Anti-inflammatory and anti-apoptotic effects of Shaoyao decoction on X-ray radiation-induced enteritis of C57BL/6 mice

Li, Zhuohong; Gao, Ying; Du, Lijun; Yuan, Ye; Huang, Wenbo; Xi, Fu; Huang, Yong‐Liang; Zhang, Xufan; You, Feng-Ming; Li, Shijie

doi:10.1016/j.jep.2022.115158

Cited by 10 publications

(9 citation statements)

References 42 publications

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“…The reproductive function of hens is closely related to the levels of inflammatory factors and apoptosis-related genes in the body, which is particularly important for layer production. Li et al 's study found that Shaoyao decoction improves tissue fibrosis and treats radiation enteritis by reducing inflammation and apoptotic responses (50). The findings of this study suggest that CHMs tend to decrease the activity of caspase-8 enzyme in hens' serum.…”

Section: Discussionmentioning

confidence: 53%

Effects of dietary Chinese herbal mixtures on productive performance, egg quality, immune status, caecal and offspring meconial microbiota of Wenchang breeder hens

Liu,

Huang,

et al. 2023

Front. Vet. Sci.

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This study aimed to evaluate the effects of Chinese herbal mixtures (CHMs) on productive performance, egg quality, immune status, anti-apoptosis ability, caecal microbiota, and offspring meconial microbiota in hens. A total of 168 thirty-week-old Wenchang breeder hens were randomly divided into two groups, with each group comprising six replicate pens of fourteen hens. The groups were fed a basal diet (CON group) and a basal diet with 1,000 mg/kg CHMs (CHMs group) for 10 weeks. Our results showed that dietary supplementation with CHMs increased the laying rate, average egg weight, hatch of fertile, and offspring chicks’ weight while concurrently reducing the feed conversion ratio (FCR) and embryo mortality (p < 0.05). The addition of CHMs resulted in significant improvements in various egg quality parameters, including eggshell strength, albumen height, haugh unit, and the content of docosatetraenoic acid (C20:4n-6) in egg yolk (p < 0.05). The supplementation of CHMs had a greater concentration of IgA and IgG while decreasing the content of IL-6 in serum compared with the CON group (p < 0.05). Addition of CHMs to the diet increased the expression of Bcl-2 and IL-4 in liver and ovary, decreased the expression of IL-1β, Bax, and Caspase-8 in jejunum and ovary, and decreased the expression of NF-κB in liver, jejunum, and ovary (p < 0.05). Moreover, dietary CHMs reduced the abundance of Desulfovibrio in caecal microbiota as well as decreased the abundance of Staphylococcaceae_Staphylococcus and Pseudomonadaceae_Pseudomonas in the offspring meconial microbiota (p < 0.05). In conclusion, the CHMs could improve productive parameters by enhancing immune status, anti-apoptosis capacity, and modulating the caecal microbiota of Wenchang breeder hens, as well as maintaining the intestinal health of the offspring chicks.

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Section: Discussionmentioning

confidence: 53%

Effects of dietary Chinese herbal mixtures on productive performance, egg quality, immune status, caecal and offspring meconial microbiota of Wenchang breeder hens

Liu,

Huang,

et al. 2023

Front. Vet. Sci.

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“…[ 18 ] Studies have shown that radiation can increase intestinal permeability and tight junction damage. [ 14 ] In this study, radiation led to decreased expression of intestinal barrier proteins and increased intestinal permeability. However, NAR treatment significantly mitigated these effects.…”

Section: Discussionmentioning

confidence: 78%

“…It has been shown that an increase in the levels of strong proinflammatory factors, induces inflammatory stimulation caused by ROS generation and continuously erodes and disrupts the intestinal barrier, thus triggering excessive apoptosis and causing upregulation of proapoptosis related genes Bax, Cox4, and Cleaved‐Caspase3 and downregulation of the antiapoptotic gene Bcl‐2, which are involved in mediating multiple pathways of radiation‐induced intestinal injury in the intestinal epithelium. [ 14 ] Our study showed that TAI stimulated the increase of intestinal inflammatory factor IL‐6, IL‐1β, TNFα level in mice, decreased the expression of intestinal barrier protein (Occludin, Claudin1, Claudin3 and ZO‐1), and promoted the expression of Bax and Caspase3. Bax and Cleaved‐Caspase3 are signaling molecules involved in apoptosis responses, aggravating inflammatory damage.…”

Section: Discussionmentioning

confidence: 83%

Naringenin Alleviates Radiation‐Induced Intestinal Injury by Inhibiting TRPV6 in Mice

