Anti-inflammatory and Antinociceptive Activities of the Hydroalcoholic Extract and the Volatile Fraction of Southeastern Brazilian Brown Propolis

Ribeiro, Victor Pena; Símaro, Guilherme Venâncio; Mejía, Jennyfer Andrea Aldana; Arruda, Caroline; Bastos, Jairo Kenupp

doi:10.1007/s43450-020-00122-5

Cited by 6 publications

(13 citation statements)

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“…Several biological properties were attributed to Brazilian brown propolis, such as leishmanicidal, [8] anti‐inflammatory and nociceptive, [9] mutagenic and antimicrobial, [10] and antimycoplasma [11] . Some studies attribute the antimicrobial activity of brown propolis to terpenic compounds present in its extract, [12] while its antioxidant effect is related to the cytotoxic activity, mediated via free radical‐scavenging activity by the phenolic compounds [13] .…”

Section: Introductionmentioning

confidence: 99%

Phytochemical, Antiplasmodial, Cytotoxic and Antimicrobial Evaluation of a Southeast Brazilian Brown Propolis Produced by Apis mellifera Bees

Ribeiro

Arruda

Mejía

et al. 2021

Chemistry & Biodiversity

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Seven phenolic compounds (ferulic acid, caffeic acid, 4‐methoxycinnamic acid, 3,4‐dimethoxycinnamic acid, 3‐hydroxy‐4‐methoxybenzaldehyde, 3‐methoxy‐4‐hydroxypropiophenone and 1‐O,2‐O‐digalloyl‐6‐O‐trans‐p‐coumaroyl‐β‐D‐glucopyranoside), a flavanonol (7‐O‐methylaromadendrin), two lignans (pinoresinol and matairesinol) and six diterpenic acids/alcohol (19‐acetoxy‐13‐hydroxyabda‐8(17),14‐diene, totarol, 7‐oxodehydroabietic acid, dehydroabietic acid, communic acid and isopimaric acid) were isolated from the hydroalcoholic extract of a Brazilian Brown Propolis and characterized by NMR spectral data analysis. The volatile fraction of brown propolis was characterized by CG‐MS, composed mainly of monoterpenes and sesquiterpenes, being the major α‐pinene (18.4 %) and β‐pinene (10.3 %). This propolis chemical profile indicates that Pinus spp., Eucalyptus spp. and Araucaria angustifolia might be its primary plants source. The brown propolis displayed significant activity against Plasmodium falciparum D6 and W2 strains with IC50 of 5.3 and 9.7 μg/mL, respectively. The volatile fraction was also active with IC50 of 22.5 and 41.8 μg/mL, respectively. Among the compounds, 1‐O,2‐O‐digalloyl‐6‐O‐trans‐p‐coumaroyl‐β‐D‐glucopyranoside showed IC50 of 3.1 and 1.0 μg/mL against D6 and W2 strains, respectively, while communic acid showed an IC50 of 4.0 μg/mL against W2 strain. Cytotoxicity was determined on four tumor cell lines (SK‐MEL, KB, BT‐549, and SK‐OV‐3) and two normal renal cell lines (LLC‐PK1 and VERO). Matairesinol, 7‐O‐methylaromadendrin, and isopimaric acid showed an IC50 range of 1.8–0.78 μg/mL, 7.3–100 μg/mL, and 17–18 μg/mL, respectively, against the tumor cell lines but they were not cytotoxic against normal cell lines. The crude extract of brown propolis displayed antimicrobial activity against C. neoformans, methicillin‐resistant Staphylococcus aureus, and P. aeruginosa at 29.9 μg/mL, 178.9 μg/mL, and 160.7 μg/mL, respectively. The volatile fraction inhibited the growth of C. neoformans at 53.0 μg/mL. The compounds 3‐hydroxy‐4‐methoxybenzaldehyde, 3‐methoxy‐4‐hydroxypropiophenone and 7‐oxodehydroabietic acid were active against C. neoformans, and caffeic and communic acids were active against methicillin‐resistant Staphylococcus aureus.

