Anti-Hyperuricemic, Anti-Inflammatory and Analgesic Effects of Siegesbeckia orientalis L. Resulting from the Fraction with High Phenolic Content

Nguyen, Thuy-Duong Thi; Thương, Phương Thiện; Hwang, In Hyun; Hoang, Thi Kim Huyen; Nguyen, Minh Khoi; Nguyen, Hoang Anh; Na, MinKyun

doi:10.1186/s12906-017-1698-z

Cited by 41 publications

(30 citation statements)

References 29 publications

(40 reference statements)

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“…The antihyperuricemic activity of α-LA investigated using the potassium oxonate-induced hyperuricemia in the rat's model according to Haidari et al [25] and Nguyen et al [26] with modifications. Animals were fasted by withdrawing of food and water 2 h before drugs administration.…”

Section: Evaluation Of Antihyperuricemic Activitymentioning

confidence: 99%

XANTHINE OXIDASE INHIBITORY, ANTIHYPERURICEMIC, ANTI-INFLAMMATORY, ANTINOCICEPTIVE ACTIVITY OF Α-Lipoic ACID IN GOUTY ARTHRITIS MODEL

Hameed

Ramadhan

2018

Asian J Pharm Clin Res

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Objective: The current study was carried out to investigate the xanthine oxidase inhibitory, antihyperuricemic, anti-inflammatory, and antinociception activity of α-lipoic acid (LA) in gouty model of rats.Methods: Enzyme assay was done using bovine milk xanthine oxidase (XO). The XO inhibitory activity in vitro was performed using different doses of α-LA, and the degree of XO inhibition was expressed as IC 50 . The antihyperuricemic activity of α-LA was tested in the potassium oxonate-induced hyperuricemic rats for 7 consecutive days of oral treatment of 10, 25, and 50 mg/kg doses. The anti-inflammatory activity was achieved through monosodium urate crystal-induced inflammation hind paw model in mice. The antinociception effects of α-LA were explored using both of writhings induced by acetic acid test and hot plate test. Results:The study results revealed that the α-LA has a potent activity of XO inhibition with IC 50 = 2.93 µg/mL. Furthermore, these results showed that all doses of α-LA were able to significant reduce of each the following: Serum uric acid levels in the hyperuricemic rats, hind paw thickness of mice at all periods of time assessment, and writhings number in the acetic acid-induced writhing test, while, the same doses significantly increased the reaction latency in mice at hot plate test. Conclusion:The α-LA showed a significant effect on the evaluated models, and therefore it may be a promising agent for the treatment of gout since it possesses XO inhibitory, antihyperuricemic, anti-inflammatory, and antinociception activity.

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Section: Evaluation Of Antihyperuricemic Activitymentioning

confidence: 99%

XANTHINE OXIDASE INHIBITORY, ANTIHYPERURICEMIC, ANTI-INFLAMMATORY, ANTINOCICEPTIVE ACTIVITY OF Α-Lipoic ACID IN GOUTY ARTHRITIS MODEL

Hameed

Ramadhan

2018

Asian J Pharm Clin Res

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“…In the 2015 edition of Chinese Pharmacopeia, it is recorded that the aerial parts of three Siegesbeckia genus plants Siegesbeckia pubescens Makino, S. orientalis L. and S. glabrescens Makino are used as HS (3). In the clinic, HS is widely prescribed to treat inflammatory diseases such as furuncle, arthritis and gout (4). Some preparations made solely from this herb are commercially available (e.g.…”

Section: Introductionmentioning

confidence: 99%

“…Pharmacological studies have demonstrated that HS possesses anti-inflammatory effects in animal models, such as xylene-induced ear edema in mice; cottonball-induced granuloma (6), collagenase-induced paw edema (7) and urate-induced synovitis in rats (4); as well as rheumatoid arthritis induced by mixed arthrogen monoclonal antibody in mice (8). In vitro studies have shown that HS inhibits inflammatory mediators by suppressing the MAPK-and NF-κB-pathways in LPSstimulated RAW264.7 cells (9).…”

Section: Introductionmentioning

confidence: 99%

An ethanolic extract of the aerial part of <i>Siegesbeckia orientalis </i>L. inhibits the production of inflammatory mediators regulated by AP-1, NF-κB and IRF3 in LPS-stimulated RAW 264.7 cells

