Anti‐Hyperglycemic Properties of Crude Extract and Triterpenes  from <i>Poria cocos</i>

Li, Tzu-Hsuan; Hou, Chia-Chung; Chang, Ching‐Cheng; Yang, Wu-Chang

doi:10.1155/2011/128402

Cited by 78 publications

(44 citation statements)

References 30 publications

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“…Another study to support the results is the hypoglycemic effect of terpenes 25 . Flavonoids and Tannins would reduce plasma cholesterol levels by inhibiting LDL oxidation 26 .…”

Section: Fig 9: Linear Regression Of the Percent Adaptogenic Activitmentioning

confidence: 73%

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2017

IJPSR

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ABSTRACT:Artemisia vulgaris leaves are known to have anectodatal evidence of antioxidant activity which can be of use as an anti -stress remedy. However, this claim is yet to be proven and no available data on its adaptogenic potential hence, this study aimed to determine the adaptogenic potential of Artemisia vulgaris leaf extract on male albino rats using acute restraint stress. Six male albino rats were used as test animals and were subdivided into the following: control group, untreated normal group, negative group, and three doses of the test solution [500mg/200g rat (T1), 250mg/200g rat (T2), and 150 mg/200g rat (T3)]. T3 rats exhibited the highest adaptogenic activity of -3,111.11% based on cholesterol levels and -1916.67% based on glucose levels. Moreover, glucose levels of T2 and T1 rats were -345.41% and -6.60%, respectively while for their cholesterol levels, T2 exhibited -277.78% and T1 exhibited -90.05%. The median effective dose of the test suspension based on cholesterol levels and glucose levels is 169.70 mg/200g rat and 151.81mg/200g rats, respectively. From this study, Artemisia vulgaris leaf extract has the potential protective effect against stress. Flavanoids, tannins and saponins in the leaf extract of Artemisia vulgaris were attributed for its adaptogenic activity.

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“…Another study to support the results is the hypoglycemic effect of terpenes 25 . Flavonoids and Tannins would reduce plasma cholesterol levels by inhibiting LDL oxidation 26 .…”

Section: Fig 9: Linear Regression Of the Percent Adaptogenic Activitmentioning

confidence: 73%

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2017

IJPSR

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“…Ion detection was performed in multiple reaction monitoring (MRM) mode wherein the m/z 527.4 527. 4 We assessed potential analyte interference in the LC peak region with six different blank rat plasma samples using the proposed extraction procedure and LC-MS/MS conditions. Quality control (QC) stock solutions of PA (1 mg/mL) and GA (1 mg/mL) were prepared by dissolving the standards in acetonitrile, respectively.…”

Section: Lc-ms/ms Instrument and Conditionsmentioning

confidence: 99%

“…1) P. cocos alone or in combination with other herbs is often used to treat diabetes as well as other disorders. [2][3][4] The main ingredients of this herbal medicine include a group of triterpenoid compounds. Pachymic acid (PA) is one of the major components in P. cocos and plays an important role in various pharmacological effects.…”

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confidence: 99%

Determination and Pharmacokinetic Study of Pachymic Acid by LC-MS/MS

Wang

Liang

2015

Biological & Pharmaceutical Bulletin

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Poria cocos is a well-known medicinal plant widely used in China and other East Asian countries owing to its various therapeutic effects. Pachymic acid (PA) is a bioactive lanostrane-type triterpenoid from Poria cocos. In this paper, a method of high-performance liquid chromatographic (LC) coupled with triple quadrupole tandem mass spectrometry (QQQ-MS/MS) was developed and validated to investigate the concentration of PA in rat plasma. Samples were prepared by a liquid-liquid extraction, and chromatographic separation was achieved with a Phenomenex Gemini C 18 column (50 mm 2.0 mm i.d.) using a mobile phase consisting of acetonitrile and 0.05% formic acid (85 : 15, v/v) at a flow rate of 0.3 mL/min. The detection was performed on an Applied Bio-Systems API 4000 MS/MS with an electrospray ionization (ESI) inlet in negative multiple reaction monitoring (MRM) mode. Standard curves of samples in plasma were linear (R 2 0.9948) over the concentration range of 5-500 ng/mL, and acceptable accuracy and precision were achieved. The lower limit of quantification and detection were 5 and 0.5 ng/mL, respectively. The method was used successfully to study the pharmacokinetics of PA in rats for oral administration. The main pharmacokinetic parameters of elimination half-life (t 1/2 ), area under the plasma concentration-time curve from time zero to infinity (AUC 0→∞ ), plasma clearance (CL), and apparent volume of distribution (V d ) for the PA group were 4.96 1.33 h, 1466.9 361.7 ng·h/mL, 6.82 1.73 L/h, and 48.85 9.47 L, respectively. This LC-MS/MS method can be developed further for clinical investigation of PA-containing products. Key words Poria cocos; pachymic acid; quantitation; rat plasma; pharmacokinetics; LC-MS/MSBecause of their low toxicities and high levels of effectiveness against many diseases, traditional Chinese medicines (TCMs) have played an important role in the health of the Chinese people for thousands of years. In addition, they have gained ever-increasing global attention owing to the desirability of their bioactive compounds for the discovery of novel drug candidates. Poria cocos, which is also known as Fu Ling, is often used in TCM for its diuretic and sedative effects. 1) P. cocos alone or in combination with other herbs is often used to treat diabetes as well as other disorders. 2-4)The main ingredients of this herbal medicine include a group of triterpenoid compounds. Pachymic acid (PA) is one of the major components in P. cocos and plays an important role in various pharmacological effects. For example, PA has shown anti-inflammatory, 5)anti-apoptotic, and anti-immunologic rejection effects.6) In addition, it has exhibited cytotoxicity against a human colon carcinoma cell line.1,7) Furthermore, PA has been shown to induce apoptosis of human prostate cancer cells by reducing prostaglandin synthesis and AKT activity, 8,9) to stimulate glucose uptake, 10) and to inhibit the phospholipase A2 family of arachidonic acid-producing enzymes.11) Therefore, further pharmacodynamic and pharmacokinetic studie...

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“…Similarly, dehydrotrametenolic acid from Poria cocos Wolf, a mushroom used in traditional Chinese medicine to treat diabetes, was suggested to act as an insulin sensitiser through the action of PPARγ [68]. However, because it does not activate the PPARγ pathway, the enhanced insulin sensitivity induced by dehydrotrametenolic acid has been suggested to be irrespective of PPARγ [69]. It is important to remark that the lack of adipogenic activity and/or transcriptional activity of PPARγ in the presence of a compound must not be considered an evidence that their antidiabetic activity is not mediated by PPARγ.…”

Section: Natural Products That Modulate the Action Of Pparγmentioning

confidence: 99%