Anti-Human Olig2 Antibody as a Useful Immunohistochemical Marker of Normal Oligodendrocytes and Gliomas

Yokoo, Hideaki; Nobusawa, Sumihito; Takebayashi, Hirohide; Ikenaka, Kazuhiro; Isoda, Koji; Kamiya, Makoto; Sasaki, Atsushi; Hirato, Junko; Nakazato, Yoichi

doi:10.1016/s0002-9440(10)63730-3

Cited by 130 publications

(113 citation statements)

References 35 publications

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“…In contrast, more than 90% of these cells (Table 1) expressed the basic helix-loop-helix (bHLH) transcription factor Olig2 ( Figure 3E), a protein involved in lineage commitment of oligodendrocytes 31 and expressed by these cells at all stages of their differentiation. As described by others, 32 Olig2 staining localized exclusively in the nucleus, though there was clearly a range of staining intensities ( Figure 3E) with some (ϳ10%) ( Table 1) appearing negative ( Figure 3E, right). Thus, although they did not express markers of mature oligodendrocytes, a majority of the vacuolated cells appeared to belong to the oligodendrocyte lineage.…”

Section: Identification Of the Vacuolated Cellssupporting

confidence: 78%

Oligodendrocytes Are a Major Target of the Toxicity of Spongiogenic Murine Retroviruses

Clase

Dimcheff

Favara

et al. 2006

The American Journal of Pathology

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The neurovirulent retroviruses FrCasE and Moloney MLV-ts1 cause noninflammatory spongiform neurodegeneration in mice, manifested clinically by progressive spasticity and paralysis. Neurons have been thought to be the primary target of toxicity of these viruses. However the neurons themselves appear not to be infected, and the possible indirect mechanisms driving the neuronal toxicity have remained enigmatic. Here we have re-examined the cells that are damaged by these viruses, using lineage-specific markers. Surprisingly, these cells expressed the basic helix-loop-helix transcription factor Olig2, placing them in the oligodendrocyte lineage.

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Section: Identification Of the Vacuolated Cellssupporting

confidence: 78%

Oligodendrocytes Are a Major Target of the Toxicity of Spongiogenic Murine Retroviruses

Clase

Dimcheff

Favara

et al. 2006

The American Journal of Pathology

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“…The search for the expression levels of these markers in normal brain has highlighted that OLIG2 seems the marker with the lowest expression in adult normal brain, and the most specific of glial lineage. The data of the literature confirm that the expression of Olig2 in adult normal brain is restricted to progenitor cells and mature oligodendrocytes [42,59].…”

Section: Discussionmentioning

confidence: 53%

Identification of OLIG2 as the most specific glioblastoma stem cell marker starting from comparative analysis of data from similar DNA chip microarray platforms

et al. 2014

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Despite advances in surgical and adjuvant treatments, overall survival of glioblastoma (GBM) patients remains poor. The cancer stem cell concept suggests that a rare stem cell population, called glioma stem cells (GSCs), has high ability to self-renewal leading to recurrence in GBM. The identification of specific markers of GSCs would provide a powerful tool to detect and to characterise them in order to develop targeted therapies. We carried out a comparative analysis based on the identification of inter-study concordances to identify the genes that exhibit at best differential levels of expression between GSC-enriched cell cultures and differentiated tumour cell cultures from independent studies using DNA chip microarray technologies. We finally studied the protein expression of the marker we considered the most specific by immunohistochemistry and semi-quantitative analysis on a retrospective series of 18 GBMs. Of the selected studies, 32 genes were retained. Among them, eight genes were identified to be overexpressed in GSC-enriched cultures compared to differentiated tumour cell cultures. Finally, among the eight genes, oligodendrocyte lineage transcription factor 2 (OLIG2) was characterised by the most different expression level in the "GSC model" compared to the "differentiated tumour cells model". Our approach suggests that OLIG2 is the most specific GSC marker; additional investigations with careful considerations about methodology and strategies of validation are, however, mandatory.

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“…Although the expression pattern of OLIG2 is known to differ among gliomas (Lu et al, 2001;Marie et al, 2001;Yokoo et al, 2004), the biological function of this bHLH transcription factor in these tumors has been unclear. We have now established a human glioblastoma cell line, U12-1, in which the expression of OLIG2 is inducible.…”

Section: Discussionmentioning

confidence: 99%

“…Expression of the OLIG2 gene and protein has recently been found to be increased in human gliomas, primary tumors of the central nervous system, and OLIG2 has been proposed as a potential diagnostic marker for oligodendrogliomas (Lu et al, 2001;Marie et al, 2001;Yokoo et al, 2004). OLIG2 is highly expressed in the tumors of oligodendroglial lineage, which follow a relatively favorable clinical course.…”

Section: Introductionmentioning

confidence: 99%

A novel function of OLIG2 to suppress human glial tumor cell growth via p27Kip1 transactivation

Tabu

Ohnishi²,

Sunden

et al. 2006

Journal of Cell Science

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Kip1 gene promoter was essential for OLIG2-dependent activation of p27Kip1 gene transcription. Electrophoretic mobility shift assays confirmed that a nuclear extract of OLIG2-expressing U12-1 cells contained a protein complex that binds to the CTF site of the p27 Kip1 gene promoter. Furthermore, siRNA against p27 Kip1 rescued the OLIG2-mediated growth and DNA synthesis inhibition of U12-1 cells. These results indicate that OLIG2 suppresses the proliferation of U12-1 and that this effect is mediated by transactivation of the p27 Kip1 gene, and low expression of OLIG2 may be related to the malignant behavior of human glioblastoma.

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Anti-Human Olig2 Antibody as a Useful Immunohistochemical Marker of Normal Oligodendrocytes and Gliomas

Abstract: Olig2 is a recently identified transcription factor in-

Cited by 130 publications

References 35 publications

Oligodendrocytes Are a Major Target of the Toxicity of Spongiogenic Murine Retroviruses

Oligodendrocytes Are a Major Target of the Toxicity of Spongiogenic Murine Retroviruses

Identification of OLIG2 as the most specific glioblastoma stem cell marker starting from comparative analysis of data from similar DNA chip microarray platforms

A novel function of OLIG2 to suppress human glial tumor cell growth via p27Kip1 transactivation

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