Anti-inflammatory activities of puerarin in high-fat diet-fed rats with streptozotocin-induced gestational diabetes mellitus

Abstract

To investigate the effect of puerarin on insulin resistance and inflammation in rats with gestational diabetes mellitus (GDM). Gestational diabetic model rats were established by intraperitoneal injection of streptozotocin (25 mg/kg) combined with high-fat feeding and were randomly assigned to three groups: the control group, the GDM group, and the puerarin-treated group. Puerarin was intragastrically administered to rats daily until the offspring were born. The rats in both the GDM group and control group were administered the same volume of normal saline. Serum total cholesterol, triglycerides, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol in all groups of rats were measured. Haematoxylin and eosin staining was used to evaluate morphological changes in the liver, pancreas, and adipose tissues around the reproductive organs. Western blotting was carried out to measure the protein expression of IRS-1 and inflammatory factors, including TNF-α, TLR4, MyD88 and phosphorylated NF-κB, in the adipose tissues around the reproductive organs. Puerarin had preventive effects on GDM-induced pathological changes and ameliorated glucose and lipid metabolism disorders in GDM rats. Puerarin upregulated IRS-1 expression and decreased the protein expression of TNF-α, TLR4, and MyD88 as well as the levels of phosphorylated NF-κB in adipose tissues around the reproductive organs in GDM rats. This study indicated that puerarin exerts anti-inflammatory effects by downregulating the important TLR4/MyD88/NF-κB inflammatory signalling pathway. Therefore, puerarin can decrease the expression of TNF-α and ameliorate insulin resistance in GDM rats, suggesting the potential efficacy of puerarin in GDM treatment.

Protective effect of sinomenine against inflammation and oxidative stress in gestational diabetes mellitus in female rats via TLR4/MyD88/NF‐κB signaling pathway

“…to the development of GDM and oxidative stress. Previous study showed that the omega-3 fatty acid had lipid-lowering potential during the various pathological conditions (Xu et al, 2020;Yang et al, 2017). Our experimental study showed that the GDM group rats exhibited the abnormal alteration in lipid metabolism in plasma.…”

“…GDM disease also boosted the risk of adverse pregnancy outcomes such as spontaneous abortion, fetal dysfunction, stillbirth, macrosomia and intrauterine growth restriction. Congenital abnormalities of fetal, critical organs account for 6%-10% of all pregnancies, a 2-5-fold increase over simple healthy pregnancies (Tian et al, 2015;Xu et al, 2020). Because the causes STZ nitrosourea compound isolated from soil microbes (Streptomyces achromogenes), is a widely used chemical (low and high doses) for inducing diabetes (type I and II) (Kumar, Ahmed, Gupta, Anwar, et al, 2013;Kumar et al, 2020).…”

“…The level of glycosylated hemoglobin can be easily determined in the circulation and has a strong correlation with the level of glycemia (Gothandam et al, 2019;Shah et al, 2019). GDM rats showed an increased level of HbA1c, suggesting widespread damage to proteins and sugars in the circulation, oxidation of sugars, lens proteins, vascular wall, and a continuing increase in the production of free radicals and oxidative stress (Liu et al, 2021;Wang et al, 2019;Xu et al, 2020). SM treatment significantly suppressed HbA1c level, suggesting that SM has a glycation process of protein.…”

Studies of the Anti-Diabetic Mechanism of Pueraria lobata Based on Metabolomics and Network Pharmacology

“…Surprisingly, puerarin, the main active and characteristic marker components for Pueraria lobata in Chinese Pharmacopoeia (The State Pharmacopoeia Commission of China, 2020), exhibited low OB and DL. Nevertheless, puerarin had lipid-and glucoselowering effects in the treatment of obesity and DM [26][27][28], together with anti-inflammation effects, and the improvement of cardiovascular problems [29][30][31]. In summary, it was reasonable to believe that the 19 differentiated compounds could be listed as potential effective pharmacological activities for Pueraria lobata (Table 1).…”

Cytoprotective Effect of Liposomal Puerarin on High Glucose-Induced Injury in Rat Mesangial Cells

“…It is well-known that puerarin protects cells against oxidative stress [ 15 ]. Puerarin can decrease the expression of TNF-α and ameliorate insulin resistance in gestational diabetes mellitus rats by reducing glucose and lipid metabolism disorders [ 16 ]. However, as with other isoflavonoids, puerarin insolubility often limits its bioavailability.…”