Anti‐Inflammatory Effect of Dental Pulpa Mesenchymal Stem Cell Exosomes Loaded Mucoadhesive Hydrogel on Mice with Dental Nickel Hypersensitivity

Abstract

In this study, the anti‐inflammatory effect of dental pulp MSC exosomes loaded to mucoadhesive hydrogel was investigated in a dental nickel hypersensitivity murine model. After culture of dental pulp MSCs in the third passage MSCs were loaded to a mucoadhesive hydrogel based on chitosan, cross‐linked with genipin and modified with catechol groups. A dental nickel hypersensitivity model was created by administering NiCl2 and 10 μg/mL LPS to 4‐6‐week‐old Balb‐c mice by intradermal injection. In mice treated with dental pulp MSC exosomes and exosomes in hydrogel, IFN‐γ secreting CD4+T lymphocyte ratios significantly increased compared to the untreated group (p<0.05). IFN‐γ and IL‐10 expression in buccal mucosa tissue sections and IFN‐γ secreting CD4+T lymphocyte ratios were found to be significantly higher in mice treated with DPMKH exosomes and DPMKH exosomes in hydrogel compared to the untreated group (p<0.05). According to flow cytometry results, IL‐4 secreting CD4+T lymphocytes were found to be significantly decreased in DPMKH exosomes group compared to dental nickel hypersensitivity group (p<0.05). Administration of DPMKH exosomes with mucoadhesive hydrogel may be an alternative to current medication in the treatment of dental nickel hypersensitivity.This article is protected by copyright. All rights reserved