Anti-IgM-Induced Growth Inhibition and Apoptosis Are Independent of Ornithine Decarboxylase in Ramos Cells☆

Lin, Chia-Yu; Zou, Huichao; Kaptein, John S.; Yen, Cindy F.; Kalunta, Cosmas I.; Nguyen, Tam; Park, E; Lad, Pramod M.

doi:10.1006/excr.1997.3794

Cited by 14 publications

(6 citation statements)

References 42 publications

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“…Again, the possibility exists that MGBG may have different effects on normal and tumor cells, consistent with the reports that low PAO activity may contribute to the relatively low level of apoptosis observed in tumor cells [42]. MGBG has recently been demonstrated to confer a dose-dependent protection against the damaging action of ethanol and other destructive agents on normal gastric mucosa of rats [43], whereas the MGBG-induced inhibition of polyamine synthesis results in growth inhibition (though without apoptosis) in Burkitt's lymphoma cells [44].…”

Section: Discussionsupporting

confidence: 68%

Administration of the antitumor drug mitoguazone protects normal thymocytes against spontaneous and etoposide-induced apoptosis

Ferioli¹,

Bottone

Soldani

et al. 2004

CMLS, Cell. Mol. Life Sci.

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The suggestion has been made that polyamines may be involved in the control of cell death, since exceedingly high or low levels induce apoptosis in different cell systems. For a deeper insight into the relationship between apoptosis and polyamine metabolism, we investigated in vitro the effect on rat thymocytes of mitoguazone (MGBG, which inhibits S-adenosylmethionine decarboxylase, i.e. a key enzyme in the polyamine biosynthetic pathway). Thymocytes were selected as an especially suitable model system, since they undergo spontaneous apoptosis in vivo and can be easily induced to apoptose in vitro by etoposide, used here as an apoptogenic agent. MGBG protected thymocytes from both spontaneous and drug-induced apoptosis, and this protective effect was associated with a decrease in polyamine oxidase activity and total polyamine levels.

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Section: Discussionsupporting

confidence: 68%

Administration of the antitumor drug mitoguazone protects normal thymocytes against spontaneous and etoposide-induced apoptosis

Ferioli¹,

Bottone

Soldani

et al. 2004

CMLS, Cell. Mol. Life Sci.

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“…Anti-IgM treatment induces growth inhibition in Ramos cells. 25 Therefore, the observed expression of cell cycle regulation genes is primarily due to their function in cellular differentiation.…”

Section: Cell Cycle Regulationmentioning

confidence: 99%

“…[37][38][39] Very little is known about the progression of apoptosis after the crosslinking of IgM. Apoptosis in Ramos cells begins only 4 h after the stimulation, 25,40 and reaches a maximum around 24 h. 38 Under certain conditions, non apoptotic cells that survive BCR stimulation show diminished expression of the surface molecules integrin VLA-4 and BCR after 24 h. 41 In our study, the a4 subunit of VLA-4, Iga, Igb and the C region of IgM are indeed downregulated 24 h after stimulation, indicating that only a fraction of Ramos B-cells become apoptotic during the experiment. The amount of apoptotic cells was small since no RNA degradation was detected.…”

Section: Cell Cycle Regulationmentioning

confidence: 99%

Stimulation-induced gene expression in Ramos B-cells

Ollila

Vihinen

2003

Genes Immun

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The development of adaptive immunity and responses to foreign molecules and organisms relies on the highly regulated production of hundreds of proteins. B-cell maturation, from committed progenitors to terminally differentiated plasma cells, is a multistep process that requires the ordered expression of a large number of genes. We studied anti-IgM-stimulated Ramos cells to explore genome-wide expression patterns in differentiating human B-cells. cDNA microarrays were used to measure changes in transcript levels over several days. A large set of genes (B1500) showed significantly altered expression at one or more time points. The expression profiles were used to construct gene clusters that were then characterized further with respect to the functions of the encoded proteins. Several groups of genes relevant to B-cells were analyzed in detail including early response genes and genes related to transcription, apoptosis and cell cycle regulation. Extensive bioinformatics analyses were conducted to identify the genes/proteins and to study functions and pathways involving B-cells. The results pave the way for understanding the development of humoral immunity, and provide new candidate genes and targets for research and drug development.

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“…Noda et al (18) showed that ischemia-reperfusion damage in rat intestinal mucosa resulted in elevated ODC activity accompanied by DNA fragmentation. Inhibition of ODC activity by DFMO did not affect DNA fragmentation, indicating that apoptosis was independent of ODC activity (19). Penning et al (20) also showed that apoptosis stimulated by TNF-␣ was independent of ODC activity per se but was dependent on intracellular levels of spermine.…”

mentioning

confidence: 96%

Protein Phosphatase 2A Regulates Apoptosis in Intestinal Epithelial Cells

Ray

Bhattacharya

Johnson

2005

Journal of Biological Chemistry

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Polyamine depletion prevents apoptosis by increasing serine/threonine phosphorylation leading to either inactivation or activation of pro-and anti-apoptotic proteins, respectively. Despite evidence that protein kinases are regulators of apoptosis, a specific role for protein phosphatases in regulating cell survival has not been established. In this study, we show that polyamine depletion inhibits serine/threonine phosphatase 2A ( These results indicate that polyamines regulate PP2A activity, and inhibition of PP2A in response to polyamine depletion increases steady state levels of Bad and Bcl-2 proteins and their phosphorylation and thereby prevents cytochrome c release, caspase-9, and caspase-3 activation.

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Anti-IgM-Induced Growth Inhibition and Apoptosis Are Independent of Ornithine Decarboxylase in Ramos Cells☆

Cited by 14 publications

References 42 publications

Administration of the antitumor drug mitoguazone protects normal thymocytes against spontaneous and etoposide-induced apoptosis

Administration of the antitumor drug mitoguazone protects normal thymocytes against spontaneous and etoposide-induced apoptosis

Stimulation-induced gene expression in Ramos B-cells

Protein Phosphatase 2A Regulates Apoptosis in Intestinal Epithelial Cells

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