Anti-inflammatory and anti-osteoarthritis effects of fermented Achyranthes japonica Nakai

Lee, Sung‐Gyu; Lee, Eun‐Ju; Park, Woo-Dong; Kim, Jong-Boo; Kim, Eun‐Ok; Choi, Sang-Won

doi:10.1016/j.jep.2012.05.020

Cited by 54 publications

(35 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…25 LPS could activate inflammatory response in vitro and in vivo by enhancing the expressions of proinflammatory cytokines, F I G U R E 5 MALAT1 alleviated LPS-induced ATDC5 cell inflammatory injury by upregulating miR-19b. 27,28 In this study, LPS treatment dramatically inhibited murine chondrogenic ATDC5 cell viability, induced ATDC5 cell apoptosis, and enhanced proinflammatory cytokines expression. B-D, After 5 μg/mL LPS treatment and/or pEX-MALAT1 (miR-19b inhibitor) transfection, the viability of ATDC5 cells, apoptosis of ATDC5 cells, and expression levels of Bcl-2, Bax, pro-caspase 3, cleaved-caspase 3, pro-caspase 9, and cleaved-caspase 9 in ATDC5 cells were measured using CCK-8 assay, annexin V-PE staining, and Western blot analysis, respectively.…”

Section: 4 | Discussionmentioning

confidence: 53%

Retracted: Long noncoding RNA MALAT1 alleviates lipopolysaccharide‐induced inflammatory injury by upregulating microRNA‐19b in murine chondrogenic ATDC5 cells

Lin

Liu

Zhao

et al. 2018

J of Cellular Biochemistry

View full text Add to dashboard Cite

Osteoarthritis is the most frequent chronic bone-joint disease in middle-aged and older people worldwide. This study investigated the effects of long noncoding RNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) on lipopolysaccharide (LPS)-induced murine chondrogenic ATDC5 cell inflammatory injury. Cell viability and apoptosis were assessed using cell counting kit-8 assay and annexin V-phycoerythrin (PE) staining, respectively. The expression levels of interleukin-1β (IL)-1β, IL-6, IL-8, tumor necrosis factor α (TNF-α), MALAT1, and microRNA-19b (miR)-19b were measured using quantitative reverse-transcription polymerase chain reaction. Enzyme-linked immunosorbent assay was conducted to detect the concentrations of IL-1β, IL-6, IL-8, and TNF-α in culture supernatant of ATDC5 cells. Expressions of key factors involved in cell apoptosis, proinflammatory response, Wnt/β-catenin, and nuclear factor κB (NF-κB) pathways were analyzed using Western blot analysis. We found that LPS treatment remarkably induced ATDC5 cell apoptosis and inflammatory injury. MALAT1 was upregulated in LPS-stimulated ATDC5 cells. Overexpression of MALAT1 significantly reversed the LPS-induced ATDC5 cell inflammatory injury, while suppression of MALAT1 had opposite effects. Further results showed that MALAT1 positively regulated the expression of miR-19b in ATDC5 cells. Knockdown of miR-19b reversed the protective effect of MALAT1 on LPS-induced ATDC5 cells. In addition, MALAT1 reduced LPS-induced Wnt/β-catenin and NF-κB pathways activation in ATDC5 cells by upregulating miR-19. To conclude, our research verified that MALAT1 alleviated LPS-induced ATDC5 cell inflammatory injury by upregulating miR-19b and inactivating Wnt/β-catenin and NF-κB pathways. This finding will be helpful for further understanding the critical roles of MALAT1 and miR-19b in osteoarthritis and may provide possible targets for osteoarthritis diagnosis and treatment.

show abstract

Section: 4 | Discussionmentioning

confidence: 53%

Retracted: Long noncoding RNA MALAT1 alleviates lipopolysaccharide‐induced inflammatory injury by upregulating microRNA‐19b in murine chondrogenic ATDC5 cells

Lin

Liu

Zhao

et al. 2018

J of Cellular Biochemistry

View full text Add to dashboard Cite

show abstract

“…The anti-inflammatory effects of fermented AJ have been mentioned previously [16]. However, the earlier reports did not elucidate the molecular mechanisms, such as MAPK signaling, which directly regulate the inflammation process.…”