Ling,

Wang,

Chen

et al. 2024

Molecular Nutrition Food Res

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ScopeNaringenin (NAR) possesses unique anti‐inflammatory, antiapoptosis effects and various bioactivities; however, its role against radiation‐induced intestinal injury (RIII) remains unclear. This study aims to investigate whether NAR has protective effects against radiation‐induced intestinal injury and the underlying mechanisms.Methods and resultsC57BL/6J mice are exposed to a single dose of 13 Gy X‐ray total abdominal irradiation (TAI), then gavaged with NAR for 7 days. NAR treatment prolongs the survival rate, protects crypts and villi from damage, alleviates the level of radiation‐induced inflammation, and mitigates intestinal barrier damage in the irradiated mice. Additionally, NAR reduces immune cell infiltration and intestinal epithelial cell apoptosis. NAR also shows radioprotective effects in human colon cancer cells (HCT116) and human intestinal epithelial cells (NCM460). It reduces cell damage by reducing intracellular calcium ion levels and reactive oxygen species (ROS) levels. NAR‐mediated radioprotection is associated with the downregulation of transient receptor potential vanilloid 6 (TRPV6), and inhibition of apoptosis pathway. Notably, treatment with NAR fails to further increase the protective effects of the TRPV6 inhibitor 2‐APB, indicating that TRPV6 inhibition is essential for NAR activity.ConclusionNAR inhibits the apoptosis pathway by downregulating TRPV6 and reducing calcium ion level, thereby alleviating RIII. Therefore, NAR is a promising therapeutic drug for RIII.

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“…[22] Serum LPS and inflammatory cytokines, such as IL-6 and TNF-𝛼, were elevated in mice treated with 12 Gy TAI. [23] Lactobacillus rhamnosus GG reduced radiation-induced epithelial injury and improved crypt survival in a TLR-2/cyclo-oxygenase-2-dependent manner. [24] In this study, the ameliorated intestinal morphology and serum LPS and DAO concentrations indicate that L. casei ATCC334 protected the integrity and function of the intestinal mucosa, which was also related to reduced TNF-𝛼 and IL-6 secretion.…”

Section: Discussionmentioning

confidence: 99%

Lacticaseibacillus casei ATCC334 Ameliorates Radiation‐Induced Intestinal Injury in Rats by Targeting Microbes and Metabolites

Hua

Zhang

Xu³

et al. 2022

Molecular Nutrition Food Res

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Scope Gastrointestinal side effects are frequently observed in patients receiving medical radiation therapy. As Lacticaseibacillus casei ATCC334 potentially affects microbial ecosystem, the study hypothesizes that it may improve radiation‐induced intestinal injury in rats by modulating the “gut microbiota‐metabolite‐barrier axis.” Methods and results Rats are fed one of three or no doses of L. casei ATCC334 for 7 days and then expose to a single dose of 9 Gy X‐ray total abdominal irradiation. Supplementation with L. casei ATCC334 promote the proliferation of intestinal stem cells (ISCs), increase the expression of tight junction proteins, reduce intestinal permeability, and protect intestinal barrier integrity. Moreover, 16S rRNA sequencing show that medium and high doses of L. casei ATCC334 inhibit the growth of Escherichia/Shigella and favor Akkermansia proliferation. L. casei ATCC334 intervention reprogram the metabolic profile and inhibit putrescine production but promote alpha‐linolenic acid (ALA) production. Notably, a decrease in putrescine and an increase in ALA are significantly correlated with the proliferation of ISCs and enhanced intestinal barrier function following L. casei ATCC334 intervention. Conclusion These results highlight that medium and high doses of L. casei ATCC334 alleviate radiation‐induced intestinal damage by enhancing the mucosal barrier and remodeling the gut microbiota structure and metabolic activity.

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Anti-inflammatory and anti-apoptotic effects of Shaoyao decoction on X-ray radiation-induced enteritis of C57BL/6 mice

Cited by 10 publications

References 42 publications

Effects of dietary Chinese herbal mixtures on productive performance, egg quality, immune status, caecal and offspring meconial microbiota of Wenchang breeder hens

Effects of dietary Chinese herbal mixtures on productive performance, egg quality, immune status, caecal and offspring meconial microbiota of Wenchang breeder hens

Naringenin Alleviates Radiation‐Induced Intestinal Injury by Inhibiting TRPV6 in Mice

Lacticaseibacillus casei ATCC334 Ameliorates Radiation‐Induced Intestinal Injury in Rats by Targeting Microbes and Metabolites

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