Anti-inflammatory and antioxidant effects of hydro-alcoholic extract of Dicliptera cuneata Nees aerial parts

,; Suresh, Parepalli

doi:10.6026/973206300191193

Cited by 1 publication

(1 citation statement)

References 13 publications

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“…The in vitro anti-inflammatory effects of methanol and n-hexane extracts were examined using a protein denaturation assay, based on BSA (bovine serum albumin) (pH 7, ≥98%, Sigma-Aldrich), as reported by Suresh, P [ 68 ]. Briefly, 0.2% BSA in Tris-HCl buffer (pH 6.8, Trizma ® hydrochloride, >99%, Sigma-Aldrich) was combined with various extract concentrations (250, 125, 62.50, 31.20, 15.62, and 7.81, 3 μg/mL) solubilized in distilled water or the reference anti-inflammatory drug (diclofenac, DICLAMID ® , 25 mg/mL).…”

Section: Methodsmentioning

confidence: 99%

Exploring the Therapeutic Potential of Ammodaucus leucotrichus Seed Extracts: A Multi-Faceted Analysis of Phytochemical Composition, Anti-Inflammatory Efficacy, Predictive Anti-Arthritic Properties, and Molecular Docking Insights

Djehiche,

Benzidane,

Djeghim

et al. 2024

Pharmaceuticals

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Ammodaucus leucotrichus exhibits promising pharmacological activity, hinting at anti-inflammatory and anti-arthritic effects. This study investigated seed extracts from Ammodaucus leucotrichus using methanol and n-hexane, focusing on anti-inflammatory and anti-arthritic properties. The methanol extract outperformed the n-hexane extract and diclofenac, a reference anti-inflammatory drug, in trypsin inhibition (85% vs. 30% and 64.67% at 125 μg/mL). For trypsin inhibition, the IC50 values were 82.97 μg/mL (methanol), 202.70 μg/mL (n-hexane), and 97.04 μg/mL (diclofenac). Additionally, the n-hexane extract surpassed the methanol extract and diclofenac in BSA (bovine serum albumin) denaturation inhibition (90.4% vs. 22.0% and 51.4% at 62.5 μg/mL). The BSA denaturation IC50 values were 14.30 μg/mL (n-hexane), 5408 μg/mL (methanol), and 42.30 μg/mL (diclofenac). Gas chromatography–mass spectrometry (GC–MS) revealed 59 and 58 secondary metabolites in the methanol and n-hexane extracts, respectively. The higher therapeutic activity of the methanol extract was attributed to hydroxyacetic acid hydrazide, absent in the n-hexane extract. In silico docking studies identified 28 compounds with negative binding energies, indicating potential trypsin inhibition. The 2-hydroxyacetohydrazide displayed superior inhibitory effects compared to diclofenac. Further mechanistic studies are crucial to validate 2-hydroxyacetohydrazide as a potential drug candidate for rheumatoid arthritis treatment.

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Section: Methodsmentioning

confidence: 99%