Section: Discussionmentioning

confidence: 91%

“…The powder (100 g) was extracted three times with 75% ethanol. FAJ was fermented using a previously described method [16]. Briefly, AJ was mixed with distilled water (DW) and autoclaved at 120°C for 20 min.…”

Section: Extraction and Fermentation Of Ajmentioning

confidence: 99%

“…Achyranthes japonica Nakai (AJ) has been popularly used as traditional medicine in East Asia for the treatment of chronic diseases [15]. AJ contains bioactive compounds, including inokosterone, ecdysterone, oleanolic acid, bisdesmoside and saponins, which are known to possess scavenging effects against free radicals [16,17]. Reports suggest that AJ extract has potent activities against inflammation and osteoarthritis owing to its bioactive compounds [15].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of the Antioxidant and Anti-Inflammatory Potential of Fermented Achyranthes japonica Nakai Extract

Mijan¹,

Park²,

Lee³

et al. 2018

Nat Prod Chem Res

View full text Add to dashboard Cite

Achyranthes japonica Nakai (AJ) is a medicinal plant and has well-known health benefits. This study has evaluated the antioxidant and anti-inflammatory activities of the fermented extract of AJ (FAJ). Fermentation of the extract increased the total phenolic and flavonoid contents of AJ. FAJ showed significantly higher free radical scavenging activities as compared with NFAJ. In addition, FAJ exhibited higher ferric (Fe 3+ ) reducing activity than NFAJ. FAJ inhibited the production of nitric oxide (NO) and prostaglandin E2 (PGE2) in lipopolysaccharide (LPS)induced RAW 264.7 cells at a higher level than NFAJ without affecting the cell viability. The mRNA expression of tumor necrosis factor α (TNF-α) in RAW 264.7 cells was substantially reduced by FAJ treatment. Moreover, the upregulation of pp38 and protein kinase RNA-like endoplasmic reticulum kinase (pERK) by LPS in RAW 264.7 cells was markedly suppressed by FAJ. These results suggest that FAJ has therapeutic potential as an antioxidant and anti-inflammatory agent.

show abstract

“…However, available studies on Achyranthes japonica (Miq.) Nakai's anti-inflammatory and anti-arthritic effects remain scarce (13).…”

Section: Introductionmentioning

confidence: 99%

Effect of fermented Achyranthes japonica (Miq.) Nakai extract on osteoarthritis

Kang¹,

Lee²,

Yu³

et al. 2017

Korean Journal of Food Science and Technology

View full text Add to dashboard Cite

The present study was conducted to evaluate the anti-inflammatory and anti-arthritic effects of fermented Achyranthes japonica (Miq.) Nakai extract (FAJE). The FAJE was effective in nitrogen oxide (NO) scavenging in RAW264.7 cells. In the case of experimental Sprague Dawley (SD) rats injected with monosodium iodoacetate (MIA), the levels of TNF-α and IL-1β in blood increased in the osteoarthritis-induced group while decreasing in the group administered with FAJE. In addition, MMP-2 and MMP-9 in cartilage tissues increased in the osteoarthritis-induced group, but decreased in the group treated with FAJE. Cartilage examination indicated that the osteoarthritis-induced group exhibited cartilage erosion and cell degeneration, but in the FAJE administered group the tissue, conditions were recovered and cartilage proteoglycan was increased. Therefore, FAJE clearly showed anti-inflammatory effects and this suggests it is effective for recovery from osteoarthritis induced by MIA.

show abstract

Anti-inflammatory and anti-osteoarthritis effects of fermented Achyranthes japonica Nakai

Cited by 54 publications

References 31 publications

Retracted: Long noncoding RNA MALAT1 alleviates lipopolysaccharide‐induced inflammatory injury by upregulating microRNA‐19b in murine chondrogenic ATDC5 cells

Retracted: Long noncoding RNA MALAT1 alleviates lipopolysaccharide‐induced inflammatory injury by upregulating microRNA‐19b in murine chondrogenic ATDC5 cells

Evaluation of the Antioxidant and Anti-Inflammatory Potential of Fermented Achyranthes japonica Nakai Extract

Effect of fermented Achyranthes japonica (Miq.) Nakai extract on osteoarthritis

Contact Info

Product

Resources

About