Anti-Helicobacter pylori Activity of the Methanolic Extract of Geum iranicum and its Main Compounds

Abstract

Geum iranicum Khatamsaz, belonging to the Rosaceae family, is an endemic plant of Iran. The methanol extract of the roots of this plant showed signifi cant activity against one of the clinical isolates of Helicobacter pylori which was resistant to metronidazole. The aim of this study was the isolation and evaluation of the major compounds of G. iranicum effective against H. pylori. The compounds were isolated using various chromatographic methods and identifi ed by spectroscopic data (1H and 13C NMR, HMQC, HMBC, EI-MS). An antimicrobial susceptibility test was performed employing the disk diffusion method against clinical isolates of H. pylori and a micro dilution method against several Gram-positive and Gram-negative bacteria; additionally the inhibition zone diameters (IZD) and minimum inhibitory concentrations (MIC) values were recorded. Nine compounds were isolated: two triterpenoids, uvaol and niga-ichigoside F1, three sterols, β-sitosterol, β-sitosteryl acetate, and β-sitosteryl linoleate, one phenyl propanoid, eugenol, one phenolic glycoside, gein, one fl avanol, (+)-catechin, and sucrose. The aqueous fraction, obtained by partitioning the MeOH extract with water and chloroform, was the most effective fraction of the extract against all clinical isolates of H. pylori. Further investigation of the isolated compounds showed that eugenol was effective against H. pylori but gein, diglycosidic eugenol, did not exhibit any activity against H. pylori. The subfraction D4 was the effective fraction which contained tannins. It appeared that tannins were probably the active compounds responsible for the anti-H. pylori activity of G. iranicum. The aqueous fraction showed a moderate inhibitory activity against both Gram-positive and Gram-negative bacteria. The MIC values indicated that Gram-positive bacteria including Staphylococcus aureus, Staphylococcus epidermidis, and Bacillus subtilis are more susceptible than Gram-neagative bacteria including Escherichia coli and Pseudomonas aeruginosa.

Biological activity and microscopic characterization of Lythrum salicaria L

“…2 equivalent to 6 × 10 8 cell/mL) and dried at room temperature for 10 min. Afterwards, the sterile blank disc (diameter: 6 mm) were impregnated in the plant extract (500 mg/mL) and placed on the plates, these plates were incubated at 37°C under microaerobic conditions and examined after 3–5 days [22]. Positive control plates included discs impregnated with amoxicillin (1 μg/mL) and the inhibition zone diameters (IZD) were recorded.…”

Quantification of sucrose in the root of Geum iranicum Khatamsaz

“…In a research the extract and essential oil of G. kokanicum showed antimicrobial activity and in another study a potent inhibitory effect on matrix metalloproteinase activity was reported from this plant (29,30). In our previous works we reported the presence of terpenoid, steroid, phenylpropanoid, tannin and sugar in the extract and high amount of eugenol in the essential oil of the roots of G. iranicum (4,31).…”

“…Few biological and phytochemical studies have been carried out on Geum species in Iran. Our previous work on phytochemical analysis of G. iranicum using column chromatography showed that the root was rich in sugars and sucrose was identified as a major sugar in this plant (4). Sugars as primary messengers in signal transduction regulate many important processes along the whole life cycle of the plant (5).…”

Terpenes From the Root of Salvia hypoleuca Benth

“…The mass data of the compounds 1, 2, 3, 6 and 7 have been previously reported [27,28]. The mass of other compounds are followed: Manool (4): EIMS (70eV) m/z : 290 [M] + (8), 272 (40), 204 (20), 257 (58), 189 (28), 137 (100), 121 (48), 95 (67).…”