Anti-Inflammatory Effect of Crude <i>Momordica charantia L.</i> Extract on 2,4,6-Trinitrobenzene Sulfonic Acid-Induced Colitis Model in Rat and the Bioaccessibility of its Carotenoid Content

Ünal, Nalan Gülşen; Kozak, Ayşegül; Karakaya, Sibel; Oruç, Nevin; Barutçuoğlu, Burcu; Aktan, Çağdaş; Sezak, Murat; Özütemiz, Ahmet Ömer

doi:10.1089/jmf.2019.0124

Cited by 11 publications

(9 citation statements)

References 30 publications

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“…MoC extract and dissolved SS were administered to the rats using an oral gavage tube (p.o.) The dose of the MoC extract was selected based on a former study that demonstrated the protective effect of MoC on the Crohn's colitis model (10). However, SS, which is the standard drug for the treatment of ulcerative colitis, was used as a reference to evaluate the effectiveness of MoC (2).…”

Section: Animal Experimental Proceduresmentioning

confidence: 99%

“…Momordica charantia L. (MoC), also known as bitter gourd, bitter apple, or bitter melon, is a green to yellow-coloured flowering plant and a member of the Cucurbitaceae family. MoC widely grows in Asia and the Mediterranean area, and the main phytochemical ingredients of MoC include essentially flavonoids, phenolic acids, cucurbitane-type triterpenoid saponins (momordicine, momordin, and momordicoside (8)(9)(10). Standard constituents of MoC are charantin (a mixture of 5.…”

Section: Introductionmentioning

confidence: 99%

“…Standard constituents of MoC are charantin (a mixture of 5. 25-stigmasteryl glucoside and β-sitosteryl glucoside) and momordicine, which are steroidal saponins (8)(9)(10)(11). Momordicine and charantin are mainly responsible for the beneficial activities and bitterness of MoC (8)(9)(10)(11)(12).…”

Section: Introductionmentioning

confidence: 99%

“…25-stigmasteryl glucoside and β-sitosteryl glucoside) and momordicine, which are steroidal saponins (8)(9)(10)(11). Momordicine and charantin are mainly responsible for the beneficial activities and bitterness of MoC (8)(9)(10)(11)(12). MoC has been reported to have more biological effects such as antioxidant, anti-apoptotic and anti-inflammatory (9,12,13).…”

Section: Introductionmentioning

confidence: 99%

“…MoC has been shown to alleviate dextran sulfate sodium (DSS)-induced colitis in mice by depressing inflammatory cytokines and increasing regulatory T cells (15). Additionally, MoC has been demonstrated to have significant anti-inflammatory effects on trinitrobenzene (TNBS)-induced colitis that mimics Crohn's disease by reducing myeloperoxidase (MPO) activity and cytokine levels in rats (10).…”

Section: Introductionmentioning

confidence: 99%

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Therapeutic Effects of Momordica charantia L. Ethanolic Extract on Acetic Acid-Induced Ulcerative Colitis in Rats

Özbeyli¹,

Şen²,

Aykaç³

et al. 2021

ejb

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Objective: This study aims to investigate the effect of Momordica charantia L. (MoC) ethanolic extract on ulcerative colitis (UC) and was explored in vitro and in vivo. Materials and Methods:The rats were divided into control (C), saline-treated colitis (AA), MoC extract-treated colitis (AA+MoC), and sulfasalazine (SS)-treated colitis (AA+SS) groups. Colitis was induced by acetic acid. MoC extract, SS or saline were given to the related groups for 3 days. Interleukine-1β, malondialdehyde, glutathione levels, myeloperoxidase activity, bax/bcl-2 ratio, caspase-9 and caspase-3 levels were measured in colon and macroscopic and histopathologic examinations were done. Total phenolic/flavonoid content and biological activity of MoC were evaluated by in vitro analysis.Results: Compared to the control group, with acetic acid application interleukin-1β levels, myeloperoxidase activity, malondialdehyde levels, bax/bcl-2 ratio, caspase-9 and caspase-3 levels were significantly upregulated, while glutathione levels were significantly decreased in the AA group. In contrast, MoC and SS treatments reduced interleukin-1β, malondialdehyde levels, myeloperoxidase activity, bax/bcl-2 ratio, and caspase-9 and caspase-3 levels. Glutathione levels increased upon MoC or SS treatment. Increased macroscopic and microscopic scoring with AA improved with MoC or SS treatment, but the MoC or SS treated groups had higher score values than the control. Also, in vitro results showed that MoC exhibited 2,2-diphenyl-1picrylhydrazyl and 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid radical scavenging activity as well as significant antilipoxygenase activity. In addition, MoC extract showed a potent anti-inflammatory activity compared to standard indomethacin. Conclusion:Our biochemical, in vitro and histopathologic analysis indicate that MoC is likely to prove beneficial in UC therapy.

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Section: Animal Experimental Proceduresmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%