Anti-inflammatory activities of Chinese herbal medicine sinomenine and Liang Miao San on tumor necrosis factor-α-activated human fibroblast-like synoviocytes in rheumatoid arthritis

Chen, Dapeng; Wong, Chun‐Kwok; Leung, Ping‐Chung; Fung, Kwok‐Pui; Lau, Clara Bik‐San; Lau, Clara Bik San; Li, Edmund Kwok-Ming; Tam, Lai‐Shan; Lam, Christopher Wai‐Kei

doi:10.1016/j.jep.2011.05.048

Cited by 74 publications

(25 citation statements)

References 56 publications

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“…Innovative pharmaceuticals need further improvement of outcome in osteosarcoma, especially in metastatic management. Sinomenine, traditional Chinese herbal medicine, is known because of its anti-inflammatory effect on arthritis (22)(23)(24). Substantial research has reported that sinomenine has antitumor activity in various malignant tumors.…”

Section: Discussionmentioning

confidence: 99%

Sinomenine prevents metastasis of human osteosarcoma cells via S phase arrest and suppression of tumor-related neovascularization and osteolysis through the CXCR4-STAT3 pathway

Xie

Ren

Lin

et al. 2016

International Journal of Oncology

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Abstract. Osteosarcoma is the most common primary malignant tumor of the bone. The long-term survivals continue to be unsatisfactory for patients with metastatic and recurrent disease. Metastasis is still a severe challenge in osteosarcoma treatment. Sinomenine, an alkaloid from traditional Chinese medicine, has been proved to possess potent antitumor and anti-invasion effect on various cancers. However, the effect of sinomenine on human osteosarcoma and the underlying mechanisms remains unknown. We report here that sinomenine inhibited proliferation by inducing S phase arrest and suppressing the clone formation. Significant inhibitory effects were found in invasion and metastasis in osteosarcoma, but little cytotoxicity was observed in tested concentrations. Exposure to sinomenine resulted in suppression of invasion and migration in osteosarcoma cells as well as tube formation ability in the human umbilical vein endothelial cells (HUVEC) and U 2 OS cells. Furthermore, it demonstrated that CXCR4 played a key role contributing to invasion in osteosarcoma which is considered to be a core target site in sinomenine treatment. Sinomenine inhibited invasion by suppressing CXCR4 and STAT3 phosphorylation then downregulating the expression of MMP-2, MMP-9, RANKL, VEGF downstream. In addition, then RANKL-mediated bone destruction stimulated by osteoclastogenesis and VEGF-related neovascularization were restrained. Importantly, in vivo, sinomenine suppressed proliferation, osteoclastogenesis and bone destruction. Through these various comprehensive means, sinomenine inhibits metastasis in osteosarcoma. Taken together, our results revealed that sinomenine caused S phase arrest, inhibited invasion and metastasis via suppressing the CXCR4-STAT3 pathway and then osteoclastogenesis-mediated bone destruction and neovascularization in osteosarcoma. Sinomenine is therefore a promising adjuvant agent for metastasis control in osteosarcoma.

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Section: Discussionmentioning

confidence: 99%

Sinomenine prevents metastasis of human osteosarcoma cells via S phase arrest and suppression of tumor-related neovascularization and osteolysis through the CXCR4-STAT3 pathway

Xie

Ren

Lin

et al. 2016

International Journal of Oncology

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“…Moreover, that SIN could influence the expression of TNF (Huang et al, 2007;Chen et al, 2011;Yi et al, 2011) and other factors which may inhibit the growth and induce apoptosis has been proved. Just because of this, it was reported that SIN has an antitumor effect and low toxicity (He et al, 2005;Li et al, 2008).…”

Section: Ji-xiang Zhang Et Almentioning

confidence: 99%

“…It has previously been demonstrated that SIN exhibited a wide range of pharmacological actions including anti-inflammatory, anti-rheumatic, immunosuppressive, analgesic and antiarrhythmic effects (Shu et al, 2007;Teng et al, 2012;Song et al, 2013). And there are many other researches indicate that SIN has many pharmacological characters such as inducing apoptosis and anti-angiogenesis effect what has been treated as means of treating colon carcinoma (Kok et al, 2005;Ju et al, 2010;Chen et al, 2011).…”

Section: Suppressive Effect Of Sinomenine Combined With 5-fluorouracimentioning

confidence: 99%

Suppressive Effect of Sinomenine Combined with 5-Fluorouracil on Colon Carcinoma Cell Growth

