Anti-<i>Candida albicans</i>effectiveness of citral and investigation of mode of action

Lima, Igara Oliveira; Nóbrega, Fernanda de Medeiros; Oliveira, Wylly Araújo de; Lima, Edeltrudes de Oliveira; Menezes, Everardo Albuquerque; Cunha, Francisco Afrânio; Diniz, Margareth de Fátima Formiga Melo

doi:10.3109/13880209.2012.694893

Cited by 34 publications

(32 citation statements)

References 31 publications

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“…These results are in agreement with those reported by Lima et al [44] who have also described the antifungal activity of this volatile compound against C. albicans .…”

Section: Resultssupporting

confidence: 93%

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Evaluation of Antifungal Activity and Mechanism of Action of Citral against Candida albicans

Leite

Bezerra

Sousa

et al. 2014

Evidence-Based Complementary and Alternative Medicine

161

113

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Candida albicans is a yeast that commensally inhabits the human body and can cause opportunistic or pathogenic infections. Objective. To investigate the antifungal activity of citral against C. albicans. Methodology. The minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) were determined by the broth microdilution techniques. We also investigated possible citral action on cell walls (0.8 M sorbitol), cell membranes (citral to ergosterol binding), the time-kill curve, and biological activity on the yeast's morphology. Results. The MIC and MFC of citral were, respectively, 64 µg/mL and 256 µg/mL. Involvement with the cell wall and ergosterol binding were excluded as possible mechanisms of action. In the morphological interference assay, it was observed that the product inhibited pseudohyphae and chlamydoconidia formation. The MIC and the MFC of citral required only 4 hours of exposure to effectively kill 99.9% of the inoculum. Conclusion. Citral showed in vitro antifungal potential against strains of C. albicans. Citral's mechanism of action does not involve the cell wall or ergosterol, and further study is needed to completely describe its effects before being used in the future as a component of new antifungals.

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“…These results are in agreement with those reported by Lima et al [44] who have also described the antifungal activity of this volatile compound against C. albicans .…”

Section: Resultssupporting

confidence: 93%

“…However, citral exhibited excellent activity, even more effective than previously reported, where the compound exhibited antifungal activity in concentrations greater than 256 μ g/mL against C. albicans [24, 42–44]. …”

Section: Resultsmentioning

confidence: 89%

Evaluation of Antifungal Activity and Mechanism of Action of Citral against Candida albicans

Leite

Bezerra

Sousa

et al. 2014

Evidence-Based Complementary and Alternative Medicine

161

113

View full text Add to dashboard Cite

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“…The high incidence of infections by C. albicans and the emergence of resistance to the antifungal drugs accentuates the need of studying new sources of drugs, such as natural products and their phytoconstituents (LIMA et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Study of the antifungal potential of (r)-(+)-citronellal and its association with therapeutic agents used in the treatment of vulvovaginal candidiasis

Medeiros¹,

Silva²,

Pérez³

et al. 2017

Biosci. J.

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ABSTRACT:Vulvovaginal candidiasis (VVC) is a common fungal infection that affects healthy women of all ages. At least 75% of women will develop one or more infections once during their lifetime, with 6 to 9% of those individuals developing recurrent infections. In view of this context, this study sought to evaluate the antifungal potential of the isolated (R)-(+)-citronellal [(R)-(+)-CT] and associated to therapeutic agents of clinical importance. The enantiomer was solubilized in tween 80 and dimethylsulfoxide (DMSO). Posteriorly diluted in sterile distilled water up to the concentration of 2048µg/mL. The minimum inhibitory concentration (MIC) of the product was determined by microdilution in RPMI-1640 obtaining dilutions of 1024-4µg/mL. The minimum fungicidal concentration (MFC) was determined by the Sabouraud dextrose agar (SDA) depletion technique from aliquots of 1µL of the MIC, MIC × 2 and MIC × 4. The MIC and the MFC values of (R)-(+)-CT for 90% of the C. albicans strains were 16 and 32µg/mL respectively. In the susceptibility test, C. albicans presented a high resistance to fluconazole and to itraconazole, 12 (92.30%) of the strains. However, for ketoconazole and miconazole the resistance was of 4 (30.76%) and 3 (23.07%) of the strains respectively. In the combination testing of the (R)-(+)-CT with ketoconazole and miconazole, the resistance was completely reverted. For fluconazole and itraconazole, the resistance was reverted in 9 (75%) and 7 (58.33%) of the strains respectively. The (R)-(+)-CT presented fungicide activity with MFC of MIC × 2. When in combination with ketoconazole, fluconazole, itraconazole and miconazole increased the inhibition zones of these antifungal drugs, reducing the resistance against C. albicans.

