Anti-inflammatory and Antioxidant Effects of Japanese Herbal Medicine Kyoseihatekigan on Vocal Fold Wound Healing

Ozawa, Satomi; Mukudai, Shigeyuki; Kaneko, Mitsuaki; Kinoshita, Shingo; Hashimoto, Keiko; Sugiyama, Yoichiro; Hashimoto, Subaru; Akaki, Junji; Hirano, Shigeru

doi:10.1016/j.jvoice.2021.10.003

Cited by 3 publications

(3 citation statements)

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“…AB staining with hyaluronidase digestion was performed to identify HA, which was stained blue. EVG staining was used to identify collagen deposition, which was stained red 21,22 . The BZ‐X700 analyzer software automatically quantifies the area of color in each section.…”

Section: Methodsmentioning

confidence: 99%

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Tamoxifen Alters TGF‐β1/Smad Signaling in Vocal Fold Injury

et al. 2022

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ObjectivesEffective treatments for vocal fold fibrosis remain elusive. Tamoxifen (TAM) is a selective estrogen receptor modulator and was recently reported to have antifibrotic actions. We hypothesized that TAM inhibits vocal fold fibrosis via altered transforming growth factor beta 1 (TGF‐β1) signaling. Both in vitro and in vivo approaches were employed to address this hypothesis.MethodsIn vitro, vocal fold fibroblasts were treated with TAM (10−8 or 10−9 M) ± TGF‐β1 (10 ng/ml) to quantify cell proliferation. The effects of TAM on genes related to fibrosis were quantified via quantitative real‐time polymerase chain reaction. In vivo, rat vocal folds were unilaterally injured, and TAM was administered by oral gavage from pre‐injury day 5 to post‐injury day 7. The rats were randomized into two groups: 0 mg/kg/day (sham) and 50 mg/kg/day (TAM). Histological changes were examined on day 56 to assess tissue architecture.ResultsTAM (10−8 M) did not affect Smad3, Smad7, Acta2, or genes related to extracellular matrix metabolism. TAM (10−8 or 10−9 M) + TGF‐β1, however, significantly increased Smad7 and Has3 expression and decreased Col1a1 and Acta2 expression compared to TGF‐β1 alone. In vivo, TAM significantly increased lamina propria area, hyaluronic acid concentration, and reduced collagen deposition compared to sham treatment.ConclusionsTAM has antifibrotic potential via the regulation of TGF‐β1/Smad signaling in vocal fold injury. These findings provide foundational data to develop innovative therapeutic options for vocal fold fibrosis.Level of EvidenceNA Laryngoscope, 133:2248–2254, 2023

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Section: Methodsmentioning

confidence: 99%

“…EVG staining was used to identify collagen deposition, which was stained red. 21,22 The BZ-X700 analyzer software automatically quantifies the area of color in each section. The percentage of the stained area for each stain was calculated as the density.…”

Section: In Vivo Experimentationmentioning

confidence: 99%

Tamoxifen Alters TGF‐β1/Smad Signaling in Vocal Fold Injury

et al. 2022

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“…Surgery was conducted as described previously on 10 tenweek-old male rats [24][25][26][27][28]. The right side of the rat vocal folds was entirely stripped using a 22-gauge needle, and the lamina propria was removed using micro-forceps until the thyroarytenoid (TA) muscle was visible, which has been confirmed to create severe scar of the vocal fold at 2 months after the stripping.…”

Section: In Vivo Surgical Proceduresmentioning

confidence: 99%

Histological Assessment of The Regenerative Potential of Platelet-Rich Plasma for Severe Vocal Fold Scar in A Rat Model

Mukudai

2023

GJO

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Objectives: Platelet-rich plasma (PRP) is enriched in growth factors and has been expected for tissue regeneration in several organs. Some investigators suggest regenerative potentials of PRP for human vocal fold scar, although there has been lack of animal studies on histology. We aimed to histologically reveal the possible regenerative effects of PRP for severe vocal fold scar model of rats.Methods: Four samples of PRP were obtained from 18-week-old male Sprague-Dawley rats. The concentration of platelet-derived growth factor-BB (PDGF-BB), hepatocyte growth factor (HGF), transforming growth factor-beta (TGF-β), and basic fibroblast growth factor (bFGF) in PRP was determined using ELISA. The vocal folds of 10-week-old male SD rats were unilaterally injured, and PRP or saline (sham) was injected into the scarred vocal folds on post-injury day 56. The degree of vocal fold fibrosis was assessed on day 140 after the injection using histology.Results: ELISA showed variation of growth factors contained in each sample of PRP. The animal study for the treatment by one of the PRP lots indicated significantly increased hyaluronic acid deposition (p = 0.026) and reduced collagen deposition (p = 0.037) in the lamina propria of the vocal fold compared with the sham group. Conclusion:The current study suggested that intracordal injection of PRP may have the regenerative potential for severe vocal fold scar at least on histological aspect. However, it should be kept in mind that the constitution of PRP always differs, which warrants the needs to prepare PRP which contains uniform composition of growth factors.

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