Anti-inflammatory action of hydroalcoholic extract, dichloromethane fraction and steroid α-spinasterol from Polygala sabulosa in LPS-induced peritonitis in mice

Borges, Fábio Rodrigo Mesquita; Silva, Morgana D.; Córdova, Marina Machado; Schambach, Tiago R.; Pizzolatti, Moacir Geraldo; Santos, Adair R.S.

doi:10.1016/j.jep.2013.10.009

Cited by 47 publications

(30 citation statements)

References 38 publications

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“…As shown in a previous study, α-spinasterol displaces [ 3 H] resiniferatoxin from the transient receptor potential vanilloid 1 receptor (TRPV1), a potential anxiety factor, in spinal cord membranes, suggesting that its anxiolytic-like effects may be mediated through inhibition of the TRPV1 receptor 59, 60 . Meanwhile, α-spinasterol treatments directly and completely inhibit the production or release of pro-inflammatory cytokines, such as TNF-α and IL-1β, in mice injected with lipopolysaccharide (LPS) 61 . Mice administered ferulic acid showed a greater duration of time spent in the open arm and a lower number of entries in the closed arm of the EPM, indicating a significant anxiolytic effect 62 .…”

Section: Discussionmentioning

confidence: 99%

Xiaoyaosan exerts anxiolytic-like effects by down-regulating the TNF-α/JAK2-STAT3 pathway in the rat hippocampus

Jiang

et al. 2017

Sci Rep

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Although the anxiolytic-like effects of Xiaoyaosan, a Chinese herbal formula, have been described in many previous studies, its underlying mechanism remains undefined. The cytokine tumour necrosis factor-α (TNF-α) and its closely associated janus kinase 2 (JAK2)-signal transducer and activator of transcription (STAT3) signalling pathway regulate the neuro-inflammatory response in the brain, thus participating in the development of anxiety. Our purpose was to investigate whether the anxiolytic-like effects of Xiaoyaosan are related to the TNF-α/JAK2-STAT3 pathway in the hippocampus. We examined the effects of Xiaoyaosan on behaviours exhibited in the elevated plus maze test, open field test and novelty-suppressed feeding test as well as hippocampal neuron damage and changes in the TNF-α/JAK2-STAT3 pathway in a rat model of chronic immobilization stress (CIS)-induced anxiety. Xiaoyaosan exerts anxiolytic-like effects on CIS-induced anxiety, with a significant alleviation of anxiety-like behaviours, an attenuation of hippocampal neuron damage, and a reversal of the activation of the TNF-α/JAK2-STAT3 pathway in the hippocampus that are similar to the effects of the JAK2 antagonist AG490. However, Xiaoyaosan and AG490 failed to effectively regulate apoptosis-related factors, including Bax and Caspase-3. These results suggest that Xiaoyaosan attenuates stress-induced anxiety behaviours by down-regulating the TNF-α/JAK2-STAT3 pathway in the rat hippocampus.

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Section: Discussionmentioning

confidence: 99%

Xiaoyaosan exerts anxiolytic-like effects by down-regulating the TNF-α/JAK2-STAT3 pathway in the rat hippocampus

Jiang

et al. 2017

Sci Rep

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“…Phytochemical studies of Polygala genus (Polygalaceae) characterized compounds as triterpenoids, anthraquinones, flavonoids, and saponins from Polygala Japonica [Wang et al, ], xanthones from Polygala telephioides [Chang et al, ]; coumarin, styrylpyrones, tocoferol, α‐spinasterol, and β‐sitosterol from Polygala sabulosa [Borges et al, ]; and the effect of these compounds was reported as anti‐inflammatory. In a recent study, a large amount of isoflavones and flavonoids from P. molluginifolia were isolated and reported as anti‐inflammatory agents [Choi et al, ; Arruda‐Silva et al, ].…”

Section: Discussionmentioning

confidence: 99%

Bis‐Pyrano Prenyl Isoflavone Improves Glucose Homeostasis by Inhibiting Dipeptidyl Peptidase‐4 in Hyperglycemic Rats

