Anti-inflammatory and antioxidant activity of the hot water-soluble polysaccharides from Anacyclus pyrethrum (L.) Lag. roots

Kerboua, Keltoum Alliouche; Benosmane, Lilia; Namoune, Selma; Ouled-Diaf, Khaoula; Ghaliaoui, Nora; Bendjeddou, Dalila

doi:10.1016/j.jep.2021.114491

Cited by 15 publications

(7 citation statements)

References 43 publications

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“…Polysaccharides are macromolecular polymers composed of at least ten monosaccharides linked by glycosidic bonds ( 16 ), and they are natural macromolecular active substances widely found in animals, plants, algae, and microbial cells ( 17 ). Polysaccharides are well-known for their health benefits, such as immunomodulatory ( 18 ), anti-tumor ( 19 ), anti-inflammatory ( 20 ), anti-oxidant ( 21 ), and hypolipidemic effects ( 22 ). Previous studies have suggested that polysaccharides from plants, such as Angelica sinensis ( 23 ), Schisandra ( 24 ), and Ophiopogon ( 25 ), show great lipid-lowering effects.…”

Section: Introductionmentioning

confidence: 99%

Crude Polysaccharide Extracted From Moringa oleifera Leaves Prevents Obesity in Association With Modulating Gut Microbiota in High-Fat Diet-Fed Mice

Wen

et al. 2022

Front. Nutr.

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Moringa oleifera is a commonly used plant with high nutritional and medicinal values. M. oleifera leaves are considered a new food resource in China. However, the biological activities of M. oleifera polysaccharides (MOP) in regulating gut microbiota and alleviating obesity remain obscure. In the present study, we prepared the MOP and evaluated its effects on obesity and gut microbiota in high-fat diet (HFD)-induced C57BL/6J mice. The experimental mice were supplemented with a normal chow diet (NCD group), a high-fat diet (HFD group), and HFD along with MOP at a different dose of 100, 200, and 400 mg/kg/d, respectively. Physiological, histological, biochemical parameters, genes related to lipid metabolism, and gut microbiota composition were compared among five experimental groups. The results showed that MOP supplementation effectively prevented weight gain and lipid accumulation induced by HFD, ameliorated blood lipid levels and insulin resistance, alleviated the secretion of pro-inflammatory cytokines, and regulated the expression of genes related to lipid metabolism and bile acid metabolism. In addition, MOP positively reshaped the gut microbiota composition, significantly increasing the abundance of Bacteroides, norank_f_Ruminococcaceae, and Oscillibacter, while decreasing the relative abundance of Blautia, Alistipes, and Tyzzerella, which are closely associated with obesity. These results demonstrated that MOP supplementation has a protective effect against HFD-induced obesity in mice, which was associated with reshaping the gut microbiota. To the best of our knowledge, this is the first report on the potential of MOP to prevent obesity and modulating gut microbiota, which suggests that MOP can be used as a potential prebiotic.

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Section: Introductionmentioning

confidence: 99%

Crude Polysaccharide Extracted From Moringa oleifera Leaves Prevents Obesity in Association With Modulating Gut Microbiota in High-Fat Diet-Fed Mice

Wen

et al. 2022

Front. Nutr.

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“…The IR spectrogram of PFRAAB was obtained by the potassium bromide (KBr) method according to Alliouche's methods with modification [ 14 ]. Briefly, a total of 100 mg dry KBr was added to the PFRAAB powder (2.0 mg), and then the mixture was pressed and screened by a Nicolet 6700 Fourier Transform Infrared Reflection (FTIR) spectrometer (Thermo Fisher Scientific, USA) at 4000–500 cm −1 at a resolution of 2 cm −1 .…”

Section: Methodsmentioning

confidence: 99%

A Water-Soluble Polysaccharide from the Fibrous Root of Anemarrhena asphodeloides Bge. and Its Immune Enhancement Effect in Vivo and in Vitro

