Anti-inflammatory activities of amber extract in lipopolysaccharide-induced RAW 264.7 macrophages

Tian, Yuan; Zhou, Siqi; Takeda, Reiko; Okazaki, Kazuma; Sekita, Marie; Sakamoto, Kazuichi

doi:10.1016/j.biopha.2021.111854

Cited by 26 publications

(19 citation statements)

References 25 publications

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“…In previous studies, many effects of amber—such as anti-allergic effects [ 17 ], promotion of collagen production, and suppression of melanin production [ 19 ]—have been investigated. In our previous studies, we found that amber extract also has anti-inflammatory effect [ 18 ], the ability to reduce fat accumulation [ 20 ], and can protect cells from amyloid-β1-42-induced cytotoxicity [ 21 ].…”

Section: Discussionmentioning

confidence: 99%

“…Neuroinflammation is also an important part of neurodegenerative diseases such as PD and AD [ 8 , 49 , 50 ]. In our study group, amber had an anti-inflammatory effect on the LPS-induced inflammatory cell model [ 18 ]. In previous studies, kujigamberol, which is isolated from Kuji amber, had anti-allergic effects [ 17 , 29 ].…”

Section: Discussionmentioning

confidence: 99%

“…In Russia, amber is commonly used in folk medicine [ 16 ]. In recent years, the effects of amber on allergy [ 17 ], anti-inflammation [ 18 ], suppression of melanin production, promotion of collagen production [ 19 ], and reduction in fat accumulation [ 20 ] have been identified. A previous study by our group found that amber extract has a protective effect against amyloid-β-induced neuronal cell death [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

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Protective Effect of Amber Extract on Human Dopaminergic Cells against 6-Hydroxydopamine-Induced Neurotoxicity

et al. 2022

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Parkinson’s disease (PD) is the second most common progressive neurodegenerative disease, after Alzheimer’s disease. In our previous study, we found that amber—a fossilized plant resin—can protect cells from apoptosis by decreasing the generation of reactive oxygen species (ROS). In this study, we focused on the effect of amber on 6-hydroxydopamine-induced cell apoptosis in the human neuroblastoma cell line SHSY5Y (one model for PD). Initially, we determined the protective effect of amber on the PD model. We found that amber extract has a protective effect against 6-hydroxydopamine-induced cell apoptosis. The decrease in ROS, cleaved caspase-3, pERK, and extracellular signal-regulated kinase (ERK) protein levels confirmed that amber extract decreases apoptosis via the ROS-mediated ERK signaling pathway. Furthermore, we determined the effects of amber extract on autophagy. The results showed that amber extract increased the levels of LC3II and Beclin-1, suggesting that amber extract can protect neuronal cells against 6-hydroxydopamine-induced cell apoptosis by promoting autophagy.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Protective Effect of Amber Extract on Human Dopaminergic Cells against 6-Hydroxydopamine-Induced Neurotoxicity

et al. 2022

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“…Recently, our research group reported several other benefits of AE in the quest to investigate folkloric beliefs pertaining to its usage. First, Tian et al, 2021, discovered that AE possessed anti-inflammatory properties [19] whilst Luo et al, 2021, reported that AE may be potent in managing Alzheimer's disease [20]. Additionally, Sogo et al, 2021, discovered that AE could reduce fat accumulation [12].…”

Section: Introductionmentioning

confidence: 99%

Stress Buffering and Longevity Effects of Amber Extract on Caenorhabditis elegans (C. elegans)

Somuah-Asante

Sakamoto

2022

Molecules

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Amber is a fossilized tree resin historically used in wound healing and stress relief. Unfortunately, there is no concrete scientific evidence supporting such efficacy. Here, the stress buffering and longevity effect of Amber extract (AE) in Caenorhabditis elegans (C. elegans) was investigated. Survival assays, health span assays, Enzyme-Linked Immunosorbent Assay (ELISA), Stress biomarker detection assays, Green Fluorescence Proteins (GFP), Real Time quantitative PCR (RT-qPCR) and C. elegans mutants were employed to investigate the stress buffering and longevity effect of AE. In the study, it was observed that AE supplementation improved health span and survival in both normal and stressed worms. Additionally, AE positively regulated stress hormones (cortisol, oxytocin, and dopamine) and decreased fat and reactive oxygen species (ROS) accumulation. Through the Insulin/IGF-1 signaling (IIS) pathway, AE enhanced the nuclear localization of DAF-16 and the expression of heat shock proteins and antioxidant genes in GFP-tagged worms and at messenger RNA levels. Finally, AE failed to increase the survival of daf-16, daf-2, skn-1 and hsf-1 loss-of-function mutants, confirming the involvement of the IIS pathway. Evidently, AE supplementation relieves stress and enhances longevity. Thus, amber may be a potent nutraceutical for stress relief.

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“…Commonly, amber is used in jewelry or ornaments, but in Russia and Scandinavia, it has been used as an antipyretic and anti-inflammatory agent, as well as incense because of its unique sweet smell that is emanated when it is heated. Recently, amber has been shown the potential to prevent Alzheimer’s disease [ 9 ], and it has been proved to reduce the inflammation reaction in our lab [ 10 ]. Therefore, we hope to further study whether amber can regulate lipid metabolism inside adipocytes, and to investigate the mechanism.…”

Section: Introductionmentioning

confidence: 99%

Amber Extract Reduces Lipid Content in Mature 3T3-L1 Adipocytes by Activating the Lipolysis Pathway

Sogo

Zhou

Haeiwa³

et al. 2021

Molecules

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Amber—the fossilized resin of trees—is rich in terpenoids and rosin acids. The physiological effects, such as antipyretic, sedative, and anti-inflammatory, were used in traditional medicine. This study aims to clarify the physiological effects of amber extract on lipid metabolism in mouse 3T3-L1 cells. Mature adipocytes are used to evaluate the effect of amber extract on lipolysis by measuring the triglyceride content, glucose uptake, glycerol release, and lipolysis-related gene expression. Our results show that the amount of triacylglycerol, which is stored in lipid droplets in mature adipocytes, decreases following 96 h of treatment with different concentrations of amber extract. Amber extract treatment also decreases glucose uptake and increases the release of glycerol from the cells. Moreover, amber extract increases the expression of lipolysis-related genes encoding perilipin and hormone-sensitive lipase (HSL) and promotes the activity of HSL (by increasing HSL phosphorylation). Amber extract treatment also regulates the expression of other adipocytokines in mature adipocytes, such as adiponectin and leptin. Overall, our results indicate that amber extract increases the expression of lipolysis-related genes to induce lipolysis in 3T3-L1 cells, highlighting its potential for treating various obesity-related diseases.

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Anti-inflammatory activities of amber extract in lipopolysaccharide-induced RAW 264.7 macrophages

Cited by 26 publications

References 25 publications

Protective Effect of Amber Extract on Human Dopaminergic Cells against 6-Hydroxydopamine-Induced Neurotoxicity

Protective Effect of Amber Extract on Human Dopaminergic Cells against 6-Hydroxydopamine-Induced Neurotoxicity

Stress Buffering and Longevity Effects of Amber Extract on Caenorhabditis elegans (C. elegans)

Amber Extract Reduces Lipid Content in Mature 3T3-L1 Adipocytes by Activating the Lipolysis Pathway

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