Anti-Inflammatory Compounds from Vietnamese Piper bavinum

Abstract

This study reports the anti-inflammatory activity-guided fractionation of the aerial part of Piper bavinum C. CD. (Piperaceae) that led to the isolation of eight secondary metabolites (1–8). The chemical structures of 1–8 were established mainly by NMR and mass spectra. Compound 5 was isolated from P. bavinum for the first time. All the isolated compounds were evaluated against LPS-induced NO production in macrophage RAW 264.7 cells in vitro. Among them, compound 4 showed the most potent inhibitory activity against the LPS-induced NO production with an IC50 value of 5.2 μM followed by compound 5 that inhibited NO production with an IC50 value of 13.5 μM. In the protein levels, compound 4 suppressed LPS-induced COX-2 and iNOS expressions in a dose-dependent manner. The results suggested that P. bavinum and its constituents might exert anti-inflammatory effects.

Anti-inflammatory activities of compounds isolated from Amanita caesarea collected in Lam Dong province

“…From our results, the flavonoid (+)-catechin (2) strongest inhibited NO production (4.8 ± 0.2 μM), followed by (−)-epicatechin (3) exhibited inhibitory effects with an IC50 value of 5.7 ± 0.5 μM, presumably because of the presence of the 3,4-hydroxylation(s) in the benzene ring (Figure 1) and this result is similar to those of previously reported [22][23][24]. Meanwhile, the inhibitory activities on NO production of compounds 4 and 5 were significantly reduced (IC50 values of 18.4 ± 1.2 and 9.6 ± 0.8 μM, respectively) and compound 1 was inactive, presumably because of the lack of 3-hydroxylation (compounds 1 and 4) of the benzene ring or the presence of the methoxy groups (compound 5) in benzene ring (Figure 1) [22][23][24][37][38][39]. These results suggested that flavan-3-ol bearing the 3,4-hydroxylation of the benzene ring could be considered as new lead compounds for the development of agents against NO production.…”

Study on chemical composition and α‐glucosidase inhibitory activity from the leaves of Muntingia calabura

“…The NMR data of 2 showed good similarity with those of trans ‐phytol. [ 14 ] Therefore, 2 was indicated as trans ‐phytol.…”

“…By chromatography methods, including column chromatography and thin layer chromatography, seven compounds were isolated from the methanolic extract of M. calabura leaves. They were 5-(δtocopherol-5'-yl)-δ-tocopherol (1), [13] trans-phytol (2), [14] ergosta-4,6,8 (14),22-tetraen-3-one (3), [15] ergosterol peroxide (4), [16] stigmasta-4-ene-3,6-dione (5), [17,18] 6-hydroxystigmast-4-en-3-one (6), [19] and β-sitosterol (7) (figure 1). [20] This is the first time that compounds 1-6 have been reported for Muntingia genus.…”