Anti-inflammatory Activity of 1-docosanoyl Cafferate Isolated from<i>Rhus verniciflua</i>in LPS-stimulated BV2 Microglial Cells

Lee, Jae Won; Cheong, Il Young; Kim, Hae-Sung; Lee, Jae Jun; Lee, Yong-Suk; Kwon, Yong-Soo; Kim, Myong Jo; Lee, Hee Jae; Kim, Sung‐Soo; Wang, Chun

doi:10.4196/kjpp.2011.15.1.9

Cited by 17 publications

(22 citation statements)

References 37 publications

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“…Although anti-HIV and anti-cancer activities of betulinic acid and its derivatives have been reported to be through the inhibition of HIV entry, HIV-proteases or reverse transcriptase (Fujioka et al, 1994;Yogeeswari and Sriram, 2005), and through increased degradation of the transcription factors specifi city protein 1 (Sp1), Sp3, and Sp4 (Chintharlapalli et al, 2007), respectively, the underlying mechanism of anti-infl ammatory property of betulinic acid has not been clearly demonstrated (Recio et al, 1995). The present results evidently demonstrate that betulinic acid signifi cantly suppresses the activity of transcription factor NF-kB, which has been reported to play a key role in the infl ammatory responses, through the inhibition of LPS-induced IKB degradation in the cytoplasm (Lee et al, 2011). In accordance with present study, it has been recently reported that betulinic acid exhibits a protective effect in ischemia-induced brain injury by suppressing iNOS expression and oxidative stress (Lu et al, 2011).…”

Section: Discussionsupporting

confidence: 80%

Betulinic Acid Inhibits LPS-Induced MMP-9 Expression by Suppressing NF-kB Activation in BV2 Microglial Cells

Kim

2011

Biomolecules and Therapeutics

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Section: Discussionsupporting

confidence: 80%

Betulinic Acid Inhibits LPS-Induced MMP-9 Expression by Suppressing NF-kB Activation in BV2 Microglial Cells

Kim

2011

Biomolecules and Therapeutics

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“…Hydroxycinnamic acids and their ester derivatives have been reported to possess a variety of pharmacological activities including antitumor, antiviral, anti-inflammatory, immunosuppressive, and neuroprotective activities (Nagasaka et al , 2007; Kim, 2010; Lee et al , 2011b). Although it has been reported that THC exerts inhibitory activity against human neutrophil elastase, which is involved in the regulation of inflammatory processes (Steinbrecher et al , 2008), the biological activity of THC has not been extensively determined.…”

Section: Discussionmentioning

confidence: 99%

“…It has been reported that hydroxycinnamic acids possess various biological properties such as anti-inflammatory, anti-tumor, and neuroprotective activities (Nagasaka et al , 2007; Steinbrecher et al , 2008; Kim, 2010). Ester derivatives of hydroxycinnamic acids have been also reported to exhibit a variety of biological activities (Lee et al , 2011b). Caffeic acid phenethyl ester (CAPE) has been reported to activate HO-1 gene via regulation of Nrf2 (Lee et al , 2010).…”

Section: Introductionmentioning

confidence: 99%

3,4,5-Trihydroxycinnamic Acid Inhibits Lipopolysaccharide-Induced Inflammatory Response through the Activation of Nrf2 Pathway in BV2 Microglial Cells