Guo

Zhang

Cheng

et al. 2018

BST

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Herba Siegesbeckiae (HS, the dried aerial part of Siegesbeckia orientalis L.) is a commonly used traditional Chinese medicinal herb for treating inflammatory diseases. HS has been reported to exert anti-inflammatory effects by inhibiting the MAPKs and NF-κB pathways, the downstream effectors of TLR4 signalling. This study aims to further investigate the involvement of TLR4 signalling cascades in the effects of an ethanolic extract of HS (HS for short) on inflammatory mediators in murine macrophages. HS was extracted using 50% ethanol. Lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages were used as the cell model. ELISA was used to detect cytokine/chemokine secretion. Real time-PCR and immunoblotting were used to examine mRNA and protein levels, respectively. We observed that HS dose-dependently inhibited the secretion of PGE, MCP-1, MIP-1α and RANTES, and down-regulated mRNA levels of iNOS, COX-2, IL-1β, IL-6, TNF-α, mPGES-1, MCP-1, MIP-1α and RANTES in LPS-stimulated RAW264.7 cells. HS did not affect the protein levels of TAK1, TBK1, PI3K, Akt, IKK, c-Jun, c-Fos and IRF3, while, dose-dependently decreased levels of their phosphorylated forms. The protein levels of IRAK1 and IRAK4 were upregulated, while those of TRAF6 and TRAF3 were downregulated by HS. Moreover, the nuclear protein levels of AP-1, NF-κB and IRF3 were dose-dependently decreased by HS. These results indicate that suppression of the IRAK4/MAPKs/AP-1, IRAK4/MAPKs/NF-κB, IRAK4/PI3K/NF-κB and TRAF3/TBK1/IRF3 pathways is associated with the inhibitory effects of HS on inflammatory mediators in LPS-stimulated RAW264.7 cells. This study provides a pharmacological basis for the clinical application of this herb in the treatment of inflammatory disorders.

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“…Its ethanol extract could suppress the growth and metastasis of endometrial cancer cells [ 30 , 31 ]. Additionally, S. orientalis extracts possess significant anti-inflammatory, anti-hyperuricemic and analgesic activities [ 32 , 33 ]. However, to our best knowledge, the effects of S. orientalis extracts on metabolic syndrome, such as Type 2 diabetes-linked hyperglycemia and related cardiovascular complications, have not yet been reported in the literature.…”

Section: Introductionmentioning

confidence: 99%

Inhibitory Effects of Siegesbeckia orientalis Extracts on Advanced Glycation End Product Formation and Key Enzymes Related to Metabolic Syndrome

et al. 2017

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Metabolic syndrome typically includes Type 2 diabetes associated with hyperglycemia, central obesity, dyslipidemia and hypertension. It is highly related to oxidative stress, formation of advanced glycated end products (AGEs) and key enzymes, such as carbohydrate digesting enzymes like pancreatic α-amylase and intestinal α-glucosidase, pancreatic lipase and angiotensin I-converting enzyme (ACE). This study used an in vitro approach to assess the potential of four extracts of Siegesbeckia orientalis linne on key enzymes relevant to metabolic syndrome. In this research, S. orientailis was firstly extracted by ethanol. The ethanol extract (SE) was then partitioned sequentially with hexane, ethyl acetate and methanol, and these extracts were named SE-Hex, SE-EA and SE-MeOH, respectively. The experimental results showed that SE-EA had the highest total phenolic content (TPC, 76.9 ± 1.8 mg/g) and the total flavonoids content (TFC, 5.3 ± 0.3 mg/g). This extract exhibited the most significant antioxidant activities, including DPPH radical-scavenging capacity (IC50 = 161.8 ± 2.4 μg/mL), ABTS radical-scavenging capacity (IC50 = 13.9 ± 1.5 μg/mL) and reducing power. For anti-glycation activities, SE-EA showed the best results in the inhibition of AGEs, as well as inhibitory activities against α-glucosidase (IC50 = 362.3 ± 9.2 μg/mL) and α-amylase (IC50 = 119.0 ± 17.7 μg/mL). For anti-obesity activities, SE-EA indicated the highest suppression effect on pancreatic lipase (IC50 = 3.67 ± 0.52 mg/mL). Finally, for anti-hypertension activity, SE-EA also demonstrated the strongest inhibitory activity on ACE (IC50 = 626.6 ± 15.0 μg/mL). Close relationships were observed among the parameters of TPC, antioxidant activities, inhibitory activities on α-amylase, α-glucosidase, lipase and ACE (R > 0.9). Moderate correlations were found among the parameters of TFC, antioxidant activities, and suppression of dicarbonyl compounds formation (R = 0.5–0.9). Taken together these in vitro studies reveal the therapeutic potential of SE-EA extract in the prevention and treatment of metabolic disorders.

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Anti-Hyperuricemic, Anti-Inflammatory and Analgesic Effects of Siegesbeckia orientalis L. Resulting from the Fraction with High Phenolic Content

Cited by 41 publications

References 29 publications

XANTHINE OXIDASE INHIBITORY, ANTIHYPERURICEMIC, ANTI-INFLAMMATORY, ANTINOCICEPTIVE ACTIVITY OF Α-Lipoic ACID IN GOUTY ARTHRITIS MODEL

XANTHINE OXIDASE INHIBITORY, ANTIHYPERURICEMIC, ANTI-INFLAMMATORY, ANTINOCICEPTIVE ACTIVITY OF Α-Lipoic ACID IN GOUTY ARTHRITIS MODEL

An ethanolic extract of the aerial part of <i>Siegesbeckia orientalis </i>L. inhibits the production of inflammatory mediators regulated by AP-1, NF-κB and IRF3 in LPS-stimulated RAW 264.7 cells

Inhibitory Effects of Siegesbeckia orientalis Extracts on Advanced Glycation End Product Formation and Key Enzymes Related to Metabolic Syndrome

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