Anti-Inflammatory Activity of <i>Chrozophora rottleri</i> Extracts on Carrageenan-Induced Rat Paw Edema

Abstract

Abstract. The present study was intended to evaluate Anti-inflammatory activity of C. rottleri extracts (Hydroalcoholic, Methanol, Ethyl acetate and Hexane). The Anti-inflammatory activity of C. rottleri extracts at doses of 125mg/kg, 250mg/kg and 500mg/kg using carrageenan induced rat paw edema model compared with standard drug (Indomethacin). The selected plant extracts significantly inhibited paw edema along with the standard drug Indomethacin. Of all extracts, methanol extract produced significant effect on reduction of increased paw thickness, hydro alcoholic and ethyl acetate extracts produced moderate percentage inhibition and hexane extract produced low level of percentage inhibition in reducing paw edema on carrageenan induced rats. In all extracts, methanol extract at a dose of 500mg/kg showed more percentage inhibition i.e . 53.47±2.19. From the results obtained during the study it is concluded that C. rottleri having the bioactive molecule responsible for Anti-inflammatory activity by individually or by combination of different bio-active compounds present in it. Further is necessary for isolation and characterization of bioactive molecules which are responsible for the selected plant biological activities.

Evaluation of Aster bakerianus Burtt Davy ex C.A. Sm. Crude Root Extract for Acute Antiinflammatory Activity in Rats

“…Based on such observations the initiation and progression of oedema after subcutaneous injection of carrageenan into the rat paw has been described as being triphasic and acute (Ishola et al . The early phase is mainly mediated by histamine and serotonin starts immediately after injection of carrageenan while the intermediate phase provides continuity between the early phase and the late phase and is mediated by kinins, mainly bradykinin (Ishola et al, 2014;Popoola et al, 2016).The late phase is associated with release of prostaglandins, leukotrienes and NO with increased expression of COX-2 and iNOS which are influenced by the nuclear factor kappa B (NF-κB) (Di Rosa et al, 1971;Sidhapuriwala et al, 2007) and also the release of protease, lysosomes, and migration of leukocytes into the inflamed site occurs during the late phase of oedema (Talluri et al, 2016). It has been reported that the suppression of carrageenan induced hind paw oedema after the fourth hour correlates reasonably with therapeutic doses of most clinically effective anti-inflammatory drugs, both steroidal and non steroidal such as indomethacin (Panthong et al, 2007;Kumar & Jain, 2014).…”

“…However, these drugs, proven to be effective in many cases, can cause undesirable side effects to some degree (Lucas, 2016) Also, traditional medicines derived from plant extracts are increasingly being used to treat a wide variety of diseases; many being prescribed broadly for the treatment of inflammatory conditions though relatively little knowledge about their mode of action is available (Amala Hazel et al, 2018; Oguntibeju, 2018). While plant derived antiinflammatories are rarely as immediately effective as the steroidal antiinflammatory drugs and NSAIDs, they are very rarely as toxic nore potentially life threatening (Talluri et al, 2016;Oguntibeju, 2018).…”

“…Many plant species have shown potential for antiinflammatory activities (Oguntibeju, 2018) due to the presence of a wide variety of phytochemicals that can be a source of anti-inflammatories themselves and could also be used for the discovery of novel antiinflammatory agents with fewer side effects (Talluri et al, 2016;Oguntibeju, 2018). The chemical compounds present in plant products are a part of the physiological functions of living organisms, and hence they are believed to have better compatibility with the human body (Prasad et al, 2012).…”