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Section: Introductionmentioning

confidence: 99%

Phytochemical, Antiplasmodial, Cytotoxic and Antimicrobial Evaluation of a Southeast Brazilian Brown Propolis Produced by Apis mellifera Bees

Ribeiro

Arruda

Mejía

et al. 2021

Chemistry & Biodiversity

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“…Brown propolis from Minas Gerais sample displayed sesquiterpenes (33.62%), oxygenated sesquiterpenes (26.98%), and oxygenated monoterpenes (18.99%) (Ribeiro et al 2021a , b , c ). Quantification differences were observed for samples from this state, although the sesquiterpenes β-caryophyllene and α-copaene ( 13 ) were predominant.…”

Section: Discussionmentioning

confidence: 99%

“…Quantification differences were observed for samples from this state, although the sesquiterpenes β-caryophyllene and α-copaene ( 13 ) were predominant. 1,8-Cineol ( 14 ), terpineol-4-ol ( 15 ), nerolidol ( 16 ), spathulenol ( 17 ), δ-cadinene, aromadendrene ( 18 ), γ-muurolene ( 19 ), and the alkyl-phenylketone, acetophenone have also been reported (Lima et al 2019 ; Olegário et al 2019 ; Ribeiro et al 2021a , b , c ; Símaro, et al 2021).…”

Section: Discussionmentioning

confidence: 99%

“…The botanical origins of some Brazilian propolis were well-established, as Baccharis dracunculifolia for green propolis and Dalbergia ecastaphyllum and Symphonia globulifera for red propolis. Several plants have been described as responsible for their composition regarding brown propolis, as Pinus spp., B. dracunculifolia , Eucalyptus spp., and Araucaria angustifolia (Freitas et al 2011 ; Frota et al 2021 ; Ribeiro et al 2021a , b , c ; Santos et al 2021 ; Serafim et al 2022 ).…”

Section: Discussionmentioning

confidence: 99%

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Brazilian Brown Propolis: an Overview About Its Chemical Composition, Botanical Sources, Quality Control, and Pharmacological Properties

Ribeiro

Mejía²,

Rodrigues³

et al. 2023

Rev. Bras. Farmacogn.

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Brazil is one of the largest propolis producers in the world. Propolis is produced by bees from plant exudates and tissues, leading to many variations in the types of propolis. Generally, Brazilian propolis types are green, brown, and red. Despite not being the main research focus as the green and red propolis, brown propolis is the second most produced propolis type in Brazil and has tremendous economic and medicinal importance. Propolis has drawn attention with the rise in the search for healthier lifestyles, functional foods, biocosmetics, and natural products as therapeutic sources. This review covers the main chemical constituents identified in different types of Brazilian brown propolis, and their botanical sources, chemistry, and biological activities. The economic aspect of brown propolis is also presented. There are many gaps to be filled for brown propolis regarding the development of analytical methods, and quality control to allow its standardization, limiting its applicability in the food and pharmaceutical industries. Future perspectives regarding brown propolis research were discussed, especially biological activities, to support the medicinal uses of different types of brown propolis. Graphical Abstract Supplementary Information The online version contains supplementary material available at 10.1007/s43450-023-00374-x.

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“…Volatile compounds make up 10 % in propolis extracts and are important in the chemical and biological evaluation of propolis [7] . Thus, Ribeiro et al ., [144] evaluated the volatile oil of brown propolis and observed inhibition of flagellar motility in the promastigote form of L. amazonensis in a dose‐dependent manner with an IC 50 value of 21.3 μg/mL. Against amastigote forms, the IC 50 value was 25.1 μg/mL.…”

Section: Protozoamentioning

confidence: 99%

Antiparasitic Properties of Propolis Extracts and Their Compounds

Paula

Cândido

Santos

et al. 2021

Chemistry & Biodiversity

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Propolis is a bee product that has been used in medicine since ancient times. Although its anti‐inflammatory, antioxidant, antimicrobial, antitumor, and immunomodulatory activities have been investigated, its anti‐parasitic properties remain poorly explored, especially regarding helminths. This review surveys the results obtained with propolis around the world against human parasites. Regarding protozoa, studies carried out with the protozoa Trypanosoma spp. and Leishmania spp. have demonstrated promising results in vitro and in vivo. However, there are fewer studies for Plasmodium spp., the etiological agent of malaria and less so for helminths, particularly for Fasciola spp. and Schistosoma spp. Despite the favorable in vitro results with propolis, helminth assays need to be further investigated. However, propolis has shown itself to be an excellent natural product for parasitology, thus opening new paths and approaches in its activity against protozoa and helminths.

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Anti-inflammatory and Antinociceptive Activities of the Hydroalcoholic Extract and the Volatile Fraction of Southeastern Brazilian Brown Propolis

Cited by 6 publications

References 33 publications

Phytochemical, Antiplasmodial, Cytotoxic and Antimicrobial Evaluation of a Southeast Brazilian Brown Propolis Produced by Apis mellifera Bees

Phytochemical, Antiplasmodial, Cytotoxic and Antimicrobial Evaluation of a Southeast Brazilian Brown Propolis Produced by Apis mellifera Bees

Brazilian Brown Propolis: an Overview About Its Chemical Composition, Botanical Sources, Quality Control, and Pharmacological Properties

Antiparasitic Properties of Propolis Extracts and Their Compounds

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