Anti-fungal properties of chitinolytic dune soil bacteria

Boer, Wietse de; Gunnewiek, Paulien J. A. Klein; Lafeber, Petra; Janse, J.; Spit, B. E.; Woldendorp, J. W.

doi:10.1016/s0038-0717(97)00100-4

Cited by 138 publications

(121 citation statements)

References 32 publications

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“…The relevant data reported by the chitinase inhibition against Aspergillus niger (7 mm), Aspergillus flavus (5 mm) (MUHAMAD, 2009). The present result is supported by the previous literature as the chitinolytic bacteria are active against the fungal pathogens (SID AHMED et al, 2003;DE BOER et al,1999). Recently, chitinases have been receiving attention because of their possible application for biological control and gaining importance for their biotechnological applications as it is biodegradable with various applications in the environmental, agricultural, food technology and cosmetics.…”

Section: Antifungal Activitysupporting

confidence: 89%

Production and partial purification of antifungal chitinase from Bacillus cereus VITSD3

Devi¹,

Srinivasan²,

Archana³

et al. 2015

Biosci. J.

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ABSTRACT:The current work was designed to isolate and characterize chitin degrading bacteria. Among the 55 bacterial colonies isolated from 7 different soil samples, 4 isolates were capable of degrading chitinase, among which one strain VITSD3 was found to be potent. Based on the morphological, biochemical and molecular characterization of VITSD3 the isolate was confirmed as Bacillus cereus (Genbank accession number: KC961638), designated as Bacillus cereus VITSD3. The crude enzyme had a total activity of 220 U, precipitated with 44.8 U and 22.5 U for dialysed sample. The hydrolysed product NAG (N-Acetyl Glucosamine) from chitin was analysed by high-pressure liquid chromatography (HPLC).The molecular weight of the chitinase was determined using SDS PAGE and found to be 55 kDa. The partially purified chitinase produced from Bacillus cereus VITSD3 showed antifungal activity against Aspergillus fumigatus (18 mm), Aspergillus niger (6 mm) and Aspergillus flavus (15 mm). Hence the investigation suggests a potential benefit of partially purified chitinase extracted from Bacillus cereus VITSD3 which will serve as an excellent antifungal potential with therapeutic use.

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Section: Antifungal Activitysupporting

confidence: 89%

Production and partial purification of antifungal chitinase from Bacillus cereus VITSD3

Devi¹,

Srinivasan²,

Archana³

et al. 2015

Biosci. J.

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“…It is fair to assume that these two phenomena share underlying mechanisms (Leveau and Preston, 2008). The ectomycorrhizal fungus Laccaria bicolor (Deveau et al, 2007), arbuscular mycorrhizal fungus Glomus mosseae (Pivato et al, 2009) and various soil fungi (de Boer et al, 1998) all showed reduced fungal growth when co-inoculated with Collimonas fungivorans strain Ter331. While the detriment to the fungus was well documented in each case, it was not assessed if and how the bacteria caused it or benefited from it.…”

Section: Introductionmentioning

confidence: 99%

Dual transcriptional profiling of a bacterial/fungal confrontation: Collimonas fungivorans versus Aspergillus niger

et al. 2011

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Interactions between bacteria and fungi cover a wide range of incentives, mechanisms and outcomes. The genus Collimonas consists of soil bacteria that are known for their antifungal activity and ability to grow at the expense of living fungi. In non-contact confrontation assays with the fungus Aspergillus niger, Collimonas fungivorans showed accumulation of biomass concomitant with inhibition of hyphal spread. Through microarray analysis of bacterial and fungal mRNA from the confrontation arena, we gained new insights into the mechanisms underlying the fungistatic effect and mycophagous phenotype of collimonads. Collimonas responded to the fungus by activating genes for the utilization of fungal-derived compounds and for production of a putative antifungal compound. In A. niger, differentially expressed genes included those involved in lipid and cell wall metabolism and cell defense, which correlated well with the hyphal deformations that were observed microscopically. Transcriptional profiles revealed distress in both partners: downregulation of ribosomal proteins and upregulation of mobile genetic elements in the bacteria and expression of endoplasmic reticulum stress and conidia-related genes in the fungus. Both partners experienced nitrogen shortage in each other's presence. Overall, our results indicate that the Collimonas/ Aspergillus interaction is a complex interplay between trophism, antibiosis and competition for nutrients.

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“…All bacterial, fungal and oomycetal strains used in this study (Table 1) are strains from sandy dune soils in The Netherlands (De Boer et al, 1998, de Boer, 2004. Serratia plymuthica PRI-2C strain was isolated from maize rhizosphere, The Netherlands (Garbeva et al, 2004).…”

Section: Bacterial Fungal and Oomycetal Strains And Growth Conditionsmentioning

confidence: 99%

“…The Netherlands (De Boer et al, 1998). Verticillium dahliae JR2 was isolated from tomato, Canada and Rhizoctonia solani AG2.2IIIB was isolated from sugar beet, the Netherlands .…”

Section: Bacterial Fungal and Oomycetal Strains And Growth Conditionsmentioning

confidence: 99%

“…The bacterial strain S. plymuthica PRI-2C was isolated from maize rhizosphere in the Netherlands (Garbeva et al, 2004), pre-cultured from frozen glycerol stocks on 0.1 Tryptic Soy Broth plates (TSB) (Garbeva & de Boer, 2009) and grown for 3 days at 20°C before use. The fungal strain F. culmorum PV was isolated from a sandy dune soil in the Netherlands (De Boer et al, 1998), precultured on 0.5 Potato Dextrose Agar plates (PDA) (Fiddaman & Rossall, 1993) and incubated for 6 days at 20 °C before use.…”

Section: Strains and Growth Conditionsmentioning

confidence: 99%

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Volatile communication between fungi and bacteria

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Anti-fungal properties of chitinolytic dune soil bacteria

Cited by 138 publications

References 32 publications

Production and partial purification of antifungal chitinase from Bacillus cereus VITSD3

Production and partial purification of antifungal chitinase from Bacillus cereus VITSD3

Dual transcriptional profiling of a bacterial/fungal confrontation: Collimonas fungivorans versus Aspergillus niger

Volatile communication between fungi and bacteria

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