Anti-Idiotypic Nanobody Alkaline Phosphatase Fusion Protein-Triggered On–Off–On Fluorescence Immunosensor for Aflatoxin in Cereals
Gege Yu,
Jiamin Wang,
Yao Zhang
et al.
Abstract:Nanobodies (Nbs) are widely used in immunoassays with the advantages of small size and high stability. Here, the nanobody employed as the surrogate of aflatoxin antigen and the recognition mechanism of antiaflatoxin mAb with nanobody was studied by molecular modeling, which verified the feasibility of Nbs as antigen substitutes. On this basis, a nanobody-alkaline phosphatase fusion protein (Nb-AP) was constructed, and a highly sensitive "on−off−on" fluorescent immunosensor (OFO-FL immunosensor) based on the ca… Show more
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