Anti-inflammatory and antioxidant effects of a combination of cannabidiol and moringin in LPS-stimulated macrophages

Rajan, Thangavelu Soundara; Giacoppo, Sabrina; Iori, Renato; Nicola, Gina Rosalinda De; Grassi, Gianpaolo; Pollastro, Federica; Bramantı, Placido; Mazzon, Emanuela

doi:10.1016/j.fitote.2016.05.008

Cited by 93 publications

(78 citation statements)

References 29 publications

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“…A pretreatment with antioxidant, anti-inflammatory, or anticancer compounds can improve their survival and their differentiation capacity. Phytocompounds, such as Cannabidiol and Moringin, were widely studied for their antioxidant [18], anti-inflammatory [16,19], and anticancer [23] proprieties and so a treatment with MOR and CBD of mesenchymal cells can improve their survival. Mesenchymal stem cells can spontaneously differentiate to different cell lineage, showing osteogenic, adipogenic, chondrogenic, and neurogenic differentiation capabilities.…”

Section: Discussionmentioning

confidence: 99%

“…Also CBD, the active phytocannabinoid extract from Cannabis sativa , was largely studied for its anti-inflammatory and antioxidant proprieties. Our research in anti-inflammatory activity of CBD was conducted both in macrophage culture [18] and in human gingiva-derived mesenchymal stem cells (hGMSCs) [19]. The effects of these two compounds have always been investigated when they are used alone and not in combination.…”

Section: Introductionmentioning

confidence: 99%

“…Our only research in which the effects of combined treatment had been investigated, had been performed on macrophages rather than on stem cells. In this previous research, we had seen the anti-apoptotic effect of combined use of MOR and CBD on LPS-stimulated macrophages, by immunohistochemical analysis for detection of Bax and Bcl-2 expression, noting a decreased level of Bax, Bcl-2, and caspase-3 [18]. …”

Section: Introductionmentioning

confidence: 99%

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Treatment of Periodontal Ligament Stem Cells with MOR and CBD Promotes Cell Survival and Neuronal Differentiation via the PI3K/Akt/mTOR Pathway

Cariccio

Scionti

Raffa

et al. 2018

IJMS

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Periodontal ligament mesenchymal stem cells (hPDLSCs), as well as all mesenchymal stem cells, show self-renewal, clonogenicity, and multi-tissue differentiation proprieties and can represent a valid support for regenerative medicine. We treated hPDLSCs with a combination of Moringin (MOR) and Cannabidiol (CBD), in order to understand if treatment could improve their survival and their in vitro differentiation capacity. Stem cells survival is fundamental to achieve a successful therapy outcome in the re-implanted tissue of patients. Through NGS transcriptome analysis, we found that combined treatment increased hPDLSCs survival, by inhibition of apoptosis as demonstrated by enhanced expression of anti-apoptotic genes and reduction of pro-apoptotic ones. Moreover, we investigated the possible involvement of PI3K/Akt/mTOR pathway, emphasizing a differential gene expression between treated and untreated cells. Furthermore, hPDLSCs were cultured for 48 h in the presence or absence of CBD and MOR and, after confirming the cellular viability through MTT (3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazoliumbromide) assay, we examined the presence of neuronal markers, through immunofluorescence analysis. We found an increased expression of Nestin and GAP43 (growth associated protein 43) in treated cells. In conclusion, hPDLSCs treated with Moringin and Cannabidiol showed an improved survival capacity and neuronal differentiation potential.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Treatment of Periodontal Ligament Stem Cells with MOR and CBD Promotes Cell Survival and Neuronal Differentiation via the PI3K/Akt/mTOR Pathway

Cariccio

Scionti

Raffa

et al. 2018

IJMS

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“…Furthermore, glucoeurocin-derived ITCs contained in rocket were shown to have indirect antioxidant activity by inducing phase-II enzymes that play an important role in the detoxification of electrophiles and subsequent free radical level reduction [39]. More recently, the anti-inflammatory and antioxidant activity of the glucomoringin-derived ITC moringin has been described in LPS-stimulated macrophages when associated with cannabidiol [40]. In agreement with these previous findings, glucomoringin ITCs could thus participate, in association with other compounds such as tocopherols and flavonoids, in the in vivo antioxidant and anti-inflammatory cardiovascular effects evidenced in MOI SHR.…”

Section: Discussionmentioning

confidence: 99%

Moringa oleiferaSeeds Attenuate Vascular Oxidative and Nitrosative Stresses in Spontaneously Hypertensive Rats