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“…The outstanding fungicidal activity of citral (mainly against LT, in this work), has been discussed by several authors, who relate its fungicidal activity to the high capacity of receiving electrons from the fungus cell by a charge transfer with one electron donor present in the cell, resulting in the fungus death (KURITA et al, 1981). Another probable mode of action of citral is related to the cell wall synthesis or ergosterol (LIMA et al, 2012). Guimarães et al (2011) reported similar results on the inhibition power of citral by investigating the fungitoxic effects of the essential oil of lemongrass and its major compound (citral) on the phytopathogens Fusarium oxysporum cubense, Colletotrichum gloeosporioides, Bipolaris sp., and Alternaria alternata.…”

Section: Resultsmentioning

confidence: 77%

Activity of essential oils of Lippia alba chemotypes and their major monoterpenes against phytopathogenic fungi

Peixoto¹,

Blank²,

Arrigoni-Blank³

et al. 2018

Biosci. J.

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This work aimed to evaluate the in vitro antifungal activity of the essential oils of L. alba belonging to the carvone chemotype (LA-13 and LA-57) and the citral chemotype (LA-10, LA-29, and LA-44); the carvone enantiomers (R)-(-)-carvone and (S)-(+)-carvone; and citral on phytopathogenic fungi Lasiodiplodia theobromae (LT), Fusarium pallidoroseum (FP) and Fusarium solani (FS). Concentrations of 0.01; 0.05; 0.1; 0.2; 0.3; 0.5 and 1.0 mL/100 mL were tested, and the percentage of mycelial growth inhibition (MGI) was calculated after 96h in relation to the control. Minimal Inhibitory Concentrations (MIC) and Minimal Fungicide Concentrations (MFC) were obtained for essential oils and compounds. From the concentration of 0.2 mL/100 mL, all the accessions and carvone enantiomers were effective against the fungus LT, except the accession LA-44, for which the maximum inhibition occurred from the concentration of 0.3 mL/100 mL. Citral was the most effective compound against LT, with 100% of MGI from the concentration of 0.05 mL /100 mL. All accessions and enantiomers caused 100% of MGI against FP fungus from the concentration of 0.2 mL/100 mL. Once again, citral stood out by providing the same result as the other treatments from the concentration of 0.1 mL/100 mL. Considering the fungus FS, carvone enantiomers and citral caused 100% of MGI from the concentration of 0.1 mL/100 mL while all accessions caused 100% of MGI from the concentration of 0.2 mL/100 mL. Citral and carvone enantiomers presented the lowest MIC values (0.1 mL/100 mL) against FS fungus. The MIC of citral for LT and FP were not determined at the concentrations tested. (R)-(-)-carvone enantiomer presented the lowest MIC (0.1 mL/100 mL) for the LT fungus. Most of the other accessions presented MIC of 0.2 mL/100 mL for the three fungi. In relation to the minimum fungicidal concentration (MFC), citral stood out with values from 0.05 mL/100 mL (LT). Citral and carvone presented the same MFC for FS (0.2 mL / 100 mL). The other accessions showed MFC values from 0.3 mL/100 mL for the three fungi. Essential oils of L. alba accessions, carvone enantiomers, and citral were efficient in phytopathogen control and could be considered as an alternative to fungicides for presenting inhibitory and fungicidal effect against these microorganisms at low concentrations.

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Anti-Candida albicanseffectiveness of citral and investigation of mode of action

Cited by 34 publications

References 31 publications

Evaluation of Antifungal Activity and Mechanism of Action of Citral against Candida albicans

Evaluation of Antifungal Activity and Mechanism of Action of Citral against Candida albicans

Study of the antifungal potential of (r)-(+)-citronellal and its association with therapeutic agents used in the treatment of vulvovaginal candidiasis

Activity of essential oils of Lippia alba chemotypes and their major monoterpenes against phytopathogenic fungi

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