Anti-Inflammatory and Anti-Oxidant Activity of Portulaca oleracea Extract on LPS-Induced Rat Lung Injury

Abstract

Acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) are classified as two lung complications arising from various conditions such as sepsis, trauma, and lung inflammation. Previous studies have shown that the extract of the leaves of Portulaca oleracea (PO) possesses anti-inflammatory and anti-oxidant activities. In the present study, the effects of PO (50–200 mg/kg) and dexamethasone (Dexa; 1.5 mg/kg) on lipopolysaccharide (LPS)-induced ALI were investigated. Subsequentially, the lung wet/dry ratio; white blood cells (WBC); levels of nitric oxide (NO); myeloperoxidase (MPO); malondialdehyde (MDA); thiol groups formation; super oxide dismutase (SOD) and catalase (CAT) activities; and levels of interleukin (IL)-1β, tumor necrosis factor (TNF)-α, IL-6, IL-10, prostaglandin E2 (PGE2), and transforming growth factor (TGF)-β in the broncho alveolar lavage fluid (BALF) were evaluated in order to demonstrate the anti-oxidant and anti-inflammatory activity of PO. Our results show that PO suppresses lung inflammation by the reduction of IL-β, IL-6, TNF-α, PGE2, and TGF-β, as well as by the increase of IL-10 levels. We also found that PO improves the level of WBC, MPO, and MDA, as well as thiol group formation and SOD and CAT activities, compared with the LPS group. The results of our investigation also show that PO significantly decreased the lung wet/dry ratio as an index of interstitial edema. Taken together, our findings reveal that PO extract dose-dependently displays anti-oxidant and anti-inflammatory activity against LPS-induced rat ALI, paving the way for rational use of PO as a protective agent against lung-related inflammatory disease.

Antiinflammatory and anti‐cancer activities of pomegranate and its constituent, ellagic acid: Evidence from cellular, animal, and clinical studies

“…ALI and acute respiratory distress syndrome (ARDS) are two major inflammatory lung complications with a high rate of morbidity and mortality (Baradaran Rahimi et al, ; Boskabadi, Askari, Hosseini, & Boskabady, ). Severe pulmonary inflammation, massive presence of neutrophils and lymphocytes in interstitial tissue, edema, disruption of epithelial integrity, and the injury of lung parenchyma are the most common features of these diseases (Baradaran Rahimi et al, ; Boskabadi et al, ).…”

Cytotoxicity and apoptogenic properties of the standardized extract of Portulaca oleracea on glioblastoma multiforme cancer cell line (U-87): a mechanistic study

“…The plant was identified by Mr. Jouharchi, and a voucher of a sample was served as references in the herbarium of the school of pharmacy at Mashhad University of Medical Sciences (Deposition/Herbarium No: 12-1615-240). The leaves were dried in shadow and powdered and extraction was performed by the maceration method (Askari et al 2016[ 7 ]; Boskabady et al, 2016[ 18 ], Baradaran Rahimi et al, 2019[ 16 ]). 100 g of leaves powder was soaked with 1 lit 70 % ethanol for 48 h at controlled room temperature.…”

“…The Iranian traditional medicine name of this plant is Qorfeh and also named Purslane in the English language. In ancient medical texts such as Qanoon e tib by Shaik Bu-Ali-Seena and Makhzan Ul Adviya by Mohammad Hussein Aghili Alavi Khorasani, it has been stated that PO could be used for fever, cramps, fumigation and burnt as well as inflammatory skin rash (Okwuasaba et al, 1987[ 42 ]; Rasheed et al, 2004[ 46 ]; Mohanapriya et al, 2006[ 38 ]; Baradaran Rahimi et al, 2019[ 16 ]). Moreover, PO is imported as a medicinal herb in the WHO due to its most benefits (Lim and Quah, 2007[ 36 ]).…”

“…Moreover, PO is imported as a medicinal herb in the WHO due to its most benefits (Lim and Quah, 2007[ 36 ]). In previous studies, many pharmacological properties of PO have been demonstrated including anti-cancer, anti-inflammatory and strengthening the immune system (Yang et al, 2008[ 59 ], Zhu and Wu, 2009[ 66 ], Baradaran Rahimi et al, 2019[ 16 ]). Also, PO blocked the HepG2 and HeLa cells proliferation (Chen et al, 2010[ 21 ]).…”

Protective effects of hesperetin on lipopolysaccharide-induced acute lung injury in a rat model

“…[4] Lipopolysaccharide (LPS) located in the Gram-negative bacterial wall structure is released extracellularly and is considered the predominant microbial inducer of inflammation, as it initiates the innate immune response at the onset of ALI. [5,6] Therefore, in preclinical experimental studies, LPS is widely used as an effective strategy in the formation of the clinically relevant form of ALI in experimental animal models. [1,7] The inflammatory cell infiltration and inflammation, which play a major role in the pathogenesis of ALI and are highly responsible for the occurrence of tissue damage, are also associated with overexpression of proinflammatory cytokines.…”