Anti-inflammatory and nephroprotective activity of Juglans mollis against renal ischemia–reperfusion damage in a Wistar rat model

Abstract

Background Oxidative stress and the inflammatory process are involved in ischemia–reperfusion (I/R) injury. Juglans mollis has been reported as having antioxidant activity, which could attenuate the damage caused by I/R. We evaluated whether a methanolic extract of Juglans mollis (JM) exhibits nephroprotective activity in a Wistar rat model of I/R injury. Methods Four groups of six rats were used: Sham, I/R, JM, and JM + I/R. Two groups were dosed with JM (300 mg/kg) for 7 days before I/R. I/R injury was induced by clamping the renal hilums for 45 min and then reperfusing the kidneys for 15 h. Blood samples were taken to evaluate the levels of alanine aminotransferase (ALT), blood urea nitrogen, creatinine, superoxide dismutase (SOD), malondialdehyde (MDA), interleukin 1β (IL-1β), IL-6, and tumor necrosis factor α (TNF-α). Results The levels of creatinine, ALT, MDA, IL-1β, IL-6, and TNF-α were lower in JM + I/R than in I/R rats, whereas SOD level only was higher in JM + I/R than in Sham rats. No biochemical or histological damage was observed in JM rats compared with Sham rats; however, less histological damage was observed in JM + I/R rats compared with I/R rats. Conclusions To our knowledge, this is the first report of nephroprotective activity of J. mollis against damage induced by I/R. This activity may be related to decreased levels of proinflammatory cytokines (IL-1β, IL-6, and TNF-α) and modulation of oxidative stress markers (SOD and MDA) observed in the present study.

Effect of sodium (S)-2-hydroxyglutarate in male, and succinic acid in female Wistar rats against renal ischemia-reperfusion injury, suggesting a role of the HIF-1 pathway

“…Periods of ischemia of 45 min ( Kang et al, 2005 ) and 60 min ( Nakagawa et al, 2005 ) have been widely reported in the literature. We decided to use a protocol of 45 min of ischemia and 15 h of reperfusion based on the results of previous studies in our laboratory, which have shown that this protocol causes a significative injury, but is mild enough to identify compounds with moderate nephroprotective activity, allowing us to study deeply their potential pharmacologic activity ( Cura-Esquivel et al, 2018 ; Perez-Meseguer et al, 2019 ; Torres-González et al, 2018 ).…”

Long Noncoding RNA/Circular RNA-miRNA-mRNA Axes in Ischemia-Reperfusion Injury

“…Renal IRI, which contributes greatly to AKI, is one of the most critical issues for many clinical situations, including renal transplantation, nephrectomy, sepsis, and repair of suprarenal aneurism [ 129 – 131 ]. Hu et al [ 132 ] used a microarray assay to find that 2218 genes are differentially expressed in renal IRI, including 1103 upregulated genes and 1115 downregulated genes.…”

Comparison of the protective effects of selective endothelin-a receptor antagonist, ambrisentan, and dual endothelin-A/B receptor antagonist, bosentan, in experimental renal ischemia reperfusion injury

“…Similarly, Cong G et al (24) as well as Hong X et al demonstrated an increase in SOD, GSH-Px and CAT activity in treatment groups using a polysaccharide from the roots of Dipsacus asperoides (25). Moreover, Gawel S et al (26) reported that luteoline increases SOD and CAT activity in RIR injury, suggesting that luteolin reduces RIR injury and oxidative stress (27). Taking all into consideration, the fi ndings of our study are consistent with previous studies such that SOD and GSH-Px activities are higher in ambrisentan and bosentan groups compared to the control group.…”

“…Both Aboutaleb N et al (23) and Hong X et al (25) found elevated MDA levels in untreated ischemia groups. Furthermore, in their study investigating the effect of Juglans mollissin on renal ischemia reperfusion injury, Perez-Meseguer J et al (27) reported that only MDA level in the treatment group is lower compared to that in the ischemia reperfusion group. Similar to previous studies, MDA levels in our study were found to be lower in both abrisentan and bosentan groups as compared to the control group although the differences among the groups did not reach the level of signifi cance.…”