Anti‐hapten response in vitro. Affinity differences in precursors of anti‐hapten antibody‐producing cells

Pasanen, V. J.; Virolainen, Martti

doi:10.1002/eji.1830010523

Cited by 10 publications

(7 citation statements)

References 7 publications

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“…The only likely explanation for this is that the AFCP are equipped with receptors having high affinity for antigen [45]. Such cells would therefore compete more efficiently for antigen than cells with low affinity receptors, or already secreted low affinity antibodies, which would exist in gradually decreasing antigen concentrations due t o catabolism.…”

Section: Discussionmentioning

confidence: 99%

Affinity of T and B lymphocyte receptors for hapten determinants

1973

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The affinity of anti-hapten antibodies, produced by single cells, was studied by hapten inhibition of plaque-forming cells using the local hemolysis in gel assay. The shift in affinity of anti-hapten antibodies, that occurs with time after immunization, was parallelled by an analogous shift, at the cellular level, with regard t o indirect plaque-forming cells (PFC). Thus, there was a gradual decrease of the hapten concentration which was needed t o suppress indirect plaque formation with time after immunization, indicating a gradual increase in the number of high affinity cells. No such shift was observed with cells causing direct plaque formation.Analogous studies were performed with hapten-specific antigen-binding cells of T and B origin by using hapten-inhbition of rosette-forming cells. Hapten-specific antigen-binding cells were present in both cell populations. Lymphocyte binding of haptenated red cells could be specifically inhibited by free hapten.It was found that antigen-binding B lymphoid cells became gradually more susceptible t o hapten inhibition with time after immunization, whereas no such change was observed in antigen-binding T lymphoid cells. Possible reasons for this discrepancy are discussed, as well as the implications for these findings regarding the specificity and structure of the T cell receptors.

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Section: Discussionmentioning

confidence: 99%

Affinity of T and B lymphocyte receptors for hapten determinants

1973

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“…We stress that in normal spleen cultures :~ Identical cultures contained either 3 )< 106 SRBC or 3 X 106 HRBC, All cultures were assayed on day 4 using the erythrocytes indicated in the plaque-forming assay and each figure represents the mean of duplicate cultures. (23)(24)(25) and in adult mice (26) (d) A background immune response to TNP is also found. When nude spleen cells are cultured with different concentrations of LPS and assayed at various times using HRB C or TNP-HRBC as the indicator in the plaque-forming assay, PFC specific for both HRBC and TNP can be distinguished.…”

Section: Effect Of Antigen On Lps-induced Immunementioning

confidence: 90%

The Use of Bacterial Lipopolysaccharides to Show That Two Signals Are Required for the Induction of Antibody Synthesis

Watson

Trenkner

Cohn

1973

The Journal of Experimental Medicine

120

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Evidence is presented that antigen-sensitive cells require two signals for induction. Normally these two signals are delivered to the cell via the recognition of two determinants on the immunogen: the first the receptor on the antigen-sensitive cell, and the second by the cooperating cell system. The special experimental situation described here depends upon the observation that bacterial lipopolysaccharides (LPS) render immunogenic a variety of haptens. When monovalent haptens (TNP-amino acids) are added to spleen cultures, specific antihapten responses are induced in the presence of LPS. After analyzing competing interpretations of this phenomenon, we propose that the antigenic signal is delivered as the consequence of a conformational change in the receptor upon interacting with antigen, and the second signal is delivered directly via the interaction of LPS with the membrane on the antigen-sensitive cell receiving the antigenic signal, or indirectly via the interaction of LPS with the cooperating cell population. These data imply LPS is not itself a mitogen, but merely completes an inductive stimulus to B cells. The experimental results from these and other studies indicate how these two signals may participate in inductive, suppressive, and paralytic stimuli to antigen-sensitive cells.

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“…The following antisera were used: (1) rabbit anti-MOPC 104E mouse myeloma IgM(p, A), (2) rabbit anti-MOPC 2 1 mouse myeloma IgGl (7, K ) , (3) rabbit anti-E. colt 0-galactosidase. The specificity of these antisera have been described [25]. Thus, antiserum (1) detected p-specific, antiserum (2) y-specific determinants.…”

Section: 5mentioning

confidence: 94%