Anti-inflammatory, antinociceptive and anti-angiogenic activities of a phospholipid mixture purified from porcine lung tissues

Abstract

This work aimed to assess anti-inflammatory and related properties of a phospholipid mixture purified from porcine lung tissues, named KT&G101, which is being developed as a novel topical remedy for atopic dermatitis. KT&G101 consists of pure phospholipids, mainly phosphatidylcholine (PC) and other phospholipids such as phosphatidylinositol (PI) and phosphatidylserine (PS). Its predominant PC species is 1,2-dipalmitoylphosphatidylcholine (DPPC). KT&G101 exhibited an anti-angiogenic activity in the chick chorioallantoic membrane (CAM) assay. Oral administration of KT&G101 at the dosages of 100, 200 and 400 mg/kg body weight gave rise to an inhibition of 15.4%, 25.3% and 30.1% in the vascular permeability assay, respectively. In the carrageenan-induced inflammation in the air pouches, KT&G101 significantly diminished the volume of exudates in the pouches, the number of polymorphonuclear leukocytes and nitrite content in exudates. In the acetic acid-induced writhing response, oral administration of KT&G101 at the dosages of 50, 100 and 200 mg/kg body weight showed the reduction of 21.6%, 51.6% and 60.8% in the pain response of mice, respectively. It was also able to diminish the nitric oxide (NO) and reactive oxygen species (ROS) levels in the lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells. KT&G101 displayed a significant suppression on the induction of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in the stimulated RAW264.7 cells. However, the free radical scavenging activity of KT&G101 was detected to be very weak in the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. Taken together, KT&G101 possesses anti-inflammatory and related antinociceptive and anti-angiogenic activities, which indirectly supports its use as an anti-atopic therapy.

Glutathione Protects Brain Endothelial Cells from Hydrogen Peroxide-Induced Oxidative Stress by Increasing Nrf2 Expression

“…The 1×10 6 cells/ml were seeded in the plate and were treated with H 2 O 2 or/and GSH for 24 h. Then, b END.3 cells were treated with 5 µM DCF-DA for 30 min at 37℃, and after washing with PBS, the fluorescence was measured in a microscope (Nikon TS100-F ECLIPSE) equipped with a CCD camera (Hamamatsu Photonics, Shizuoka, Japan) [30]. …”

Hair Growth Promoting Potential of Phospholipids Purified from Porcine Lung Tissues

“…The plausible hair growth promoting activity of BP201 was incidentally observed in our previous work employing BALB/c mouse as an animal model of contact dermatitis ( Jung et al ., 2012 ). To revalidate this possibility, the hair growth promoting effect of BP201 was systematically tested using BALB/c mouse.…”

“…In previous studies, BP201 was shown to alleviate symptoms of 2,4-dinitrofluorobenzene-induced allergic contact dermatitis in mice ( Moon et al ., 2012 ). BP201 has anti-inflammatory and related antinociceptive and anti-angiogenic activities, which indirectly support its use as an anti-atopic dermatitis therapy ( Jung et al ., 2012 ). Indeed, the phase III clinical trial for use of BP201 as an anti-atopic dermatitis treatment has been completed, and a BP201-containing product is expected to be commercially available in the near future.…”

Phospholipid fraction has protective effect by improving inflammation and skin barrier function in ischemia-reperfusion injury in mice

“…In addition, Arachidonoyl-lysoPC (Hung et al 2009) and docosahexaenoyl-lysoPC (Huang et al 2010) were found to exert anti-inflammatory effects through inhibition of the plasma leakage in mouse models of zymosan A-induced peritonitis. This indicates that a mixture of phospholipids such as TPF could be one of the requirements for effective anti-inflammatory activity (Jung et al 2012).…”

“…Additionally, TPF suppresses the production of IL-2, IL-4, and IgE, and promotes epidermal tissue regeneration in an atopic dermatitis animal model (Moon et al 2012). TPF also inhibits macrophage recruitment and exhibits anti-inflammatory and anti-nociceptive effects (Jung et al 2012;Moon et al 2012).…”