Anti-Inflammatory and Anti-Colon Cancer Activities of Mung Bean Grown in Burkina Faso

Abstract

Widely used in traditional medicine in Asia and recently introduced in Burkina Faso under the name Beng-tigré, mung bean is a legume consumed throughout the world and more so in India. The objective of this study was to evaluate the cytotoxicity of the mung bean grown and consumed in Burkina Faso and to study its biological properties such as anti-inflammatory and anticancer activity of the natural and sprouted seeds. The cytotoxicity of the extracts was tested on Artemia salina larvae, and the anti-inflammatory activity was evaluated in vitro by albumin denaturation method using diclofenac as reference molecule. The anticancer activity of hydro-ethanol extracts was evaluated on rats made cancerous with 1,2-dimethylhydrazine (DMH) using 5-fluorouracil as reference molecule. The results showed that the highest yield of the plant extraction was observed with the hydro-ethanol solvent, both for the natural form of mung bean (MBN) and for its sprouted form (MBG). The cytotoxicity test showed no toxicity of the extracts toward shrimp larvae. The ethanolic extract of germinated mung bean seeds gave the highest anti-inflammatory activity at 95.13 ± 0.22% inhibition with significant difference ( p < 0.05 ) between the extracts. Cancer induction with DMH was inhibited by both MBN and MBG extracts. The test of preventive effects of the extracts showed the best activity with significant difference in biochemical results. These results confirm that the mung bean grown in Burkina Faso, as a nontoxic legume, is a functional food that can be integrated into the population’s dietary habits for a double interest. Moreover, they open perspectives for the research of active principles of plant origin with anti-inflammatory and anticancer properties.

Phenolic Profile and Antioxidant, Anti-Inflammatory Activity of <i>Annona senegalensis, Ipomoea batatas, Terminalia superba</i> and <i>Psidium guajava</i> Linn Extracts Used in Benin

“…The cytotoxic effect of the extracts was evaluated following the method described by [23]. The test is a primary toxicity test carried out twice on 48 hours larvae of Artemia salina.…”

Polyphenol Analysis via LC-MS-ESI and Potent Antioxidant, Anti-Inflammatory, and Antimicrobial Activities of Jatropha multifida L. Extracts Used in Benin Pharmacopoeia

“…The anti-inflammatory properties of J. multifida extracts in vitro were assessed using the methodology outlined by Kabré et al [ 28 ]. In essence, a solution was created by combining 100 µL of J. multifida leaf extracts at varying concentrations (ranging from 3.9 to 1000 µg/mL) with 10 µL of egg albumin and 140 µL of phosphate-buffered saline (PBS, pH 6.4).…”

Pharmacological Property and Cytotoxic Effect Showing Antiproliferative Potency in Human Melanoma Cell Lines (A375) of Combretum racemosum P. Beauv. Leaf and Root Extracts Used in Benin

“…The in vitro anti-inflammatory activity of C. racemosum leaf and root extracts was assessed using a method previously described by Kabré et al [36], which involves the use of egg albumin. In this assay, conducted in a 96-well microplate, 100 µL of the extracts (with concentrations ranging from 7.81 to 2000 µg/mL) were combined with 10 µL of egg albumin and 140 µL of phosphate-buffered saline (PBS, pH 6.4).…”