Anti-inflammatory effect of dialysable leucocyte extract in a rat model of osteoarthritis: histopathological and molecular characterization

Acosta, P.; Pérez, Netzahualcóyotl Mayek; Pérez, Elizabeth; Corrêa, Bruno Bordallo; Pérez, Carmen Luz Marrero; Gómez, Consuelo; Sánchez, Verónica Adriana Galván; Pérez, D.Guillermo

doi:10.3109/03009742.2016.1153140

Cited by 4 publications

(8 citation statements)

References 25 publications

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“…Recently, our group also showed that DLE diminishes the inflammatory and degenerative processes of cartilage by increasing the synthesis of proteoglycans, promoting collagen formation and the induction of tissue repair in a murine model of osteoarthritis [8]. DLE reduced the NF- κ B nuclear translocation, thus promoting the expression of the anti-inflammatory cytokines PDGF and FGF-2 [8]. These evidences strongly suggest that DLE regulates the inflammatory process in some diseases [8].…”

Section: Discussionmentioning

confidence: 98%

“…In vitro, DLE regulates the NF- κ B activity and reduces TNF- α and TGF- B 1 secretion, downregulating the HIV transcription [11]. Recently, our group also showed that DLE diminishes the inflammatory and degenerative processes of cartilage by increasing the synthesis of proteoglycans, promoting collagen formation and the induction of tissue repair in a murine model of osteoarthritis [8]. DLE reduced the NF- κ B nuclear translocation, thus promoting the expression of the anti-inflammatory cytokines PDGF and FGF-2 [8].…”

Section: Discussionmentioning

confidence: 99%

“…DLE reduced the NF- κ B nuclear translocation, thus promoting the expression of the anti-inflammatory cytokines PDGF and FGF-2 [8]. These evidences strongly suggest that DLE regulates the inflammatory process in some diseases [8]. …”

Section: Discussionmentioning

confidence: 99%

“…The immunizations were done with 5 mg of MRAG emulsified with 0.5 ml of Freund's complete adjuvant (FCA). After the second immunization (15 days), groups of 5 animals were formed and treated by oral administration with DLE, dialyzable leucocyte extract from Crocodylus moreletii [8], (0.133 mg/Kg/day) (DLE group), Dexamethasone (0.15 mg/kg/day) (Dexamethasone group), or Glycine (50 mg/kg/day) (Placebo group), daily during 34 days. A control group with the pathology without any treatment (WOT group) and a healthy group of rats that did not receive any kind of manipulation (normal group) were included.…”

Section: Methodsmentioning

confidence: 99%

“…For example, Elocalcitol, a vitamin D receptor agonist, decreases the inflammatory process by diminishing the expression of IFN- γ and IL-17 and by inhibiting CD4+ and CD8+ prostate infiltrate in a murine model [7]. Dialyzable Leukocyte Extract (DLE) has been used for the treatment of many inflammatory diseases, like osteoarthritis [8], autoimmune [9], bacterial [10], and viral [11] diseases among others. Pizza et al in 1996 showed that DLE promoted cancer remissions and increased the survival of prostatic cancer patients [12].…”

Section: Introductionmentioning

confidence: 99%

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Anti-Inflammatory Effect of Dialyzable Leukocyte Extract in Autoimmune Prostatitis: Evaluation in Animal Model

Pérez-Alvarado

Gómez

Reyes

et al. 2017

BioMed Research International

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Objective. To evaluate the anti-inflammatory properties of Dialyzable Leukocyte Extract (DLE) in a murine model of chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS). Methods. Histopathological characterization, prostatein Enzyme-Linked Immunosorbent Assay, and immunohistochemical analysis for CD45, TNF-α, IFN-γ, IL-6, IL-17, and IL-4 molecules were done in prostatic Wistar rats treated with DLE, placebo, or Dexamethasone. Results. Histopathological analysis of animals induced to prostatitis showed inflammatory infiltrate, mainly constituted by leucocytes and mast cells as well as Benign Prostatic Hyperplasia. Serum prostatein concentrations were 14 times higher than those displayed by healthy animals. After DLE and Dexamethasone treatments, the inflammatory infiltrate decreased; the tissue morphology was similar to that of a normal prostate, and the prostatein decreased to the basal levels of healthy animals. DLE treatment produced a decreased expression of the cell surface marker CD45 and the proinflammatory cytokines TNF-α, IFN-γ, IL-6, and IL-17. On the other hand, the expression of anti-inflammatory cytokine IL-4 increased in both the Dexamethasone and DLE groups. Conclusion. DLE is able to modulate the inflammatory response in Experimental Autoimmune Prostatitis (EAP).

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Anti-Inflammatory Effect of Dialyzable Leukocyte Extract in Autoimmune Prostatitis: Evaluation in Animal Model

Pérez-Alvarado

Gómez

Reyes

et al. 2017

BioMed Research International

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Anti‐inflammatory effect of omega unsaturated fatty acids and dialysable leucocyte extracts on collagen‐induced arthritis in DBA/1 mice

Pérez-Martínez

Rojas-Espinosa

Hernández-Chávez

et al. 2020

Int J Experimental Path

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SummaryRheumatoid arthritis is a disabling autoimmune disease with a high global prevalence. Treatment with disease‐modifying anti‐arthritic drugs (DIMARDs) has been routinely used with beneficial effects but with adverse long‐term consequences; novel targeted biologics and small‐molecule inhibitors are promising options. In this study, we investigated whether purified omega unsaturated fatty acids (ω‐UFAs) and dialysable leukocyte extracts (DLEs) prevented the development of arthritis in a model of collagen‐induced arthritis (CIA) in mice. We also investigated whether the transcription factor NF‐κB and the NLRP3 inflammasome were involved in the process and whether their activity was modulated by treatment. The development of arthritis was evaluated for 84 days following treatment with nothing, dexamethasone, DLEs, docosahexaenoic acid, arachidonic acid, and oleic acid. Progression of CIA was monitored by evaluating clinical manifestations, inflammatory changes, and histological alterations in the pads’ articular tissues. Both DLEs and ω‐UFAs led to an almost complete inhibition of the inflammatory histopathology of CIA and this was concomitant with the inhibition of NF‐kB and the inhibition of the activation of NLRP3. These data suggest that ω‐UFAs and DLEs might have NF‐κB as a common target and that they might be used as ancillary medicines in the treatment of arthritis.

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Efecto inmunomodulador y anti-inflamatorio de la profilaxis con el Extracto Dializable de Leucocitos (DLEP) en un modelo murino de influenza A

Pérez¹,

Ocampo²,

Cabello³

et al. 2022

JHS

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Anti-inflammatory effect of dialysable leucocyte extract in a rat model of osteoarthritis: histopathological and molecular characterization

Abstract: Our findings suggest that DLE treatment modifies the OA process, promoting the expression of anti-inflammatory cytokines and diminishing the inflammatory effects, avoiding the nuclear translocation of NF-κB in chondrocytes.

Cited by 4 publications

References 25 publications

Anti-Inflammatory Effect of Dialyzable Leukocyte Extract in Autoimmune Prostatitis: Evaluation in Animal Model

Anti-Inflammatory Effect of Dialyzable Leukocyte Extract in Autoimmune Prostatitis: Evaluation in Animal Model

Anti‐inflammatory effect of omega unsaturated fatty acids and dialysable leucocyte extracts on collagen‐induced arthritis in DBA/1 mice

Efecto inmunomodulador y anti-inflamatorio de la profilaxis con el Extracto Dializable de Leucocitos (DLEP) en un modelo murino de influenza A

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