Anti-inflammation effects of the total saponin fraction from Dioscorea nipponica Makino on rats with gouty arthritis by influencing MAPK signalling pathway

Abstract

Background: Dioscorea nipponica Makino is widely used in traditional Chinese medicine to treat gouty arthritis. Methods: Sixty male Wistar rats were divided into six groups: the normal group, model group, colchicine group (COL) and three total saponin groups (RDN) (high dose [160 mg/kg], middle dose [80 mg/kg] and low dose [40 mg/ kg]). HE staining was used to detect the histopathologic changes of the synovial tissue of joint. Immunohistochemical method was used to detect the protein expressions of P-38, p-P38, JNK, p-JNK, ERK1/2, p-ERK1/2, MEK1/2, p-MEK1/2, MKK4, p-MKK4, ICAM1, VCAM1, and PPARγ in the synovial tissue of joint. Realtime PCR and WB methods were used to detect the mRNA and protein expressions of PPARγ and AdipoR2 in the synovial tissue of joint. The contents of CXCL1 and ADP in the blood serum were measured by Elisa method. Results: Our study showed that RDN could improve the situation of the synovial tissue, reduce the protein expressions of MKK4, p-MEK1/2, p-JNK, p-ERK1/2, ICAM1. They could also decrease the content of CXCL1 and increase the content of ADP in the blood serum. Conclusion: RDN has good effect of anti-inflammation. This is in part realized by influencing MAPK signalling pathway. It provides a new visual angle to reveal the mechanism of RDN to treat GA.

Dioscorea nipponica Makino Relieves Ovalbumin-Induced Asthma in Mice through Regulating RKIP-Mediated Raf-1/MEK/MAPK/ERK Signaling Pathway

“…In this study, the activated Raf-1/MEK/MAPK/ERK pathway in asthma mice was also inhibited by DNM, indicating a potential action mechanism of DNM. Zhou et al have shown that the saponin fraction from DNM exerts anti-inflammation effects on gouty arthritis by inhibiting MAPK pathway [ 24 ]. To combine with the promoting role of DNM on RKIP, we suspect that DNM may upregulate RKIP to block the Raf-1/MEK/MAPK/ERK pathway, thereby alleviating asthma.…”

“…Ginkgolide B inhibits OVA-induced eosinophilia in lung tissues and mucus hypersecretion in the airway in a mouse model [ 23 ]. In addition, previous studies have also determined that DNM exhibits a great potential in the treatment of gouty arthritis via regulating MAPK pathway [ 24 , 25 ]. However, the action mechanisms of DNM in asthma involving the MAPK pathway are not entirely understood.…”

Natural Deep Eutectic Solvents for the Extraction of Bioactive Steroidal Saponins from Dioscoreae Nipponicae Rhizoma

“…Dioscoreae Nipponicae Rhizoma (DNR), the dried rhizome of Dioscorea nipponica Makino, is widely distributed in China, South Korea, and Russia, and has been used as a traditional herbal medicine for relieving cough and asthma, eliminating rheumatic aches, alleviating pain, and improving blood circulation [1]. Modern pharmacological studies have discovered that DNR possesses anti-tumor, anti-inflammatory, anti-diuretic, analgesic, anti-tussive, immunoregulatory, and cardiovascular properties [2][3][4][5][6]. Phytochemical studies disclosed that steroidal saponins, such as protodioscin, protogracillin, pseudoprotodioscin, and pseudoprotogracillin are the major bioactive constituents in DNR, which are mainly responsible for most of the pharmacological effects of this herbal medicine [7,8].…”

Dioscorea nipponica Makino Rhizome Extract and Its Active Compound Dioscin Protect against Neuroinflammation and Scopolamine-Induced Memory Deficits

“…Dioscorea nipponica has also been shown to promote muscle differentiation, restore muscle atrophy, and recover from injury by inhibiting the NF-κB pathway [ 16 ]. The total saponin fraction and phenolic compounds from Dioscorea nipponica extract have been shown as potential modulators of the MAPK signalling pathway in an inflammatory disease model [ 15 , 17 ]. However, the biological impact of Dioscorea nipponica in neuroinflammation progression or prevention is largely unknown.…”