Anti-HIV-1 seroreactivity and HIV transmission route[R1]

Bongertz, Vera; Guimarães, Monick Lindenmeyer; Soares-da-Costa, Mfg; Veloso, Valdiléa G.; Bastos, Francisco I.; Szwarcwald, Célia Landman; Derrico, Mônica; Telles, Paulo R.; Pilloto, J.H; João, Esaú; Morgado, Mariza G.

doi:10.1016/s0928-0197(98)00068-3

Cited by 9 publications

(10 citation statements)

References 24 publications

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“…Indeed, all F plasma samples recognized, for example, the V3B peptide, which is recognized by only 85% of B-GPG plasma specimens. As indicated before (Bongertz et al 1999), the Bbr plasma specimens showed much lower cross-reactivity with the different V3 peptides, and most high-titered reactions were observed against the homologous Bbr synthetic V3 peptide. Of greater interest would be if this extensive crossbinding activity was mirrored by extensive cross-neutralization, as this would be indicative of cross-protection, important for anti-HIV/AIDS vaccine research.…”

Section: Resultssupporting

confidence: 64%

“…The majority of the samples were obtained from male individuals (73.3%). Seroreactivity to synthetic V3 envelope peptides was carried out using reagents and methods described earlier (Bongertz et al 1999). Briefly, biotinylated decapentamer synthetic peptides corresponding to HIV-1 consensus amino acid sequences of HIV-1 genotypes A, B (including variant Bbr), C, D, E, and F (including an empirically chosen Brazilian sequence Fbr) were bound onto avidincoated immunoplates, incubated with duplicates of serially diluted sample and control plasma.…”

Section: Methodsmentioning

confidence: 99%

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Human immunodeficiency virus type 1 neutralization by plasma from B or F genotype infected individuals

Bongertz

Teixeira

Grinztejn

et al. 2005

Mem. Inst. Oswaldo Cruz

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Anti-human immunodeficiency virus type 1 (HIV-1) "binding antibodies" (antibodies capable of binding to synthetic peptides or proteins) occur throughout HIV-1 infection, are high-titered and highly cross-reactive, as confirmed in this study by analyzing plasma from B and F genotype HIV-1 infected individuals. Plasma from individuals infected with clade F HIV-1 displayed the most frequent cross-reactivity, in high titers, while Bbr plasma showed much higher specificity. Similarly, neutralization of a reference HIV-1 isolate (HIV-1 MN) was more frequently observed by plasma from F than B genotype infected individuals. No significant difference was seen in neutralization susceptibility of primary B, Bbr or F clade HIV-1 by plasma from individuals infected with the classical B (GPGR) or F HIV-1, but Bbr (GWGR) plasma were less likely to neutralize the F genotype primary HIV-1 isolates. The data indicate that both B and F genotype derived vaccines would be equally effective against B and F HIV-1 infection, with a slightly more probable effectiveness for F than B genotype. Although the Bbr variant appears to induce a much more specific humoral immune response, the susceptibility in neutralizing the Brazilian HIV-1 B genotype Bbr variant is similar to that observed with the classical B genotype HIV-1

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Section: Resultssupporting

confidence: 64%

Section: Methodsmentioning

confidence: 99%

Human immunodeficiency virus type 1 neutralization by plasma from B or F genotype infected individuals

Bongertz

Teixeira

Grinztejn

et al. 2005

Mem. Inst. Oswaldo Cruz

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“…Earlier studies carried out by our group showed a higher frequency of peptide recognition by IDU sera in comparison to sera from individuals belonging to other exposure categories (Bongertz et al 1999). Multiple logistic regression analyses confirmed the high importance of route of HIV-1 transmission in frequency of peptide recognition, even controlling for potential confounders.…”

supporting

confidence: 69%

Anti-human immunodeficiency virus-1 antibody titers in injection drug users compared to sexually infected individuals

Bongertz¹,

Ouverney²,

Teixeira³

et al. 2003

Mem. Inst. Oswaldo Cruz

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“…This could result from the differences in exposure to the virus, since in IDU the viral selection should be restricted to the susceptibility of mononuclear blood cells (8), possibly reinforced by the use of injected drugs, most importantly cocaine (24), as secondary immune stimulators (18,25). It is possible, therefore, that the route of transmission of the virus is at least as important in the induction of the humoral immune response as the health status of the individual, as demonstrated before (4).…”

Section: Discussionmentioning

confidence: 92%

“…Previous studies of HIV-1-positive Brazilian sera (4) showed an especially frequent reactivity against synthetic viral envelope peptides in IDU plasma compared to plasma from individuals belonging to other HIV-1 exposure categories (pregnant women, men that have sex with men, and sexually infected recent seroconverters). This observation indicates that the transmission mode of HIV-1 seems to be more predictive of the extent and titer of seroreactivity than the progress of the infection (indicated by the number of circulating CD4 T cells) or gender (5).…”

Section: Introductionmentioning

confidence: 91%

HIV-1 binding and neutralizing antibodies of injecting drug users

Ouverney

Teixeira

Jesus

et al. 2005

Braz J Med Biol Res

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Previous studies have demonstrated a stronger seroreactivity against some synthetic peptides responsible for inducing neutralizing antibodies in injecting drug users (IDU) compared to that of individuals sexually infected with HIV-1 (S), but the effectiveness in terms of the neutralizing ability of these antibodies has not been evaluated. Our objective was to study the humoral immune response of IDU by determining the specificity of their antibodies and the presence of neutralizing antibodies. The neutralization capacity against the HIV-1 isolate MN (genotype B), the primary HIV-1 isolate 95BRRJ021 (genotype F), and the seroreactivity with peptides known to induce neutralizing antibodies, from the V2 and V3 loops of different HIV-1 subtypes, were analyzed. Seroreactivity indicates that IDU plasma are more likely to recognize a broader range of peptides than S plasma, with significantly higher titers, especially of V3 peptides. Similar neutralization frequencies of the MN isolate were observed in plasma of the IDU (16/47) and S (20/60) groups in the 1:10 dilution. The neutralization of the 95BRRJ021 isolate was more frequently observed for plasma from the S group (15/23) than from the IDU group (15/47, P = 0.0108). No correlation between neutralization and seroreactivity with the peptides tested was observed. These results suggest that an important factor responsible for the extensive and broad humoral immune response observed in IDU is their infection route. There was very little difference in neutralizing antibody response between the IDU and S groups despite their differences in seroreactivity and health status.

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Anti-HIV-1 seroreactivity and HIV transmission route[R1]

Cited by 9 publications

References 24 publications

Human immunodeficiency virus type 1 neutralization by plasma from B or F genotype infected individuals

Human immunodeficiency virus type 1 neutralization by plasma from B or F genotype infected individuals

Anti-human immunodeficiency virus-1 antibody titers in injection drug users compared to sexually infected individuals

HIV-1 binding and neutralizing antibodies of injecting drug users

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