Lee¹,

Choi²,

Park³

et al. 2013

Biomolecules and Therapeutics

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3,4,5-Trihydroxycinnamic acid (THC) is a derivative of hydroxycinnamic acids, which have been reported to possess a variety of biological properties such as anti-inflammatory, anti-tumor, and neuroprotective activities. However, biological activity of THC has not been extensively examined. Recently, we reported that THC possesses anti-inflammatory activity in LPS-stimulated BV2 microglial cells. However, its precise mechanism by which THC exerts anti-inflammatory action has not been clearly identified. Therefore, the present study was carried out to understand the anti-inflammatory mechanism of THC in BV2 microglial cells. THC effectively suppressed the LPS-induced induction of pro-inflammatory mediators such as NO, TNF-α, and IL-1β. THC also suppressed expression of MCP-1, which plays a key role in the migration of activated microglia. To understand the underlying mechanism by which THC exerts these anti-inflammatory properties, involvement of Nrf2, which is a cytoprotective transcription factor, was examined. THC resulted in increased phosphorylation of Nrf2 with consequent expression of HO-1 in a concentration-dependent manner. THC-induced phosphorylation of Nrf2 was blocked with SB203580, a p38 MAPK inhibitor, indicating that p38 MAPK is the responsible kinase for the phosphorylation of Nrf2. Taken together, the present study for the first time demonstrates that THC exerts anti-inflammatory properties through the activation of Nrf2 in BV2 microglial cells, suggesting that THC might be a valuable therapeutic adjuvant for the treatment of inflammation-related disorders in the CNS.

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“…Cells were pretreated with EOFAZ or Sim for 2 h before coincubation with ox-LDL for 24 h. The production of NO was estimated using a commercially available kit (Beyotime) by measuring the amount of nitrite, a major stable metabolite of NO, using the Griess reagent system, as described elsewhere [46].…”

Section: No Content Measurementmentioning

confidence: 99%

The Endothelial Protective Properties of Essential Oil from Fructus Alpiniae zerumbet via the Akt/NOS-NO Signaling Pathway In Vitro

Xiao

Zeng

et al. 2014

Planta Med

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Oxidized low-density lipoprotein has been demonstrated to induce vascular injury, at least partly, through the nitric oxide-dependent pathway. Our previous studies showed that the essential oil from Fructus Alpiniae zerumbet had a capacity of antioxidation to ameliorate endothelial function and vasodilatation on aortic rings ex vivo. The present study aims to confirm the hypothesis that Fructus Alpiniae zerumbet protects against oxidized low-density lipoprotein-induced endothelial dysfunction in human aortic endothelial cells, and the role nitric oxide synthase plays in the process. Human aortic endothelial cells were pretreated with one of three different doses of Fructus Alpiniae zerumbet (1, 2, or 4 µg/mL), simvastatin (10 µM), or vehicle,followed by coincubation with oxidized low-density lipoprotein (150 µg/mL), for designed time periods. Exposure to oxidized low-density lipoprotein resulted in cytotoxicity accompanied by mitochondrial membrane potential collapse, excessive reactive oxygen species generation, and a decrease of nitric oxide production. The upregulation of inducible nitric oxide synthase mRNA and protein expression was determined by reverse transcription-polymerase chain reaction and Western blot analysis, respectively. Furthermore, oxidized low-density lipoprotein inhibited Akt phosphorylation and downregulated endothelial nitric oxide synthase and soluble guanylatecyclase in mRNA expression and the protein level. However, cytotoxicity and aberrant reactive oxygen species and nitric oxide generation caused by oxidized low-density lipoprotein could be significantly inhibited by Fructus Alpiniae zerumbet or Sim pretreatment. Results from this study confirmed that Fructus Alpiniae zerumbet could protect against oxidized low-density lipoprotein-induced endothelial dysfunction by antioxidation and modulating the Akt/nitric oxide synthase-nitric oxide signaling pathway.

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Anti-inflammatory Activity of 1-docosanoyl Cafferate Isolated fromRhus vernicifluain LPS-stimulated BV2 Microglial Cells

Cited by 17 publications

References 37 publications

Betulinic Acid Inhibits LPS-Induced MMP-9 Expression by Suppressing NF-kB Activation in BV2 Microglial Cells

Betulinic Acid Inhibits LPS-Induced MMP-9 Expression by Suppressing NF-kB Activation in BV2 Microglial Cells

3,4,5-Trihydroxycinnamic Acid Inhibits Lipopolysaccharide-Induced Inflammatory Response through the Activation of Nrf2 Pathway in BV2 Microglial Cells

The Endothelial Protective Properties of Essential Oil from Fructus Alpiniae zerumbet via the Akt/NOS-NO Signaling Pathway In Vitro

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