Altenhofen

Luz

Frederico

et al. 2016

J of Cellular Biochemistry

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Isoflavones widely distributed in plants prevent diabetes. This study investigated the in vivo and in vitro effect of 3',4'-dihydroxy-6″,6″,6″',6″'-tetramethylbis(pyrano[2″,3″:5,6::2″',3″':7,8]isoflavone (bis-pyrano prenyl isoflavone) on glucose homeostasis in hyperglycemic rats. The ethyl acetate fraction from aerial parts of Polygala molluginifolia that contain isoflavones was assayed on glucose tolerance, on in vitro maltase activity and on protein glycation. The isoflavone bis-pyrano prenyl isolated from this fraction was investigated on glucose homeostasis. The in vivo action of the isoflavone exhibits an anti-hyperglycemic effect by improving glucose tolerance, augmenting the liver glycogen, inhibiting maltase activity, and stimulating glucagon-like peptide-1 (GLP-1) and insulin secretion. The in vitro isoflavone inhibits dipeptidyl peptidase-4 (DPP-4) activity since the glucose tolerance was improved in the presence of the isoflavone as much as sitagliptin, an inhibitor of DPP-4. However, the co-incubation with isoflavone and sitagliptin exhibited an additive anti-hyperglycemic action. The isoflavone increased the GLP-1 faster than the positive hyperglycemic group, which shows that the intestine is a potential target. Thus, to clarify the main site of action in which isoflavone improves glucose balance, the in vitro mechanism of action of this compound was tested in intestine using calcium influx as a trigger for the signal pathways for GLP-1 secretion. The isoflavone stimulates calcium influx in intestine and its mechanism involves voltage-dependent calcium channels, phospholipase C, protein kinase C, and stored calcium contributing for GLP-1 secretion. In conclusion, the isoflavone regulates glycaemia by acting mainly in a serum target, the DPP-4 inhibitor. Furthermore, the long-term effect of isoflavone prevents protein glycation. J. Cell. Biochem. 118: 92-103, 2017. © 2016 Wiley Periodicals, Inc.

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“…Spinasterol has been reported to show various biological properties, including anticarcinogenic, anti-inflammatory, antitumor, antiulcerogenic activities, and antinociceptive effects [19,21,[27][28][29][30]].…”

Section: Sterolsmentioning

confidence: 99%

“…Additionally, the cytotoxicity of A. dealbata lipophilic extracts has not been exploited so far, although a wide range of biological properties have been already reported for some of its constituents, such as the anti-inflammatory, antidiabetic, antiviral, and anticancer activities of lupenone [18] and anti-inflammatory, antiproliferative, and anticarcinogenic activities associated to spinasterol and 22,23-dihydrospinasterol [19][20][21][22], which were identified as the major sterols in A. dealbata bark and wood [15].…”

Section: Introductionmentioning

confidence: 99%

Characterization and Cytotoxicity Assessment of the Lipophilic Fractions of Different Morphological Parts of Acacia dealbata

Oliveira

Moreira

Resende

et al. 2020

IJMS

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Acacia dealbata biomass, either from forest exploitation or from the management of invasive species, can be a strategic topic, namely as a source of high-value compounds. In this sense, the present study aimed at the detailed characterization of the lipophilic components of different morphological parts of A. dealbata and the evaluation of their cytotoxicity in cells representative of different mammals' tissues. The chemical composition of lipophilic extracts from A. dealbata bark, wood and leaves was evaluated using gas chromatography-mass spectrometry (GC-MS). Terpenic compounds (representing 50.2%-68.4% of the total bark and leaves extracts, respectively) and sterols (60.5% of the total wood extract) were the main components of these extracts. Other constituents, such as fatty acids, long-chain aliphatic alcohols, monoglycerides, and aromatic compounds were also detected in the studied extracts. All the extracts showed low or no cytotoxicity in the different cells tested, demonstrating their safety profile and highlighting their potential to be used in nutraceutical or pharmaceutical applications. This study is therefore an important contribution to the valorization of A. dealbata, demonstrating the potential of this species as a source of high value lipophilic compounds.

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Anti-inflammatory action of hydroalcoholic extract, dichloromethane fraction and steroid α-spinasterol from Polygala sabulosa in LPS-induced peritonitis in mice

Cited by 47 publications

References 38 publications

Xiaoyaosan exerts anxiolytic-like effects by down-regulating the TNF-α/JAK2-STAT3 pathway in the rat hippocampus

Xiaoyaosan exerts anxiolytic-like effects by down-regulating the TNF-α/JAK2-STAT3 pathway in the rat hippocampus

Bis‐Pyrano Prenyl Isoflavone Improves Glucose Homeostasis by Inhibiting Dipeptidyl Peptidase‐4 in Hyperglycemic Rats

Characterization and Cytotoxicity Assessment of the Lipophilic Fractions of Different Morphological Parts of Acacia dealbata

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