Zhang¹,

Yang²,

Wu³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Asian Pac J Cancer Prev, 15 (16), 6737-6743 IntroductionColorectal cancer is the fourth most common cancer in the world, and significant international variations in the distribution of colorectal cancer have been observed (Center et al., 2009;Siegal et al., 2013). Meanwhile, colon cancer is more frequent than rectal cancer (Labianca et al., 2010;Di Lena et al., 2013). Recently, the main therapy on primary colon carcinoma is surgical resection, but only half of them can be cured with the rest hopeless. In addition, before the colon carcinoma was diagnosed successfully, the metastasis has already happened. For better prognosis after surgical resection or other clinical situations, chemotherapy as a relatively effective therapy is used more and more.As the first-line chemotherapeutic agent to treat colon carcinoma, 5-fluorouracil (5-FU) has past 40 years (Beretta et al., 2004). Whereas the uncontrollable toxicity and dissatisfying effective rate which around 20-35% with median overall survival no more than 1 year could not meet the needs of treatment (Wu et al., 2013;Focan et al., 2013;Ghiringhelli et al., 2013). So to seek a way such as united therapy that can improve the effective rate and decrease

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“…Many previous studies have demonstrated that sinomenine has anti-inflammatory and immunosuppressive effects [19]–[21]. Cheng et al [22] demonstrated that sinomenine attenuates TNBS-induced colitis and that the therapeutic mechanism might be related to the reduction of up-regulated colonic TNF-α and IFN-γ.…”

Section: Introductionmentioning

confidence: 99%

Effects of Sinomenine on the Expression of microRNA-155 in 2,4,6-Trinitrobenzenesulfonic Acid-Induced Colitis in Mice

Zhu

Zhou

et al. 2013

PLoS ONE

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BackgroundSinomenine, a pure alkaloid isolated in Chinese medicine from the root of Sinomenium acutum, has been demonstrated to have anti-inflammatory and immunosuppressive effects. MicroRNAs (miRNAs) are gradually being recognized as critical mediators of disease pathogenesis via coordinated regulation of molecular effector pathways.Methodology/FindingsAfter colitis was induced in mice by instillation of 5% (w/v) 2,4,6-trinitrobenzenesulfonic acid (TNBS), sinomenine at a dose of 100 or 200 mg/kg was orally administered once daily for 7 days. We evaluated body weight, survival rate, diarrhea score, histological score and myeloperoxidase (MPO) activity. The mRNA and protein expression levels of miR-155, c-Maf, TNF-α and IFN-γ were determined by quantitative RT-PCR and immunohistochemistry, respectively. Sinomenine (100 or 200 mg/kg)-treated mice with TNBS-induced colitis were significantly improved in terms of body weight, survival rate, diarrhea score, histological score and MPO activity compared with untreated mice. Both dosages of sinomenine significantly decreased the mRNA and protein expression levels of c-Maf, TNF-α and IFN-γ, which elevated in TNBS-induced colitis. Furthermore, sinomenine at a dose of 200 mg/kg significantly decreased the level of miR-155 expression by 71% (p = 0.025) compared with untreated TNBS-induced colitis in mice.Conclusions/SignificanceOur study evaluated the effects and potential mechanisms of sinomenine in the anti-inflammatory response via miRNA-155 in mice with TNBS-induced colitis. Our findings suggest that sinomenine has anti-inflammatory effects on TNBS-induced colitis by down-regulating the levels of miR-155 and several related inflammatory cytokines.

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Anti-inflammatory activities of Chinese herbal medicine sinomenine and Liang Miao San on tumor necrosis factor-α-activated human fibroblast-like synoviocytes in rheumatoid arthritis

Cited by 74 publications

References 56 publications

Sinomenine prevents metastasis of human osteosarcoma cells via S phase arrest and suppression of tumor-related neovascularization and osteolysis through the CXCR4-STAT3 pathway

Sinomenine prevents metastasis of human osteosarcoma cells via S phase arrest and suppression of tumor-related neovascularization and osteolysis through the CXCR4-STAT3 pathway

Suppressive Effect of Sinomenine Combined with 5-Fluorouracil on Colon Carcinoma Cell Growth

Effects of Sinomenine on the Expression of microRNA-155 in 2,4,6-Trinitrobenzenesulfonic Acid-Induced Colitis in Mice

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