Randriamboavonjy

Rio

Pacaud

et al. 2017

Oxidative Medicine and Cellular Longevity

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Moringa oleifera (MOI) is a tree currently used in traditional medicine in tropical Africa, America, and Asia for therapeutic applications in several disorders including arterial hypertension. We previously described a cardiac protective role of a treatment with MOI seeds in spontaneously hypertensive rats (SHR). Here, we investigated the effects of this treatment on oxidative and nitrosative vascular stresses in SHR, with normotensive Wistar Kyoto rats used as controls. Oxidative and nitrosative stresses detected in SHR aortas using the fluorescent dye dihydroethidine and protein nitrotyrosine staining were reduced in MOI-treated SHR aortas. This was associated with a decrease of free 8-isoprostane circulating level, vascular p22phox and p47phox expressions, and SOD2 upregulation. Moreover, circulating nitrites and C-reactive protein, increased in SHR, were both reduced in SHR receiving MOI. This was associated to decrease iNOS and NF-κB protein expressions after MOI treatment. In functional studies, the endothelium-dependent carbachol-induced relaxation was improved in MOI-treated SHR resistance arteries. Oral administration of MOI seeds demonstrates vascular antioxidant, anti-inflammatory, and endothelial protective effects in SHR. Our data support the use of MOI seeds in diet against cardiovascular disorders associated with oxidative stress and inflammation such as hypertension, scientifically validating the use of these seeds in Malagasy traditional medicine.

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“…Plants producing glucosinolates are also equipped with a thio-linked glucose hydrolysis enzyme called myrosinase (thioglucoside glucohydrolase, EC 3.2.3.1) that liberate bioactive molecules such as isothiocyanates and nitriles and in the case Moringa, the principal enzyme hydrolysis product in the seeds is 4(α-Lrhamnosyloxy)-benzyl isothiocyanate (GMG-ITC) or moringin ( Figure 1). To date, moringin obtained through enzyme catalyzed extraction process from plants such as M. oleifera has been shown to have numerous pharmacological effects including as chemoprotective agent against cancer progression and direct antitumoural effects along with antioxidant and antiinflammatory properties both in vitro and in vivo [19][20][21][22][23][24][25]. Despite all these established pharmacology for moringin (2), the anticancer potential of M. stenopetala seeds has not been established and preliminary study on the methanol extract has shown effect only at high doses (500 µg/ml) in vitro when tested against the HepG2 hepatocytes cell line [26].…”

Section: Introductionmentioning

confidence: 99%

Methodology for Rapid Isolation of Moringin: Potential Anticancer Compound from the Seeds of Moringa stenopetala

Habtemariam¹

2017

Pharm Anal Acta

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The seeds of Moringa stenopetala which are known to be rich sources of glucosinolates, primarily glucomoringin, have been shown to have limited anticancer effects when tested in vitro. In the present study, however, the water extract of the seeds obtained following defatting by hexane displayed potent cytotoxic activity against HepG2 (IC 50 , 6.28 ± 0.55 µg/ml) and the SH-SY5Y human neuroblastoma (IC 50 , 9.81 ± 1.30 µg/ml) cells. The methanol extract displayed a weak cytotoxic activity against the HepG2 human hepatocellular cancer cells when tested upto 500 µg/ml, while extraction with hexane yielded the non-cytotoxic fixed oil which fatty acid composition was primarily oleic acid (75%). By monitoring the constituents of the water extract by HPLC and a one-step combiflash chromatographic system using C-18 silica gel system, the principal active constituent (glucomoringin isothiocyanate or moringin; 4(α-L-rhamnosyloxy)-benzyl isothiocyanate) was isolated. Moringin was 6.3 and 2.4 times more potent than etoposide in the HepG2 and SH-Sy5Y cell lines respectively. The isolation of this potent potential anticancer compound from the seeds of M. stenopetala without the need for multi-step chromatographic and enzyme-digestionbased purification steps are discussed.

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Anti-inflammatory and antioxidant effects of a combination of cannabidiol and moringin in LPS-stimulated macrophages

Cited by 93 publications

References 29 publications

Treatment of Periodontal Ligament Stem Cells with MOR and CBD Promotes Cell Survival and Neuronal Differentiation via the PI3K/Akt/mTOR Pathway

Treatment of Periodontal Ligament Stem Cells with MOR and CBD Promotes Cell Survival and Neuronal Differentiation via the PI3K/Akt/mTOR Pathway

Moringa oleiferaSeeds Attenuate Vascular Oxidative and Nitrosative Stresses in Spontaneously Hypertensive Rats

Methodology for Rapid Isolation of Moringin: Potential Anticancer Compound from the Seeds of Moringa stenopetala

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