microscope. Inflammatory cells are considered identified if infiltration of inflammatory cells, including lymphocytes and neutrophils, was observed.…”

Section: Methodsmentioning

confidence: 99%

The Effect and Activity of Free Radical Enzymes Due to Arsenic Exposure Through the Vulva and Vagina

Irnawati

Idroes

Akmal

et al. 2022

Open Access Maced J Med Sci

View full text Add to dashboard Cite

BACKGROUND OF THE STUDY: Geogenic arsenic is ubiquitous, found in water and soil that is used daily, can be exposed to the female body through the genital organs. The vulva and vagina are open channels that allow toxic agents to enter the internal genitalia and distributed throughout the body. AIM OF THE STUDY: This study investigated the effects of vaginal arsenic exposure via vulvar immersion and vaginal douching in Rattus norvegicus on the damage of uterus and ovaries through oxidative mechanisms (MDA, SOD, and H₂O₂). METHODOLOGY: The experimental animals were divided into three treatment groups, i.e., K0 (control group), K1 (group treated with vulvar immersion in 0.8 mg/L arsenic solution), and K2 (group treated with vaginal douching using 0.5-mL of 0.8 mg/L arsenic solution). For each group, the treatment was repeated six times and carried out for fourteen days. Before the study, a seven-day acclimatization period was conducted for adaptation purposes. The experimental animals were euthanized using ketamine xylazine. The uterus and ovaries were collected for MDA, SOD, and H₂O analysis, as well as histopathology examination. RESULTS: The vaginal douching group had the highest MDA level both on the uterus (210.66±4.92μM) and the ovaries (214.67±2.50 μM). The immersion group also experienced an increase in MDA in the uterus (198.66±3.33μM) and ovaries (206.33± .21μM). However, a higher level of MDA was found in the ovaries. The highest H₂O₂ level was also found in the uterine and ovarian organs in the douching group. In contrast, the lowest SOD levels of uterine and ovarian were identified in the vaginal douching group. Arsenic exposure through vaginal immersion and douching affected the uterine MDA, SOD, and H₂O levels (Ρ<0.05). Arsenic exposure through vaginal douching also affected the ovarian MDA, SOD, and H₂O levels (Ρ<0.05). There was a significant difference in the mean of inflammatory cells (infiltrated neutrophils, macrophages, and lymphocytes) in the uterus and ovaries in the control, immersion, and vaginal douching groups (Ρ<0.05). CONCLUSION: Exposure to 0.8 mg/L arsenic solution through vulvar immersion and vaginal douching can cause oxidative stress and trigger inflammation of the uterine and ovarian tissue.

show abstract

Section: Methodsmentioning

confidence: 99%

The Effect and Activity of Free Radical Enzymes Due to Arsenic Exposure Through the Vulva and Vagina

Irnawati

Idroes

Akmal

et al. 2022

Open Access Maced J Med Sci

View full text Add to dashboard Cite

show abstract

“…The number of inflammatory cells was obtained from the average number of macrophages, neutrophils, and lymphocytes. 37 To reduce bias in the study, two pathologists did the observation.…”

Section: Uterus Histopathologymentioning

confidence: 99%

Effect of arsenic exposure on MDA, SOD, H2O2 and TNF-α levels of uterus homogenate of female Sparague-Dawleys rats

Irnawati¹,

Idroes

Akmal

et al. 2023

F1000Res

View full text Add to dashboard Cite

Background and Aim: Arsenic exposure to the body through the oral, dermal, and inhalation routes have a detrimental impact on health, including women’s reproductive health. However, the effect of arsenic exposure through the vulva of women is unclear. The present study therefore examined the effects of long-term arsenic exposure of vulva on uterus inflammation mediated by oxidative stress and inflammatory mechanism. Materials and Methods: Female Rattus norvegicus L was used as the animal model and the arsenic were exposed through vulvar immersion. The arsenic solution was made into four concentrations while the duration of exposure was made in four-time combinations. Uterus inflammation was assessed through histopathological observation of uterus tissue through hematoxylin and eosin (H&E) staining. Oxidative stress was assessed using malondialdehyde (MDA), superoxide dismutase (SOD), and hydrogen peroxide (H2O2); while inflammatory profile was assessed by measuring using tumor necrosis factor α (TNFα). Results: Our data suggested that inflammation could occur at the permissible quality standard concentrations when arsenic was exposed for more than two weeks. At higher concentrations and a longer exposure time, arsenic exposure could lead to chronic inflammation. Arsenic exposure was able to increase the levels of MDA and H2O2 and reduced the SOD suggesting stress oxidative of the organ. Arsenic also could increase the level of TNFα at any concentration after 6 and 8 weeks of exposure suggesting the inflammation process in the uterus. Conclusion: Continuous exposure of vulva with arsenic could induce oxidative stress and chronic inflammation of the uterus. Further study to investigate this finding in human is critical as basic to propose health campaign program to the community in the high arsenic regions.