Huang

Wang

Zong

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Background. The fibrous roots of Anemarrhena asphodeloides Bge. (FRAAB) are byproducts of the rhizome of Anemarrhena asphodeloides. Some studies have revealed secondary metabolic small molecules in FRAAB, but there are few reports on the polysaccharides of FRAAB (PFRAAB). Aim of the Study. The present study aimed to investigate the preliminary characterization and underlying mechanism of immune stimulation of PFRAAB. Materials and Methods. The crude polysaccharide of FRAAB was obtained by hot water extraction and alcohol precipitation, and PFRAAB was purified by a diethylaminoethyl-52 (DEAE-52) cellulose chromatographic column and graphene dialysis membrane. The preliminary characterization of PFRAAB was studied by ultraviolet (UV) scanning and Fourier Transform Infrared Reflection (FTIR). The molecular weight and composition of PFRAAB were analysed by high-performance gel permeation chromatography (HPGPC) and high-performance liquid chromatography (HPLC), respectively. The immune stimulation of PFRAAB was investigated by using cyclophosphamide- (CCP-) treated mice and RAW264.7 cells. Results. A water-soluble PFRAAB was obtained with a molecular weight of 115 kDa and was mainly composed of arabinose (ara), galactose (gal), glucose (glc), and mannose (man). Compared with CCP-induced mice, PFRAAB significantly ( p < 0.05 or p < 0.01 ) increased the spleen and thymus index, ameliorated injury to the spleen and thymus, and evaluated immunoglobulin levels. In addition, PFRAAB also increased the secretion of nitric oxide (NO), interleukin-1β (IL-1β), tumour necrosis factor-α (TNF-α), and IL-6 in RAW264.7 cells and upregulated the expression of toll-like receptor 4 (TLR4), Myd88, nuclear factor kappa-B (NF-κB) P65, p–NF–κB P65, IKB-α, and p-IKB-α. Conclusion. PFRAAB possesses immune stimulation activity and can be used as a potential resource for immune-enhancing drugs. Our present study provides a scientific basis for the comprehensive development of Anemarrhena asphodeloides medicinal plant resources.

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“…The Sevag method is commonly used to remove proteins from crude polysaccharide solutions, which is based on the principle of protein denaturation in chloroform and other organic solvents (17). In brief, the crude polysaccharide solution is blended with Sevag's reagent (1-butanol/ chloroform, 1/4 v/v) at a certain proportion, the mixture is shaken well, and the supernatant is collected after centrifugation to remove some of the proteins (37,38). The Sevag method offers advantages of mild conditions, without causing the denaturation of polysaccharides (39), and can achieve a good effect of removing proteins from the crude P. frutescens polysaccharide solution after multiple repeated operations (20,23,34).…”

Section: Perilla Frutescensmentioning

confidence: 99%

Recent trends in extraction, purification, structural characterization, and biological activities evaluation of Perilla frutescens (L.) Britton polysaccharide

Zhu,

Guan,

Wang

et al. 2024

Front. Nutr.

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Perilla frutescens (L.) Britton is an annual herb plant of the Perilla genus in the Labiatae family, which is commonly utilized as an edible and medicinal resource. Polysaccharides are among the major components and essential bioactive compounds of P. frutescens, which exhibit a multitude of biological activities, including antioxidant, antitumor, anti-fatigue, immunoregulation, hepatoprotective, anti-inflammatory, and lipid-lowering effects. As a natural carbohydrate, P. frutescens polysaccharide has the potential to be utilized in the development of drugs and functional materials. In this paper, we provide an overview of progress made on the extraction, purification, structural characterization, and bioactivity of polysaccharides from different parts of P. frutescens. The challenges and opportunities for research are discussed, along with the potential development prospects and future areas of focus in the study of P. frutescens polysaccharides.

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Anti-inflammatory and antioxidant activity of the hot water-soluble polysaccharides from Anacyclus pyrethrum (L.) Lag. roots

Cited by 15 publications

References 43 publications

Crude Polysaccharide Extracted From Moringa oleifera Leaves Prevents Obesity in Association With Modulating Gut Microbiota in High-Fat Diet-Fed Mice

Crude Polysaccharide Extracted From Moringa oleifera Leaves Prevents Obesity in Association With Modulating Gut Microbiota in High-Fat Diet-Fed Mice

A Water-Soluble Polysaccharide from the Fibrous Root of Anemarrhena asphodeloides Bge. and Its Immune Enhancement Effect in Vivo and in Vitro

Recent trends in extraction, purification, structural characterization, and biological activities evaluation of Perilla frutescens (L.) Britton polysaccharide

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