Anti-Inflammatory Activity of Cnidoscolus aconitifolius (Mill.) Ethyl Acetate Extract on Croton Oil-Induced Mouse Ear Edema

Abstract

Nowadays, there is a growing interest in the development of medicinal plant-based therapies to diminish the ravages of the inflammatory process related to diseases and tissue damage. Most therapeutic effects of these traditional medicinal plants are owed to their phenolic and antioxidant properties. C. aconitifolius is a traditional medicinal plant in Mexico. Previous characterization reports have stated its high nutritional and antioxidant components. The present study aimed to better understand the biological activity of C. aconitifolius in inflammation response. We developed an ethyl acetate extract of this plant to evaluate its anti-inflammatory capacity and its flavonoid content. The topical anti-inflammatory effect of the ethyl acetate extract of C. aconitifolius was determined by the croton oil-induced mouse ear edema test, while flavonoid detection and concentration were determined by thin layer chromatography and the aluminum chloride colorimetric assay, respectively. Topical application of the extract showed significant inhibition of the induced-ear edema (23.52 and 49.41% for 25 and 50 mg/kg dose, respectively). The extract also exhibited the presence of flavonoids. The finding of the anti-inflammatory activity exerted by the C. aconitifolius and the identification of its active principles may suggest and support its use for inflammation treatment.

The Effects of the Aqueous, Ethanol and Hexane Extracts of Cnidoscolus aconitifolius Leaf on the Hematological Indices and Liver Histological Status of Streptozotocin-induced Diabetic Rats

“…However, there was periportal infiltration of inflammatory cells with mild fibrosis in extract treated groups, however, hepatocytes were moderately congested and hepatic features were preserved and kept (Slide). Padilla-Camberos et al, (2021) expounded the anti-inflammatory properties of Cnidoscolus aconitifolius of which its bioactive components have been implicated.…”

“…Its treatment value ranges from the capacity to improve vision, neutralize scorpion stings, manage insomnia, and eliminate gout (Jensen, 1997, Atuahene et al, 1999. Other studies have demonstrated the application of C. aconitifolius in alcoholism, diabetes mellitus, obesity, kidney stones, hemorrhoids (Diaz-Bolio, 1975;Kuti andTorres, 1996, Mordi, 2012) and antiinflammation (Padilla-Camberos et al, 2021). Recently, the phenolic and amino acid composition of Ethiopian Chaya (Cnidoscolus chayamansa) was documented (Temesgen et al, 2022).…”

Bioactive Compounds from Natural Products: Separation, Characterization, and Applications

“…In [3], the anti-inflammatory activity of Cnidoscolus aconitifolius was presented using an ethyl acetate extract, and the role of flavonoid content was determined in this study. The anti-inflammatory effect of C. aconitifolius extract was determined using the mouse ear edema test induced by the activities of croton oil.…”

Antimicrobial activity of n-hexane and ethyl acetate extracts from Candida tropicalis and Phyllosticta capitalensis fungal endophytes

“…The finding observed from this study may be corroborated by well-established evidence, which suggests the two plants (O. suave and C. aconitifolius) where the endophytes (C. tropicalis and P. capitalensis) were isolated are validated medicinal plants. 26,27 The medicinal plant, O. suave, has been demonstrated to have antioxidant properties due to the high con-tent of flavonoids and polyphenols secondary metabolites, which are said to have cytoprotection effect. 28 Also, essential oil from O. suave was demonstrated to have antimicrobial activity against pathogens.…”