Anti-Inflammatory and Analgesic Effects of the Marine-Derived Compound Comaparvin Isolated from the Crinoid Comanthus bennetti

Abstract

To date, no study has been conducted to explore the bioactivity of the crinoid Comanthus bennetti. Here we report the anti-inflammatory properties of comaparvin (5,8-dihydroxy-10-methoxy-2-propylbenzo[h]chromen-4-one) based on in vivo experiments. Our preliminary screening for anti-inflammatory activity revealed that the crude extract of Comanthus bennetti significantly inhibited the expression of pro-inflammatory proteins in lipopolysaccharide (LPS)-stimulated murine RAW 264.7 macrophage cells. Comaparvin isolated from crinoids significantly decreased the expression of inducible nitric oxide synthase (iNOS) protein and mRNA in LPS-stimulated macrophage cells. Moreover, our results showed that post-treatment with comaparvin significantly inhibited mechanical allodynia, thermal hyperalgesia and weight-bearing deficits in rats with carrageenan-induced inflammation. Comaparvin also attenuated leukocyte infiltration and iNOS protein expression in carrageenan-induced inflamed paws. These results suggest that comaparvin is a potential anti-inflammatory therapeutic agent against inflammatory pain.

Anti-inflammatory and burn injury wound healing properties of the shell of Haliotis diversicolor

“…Using this screening model, we have successfully illustrate the several marine-derived substances can produce efficacious anti-inflammatory activity in in vitro and in vivo models [16–19]. Thus, screening for inhibitors of the pro-inflammatory mediators in marine natural bioactive substances is a promising avenue for drug development.…”

AICAR activates AMPK to regulate STAT3 nuclear translocation and phosphorylation and iNOS expression in inflammatory pain

“…The iNOS is increased in in ammatory and neuropathic pain, which promotes the synthesis of nitric oxide to induce the sensitization of peripheral or central sensory nerves and pain [35,36]. Inhibition of iNOS expression in macrophages is involved in alleviating in ammatory pain [37]. In carrageenan induced hindpaw in ammatory pain mice model, angelica dahurica can alleviate in ammatory pain by inhibiting the production of iNOS and nitric oxide in macrophages [38].…”

Marine Pharmacology in 2014–2015: Marine Compounds with Antibacterial, Antidiabetic, Antifungal, Anti-Inflammatory, Antiprotozoal, Antituberculosis, Antiviral, and Anthelmintic Activities; Affecting the Immune and Nervous Systems, and Other Miscellaneous Mechanisms of Action

“…[185][186][187]; two new briarane diterpenoids briaviolides E and I (170,171), isolated from the Taiwanese soft coral Briareum violacea [188]; a new pigmented phenazine compound dermacozine H (172), isolated from the actinomycete Dermacoccus abyssi sp. nov. strain MT1.1, isolated from a Mariana Trench sediment at a depth of 10,898 m [189]; a novel sesquiterpene dysifragilone A (173), isolated from the South China Sea sponge Dysidea fragilis [190]; a new xenicane 4α-hydroxypachylactone (174), isolated from a Chinese brown alga Dictyota plectens [191]; a polyketide comaparvin (175), isolated from the Taiwanese marine crinoid Comanthus bennetti [192]; two novel eunicellin-type diterpenoids hirsutalins N and S (176, 177) and a new tocopherol-derived metabolite hirsutocospiro A (178), isolated from the Taiwanese soft coral Cladiella hirsuta [193][194][195]; a new cembranoid isosinulaflexiolide K (179), isolated from cultured Taiwanese soft corals Sinularia sandensis and Sinularia flexibilis [196]; a novel 9,11-secogorgosteroid klyflaccisteroid F (180), isolated from the Taiwaneses soft coral Klyxum flaccidum [197]; new eunicellin-type diterpenoids krempfielins N (181), Q (182), and R (183), isolated from a Taiwanese soft coral Cladiella krempfi [198,199]; a methylfarnesylquinone (184), isolated from the Taiwanese marine brown alga Homeostrichus formosana [200]; a novel steroid monanchosterol B (185), isolated from the Korean sponge Monanchora sp. [201]; a oxygenated steroid derivative (186), isolated from the Vietnamese starfish Protoreaster nodosus [202]; a new spirobisnaphthalene rhytidenone C (187), isolated from an extract of a cultured fungal endophyte Rhytidhysteron sp.…”

“…Kwan and colleagues reported that the peptide grassypeptolide A (192), isolated from the marine cyanobacterium Lyngbya confervoides, inhibited IL-2 production and proliferation of activated T cells by inhibiting the protease dipeptidyl peptidase 8, probably by binding at inner cavity of the enzyme at two distinct sites [208]. Wang and colleagues isolated a pair of novel bisheterocyclic quinolone-imidazole alkaloids (+)-and ( _ ) spiroreticulatine (193) from the South China sea sponge Fascaplysinopsis reticulata, which showed inhibition of IL-2 production by Jurkat T cells [209].…”