show abstract

“…The laboratory study confirms that Cortex phellodendri and Humulus japonicus exert good therapeutic effect on mice with chronic PID by inhibiting the expression of inflammatory cytokines and neutrophil infiltration [ 13 ]. Furthermore, Feiyangchangweiyan is reported to suppress infiltration and apoptosis of inflammatory cells in uterine tissues by preventing NF-κB nuclear translocation in pathogen-induced PID rats [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Metabonomic study of the protective effect of Fukeqianjin formula on multi-pathogen induced pelvic inflammatory disease in rats

Zhang

Zou

et al. 2018

Chin Med

View full text Add to dashboard Cite

BackgroundFukeqianjin formula has been effectively used in the treatment of pelvic inflammatory disease (PID) and the related complications in clinic. Although there have been some studies about the underlying mechanism that focus on its anti-inflammatory and immunoregulatory activities. But the mechanism is still not fully understood. The aim of this study was to investigate the alteration of plasma metabolic profiles in PID rats and the regulatory effect of Fukeqianjin formula on potential biomarkers.MethodsPelvic inflammatory model was established by intrauterine inoculation of multiple pathogens combined with mechanical injury of endometrium. Rats were randomly divided into normal group, model group, azithromycin group, high-and low-dose of Fukeqianjin formula treatment group (FF-H, and FF-L, respectively). After 14 days of intragastric administration, the plasm levels of interleukin-1β (IL-1β) and nitric oxide (NO) were measured. To further recognize and identify potential biomarkers and metabolic pathways, an ultra-performance liquid chromatography-quadrupole-Exactive Orbitrap-mass spectrometry (UPLC-Q-Exactive Orbitrap-MS) metabonomic method combined with multivariate analyses including principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) and orthogonal partial least squares discriminant analysis (OPLS-DA), was employed to analyze the metabolic profiling.ResultsCompared with normal group, the plasma levels of IL-1β and NO were significantly increased in the PID model group (P < 0. 05), and obviously decreased after high-dose intervention of Fukeqianjin formula (P < 0. 01). The PCA, PLS-DA and OPLS-DA analysis showed that PID rats were clearly separated from normal rats. Compared with the PID model group, the metabolite profiles of Fukeqianjin formula treatment group was gradually restored to normal. Meanwhile, 14 potential metabolite biomarkers, which were mainly related to the metabolic pathways of intervening glycerophospholipid metabolism, linoleic acid metabolism/alpha-linolenic acid metabolism, amino acid metabolism, arachidonic acid metabolism, and unsaturated fatty acids biosynthesis, have been identified. Fukeqianjin formula exerts good regulatory effect on the abnormal metabolism of PID rats.ConclusionsIntrauterine inoculation of multiple pathogens combined with mechanical injury of endometrium could significantly disturb the plasma metabolic profiles of rats. Fukeqianjin formula has potential therapeutic effect on multi-pathogen-induced PID by ameliorating metabolism disorders and alleviating the inflammatory response.Electronic supplementary materialThe online version of this article (10.1186/s13020-018-0217-6) contains supplementary material, which is available to authorized users.

show abstract

Anti‐Inflammatory Effect of Feiyangchangweiyan Capsule on Rat Pelvic Inflammatory Disease through JNK/NF‐κB Pathway

Cited by 3 publications

References 45 publications

The Effect and Activity of Free Radical Enzymes Due to Arsenic Exposure Through the Vulva and Vagina

The Effect and Activity of Free Radical Enzymes Due to Arsenic Exposure Through the Vulva and Vagina

Effect of arsenic exposure on MDA, SOD, H2O2 and TNF-α levels of uterus homogenate of female Sparague-Dawleys rats

Metabonomic study of the protective effect of Fukeqianjin formula on multi-pathogen induced pelvic inflammatory disease in rats

Contact Info

Product

Resources

About