Anti‐Fab Antibodies in Lyme Disease

Louie, James S.; Persselin, Jay E.; Shi, G. Y.; Jobe, H; Liebling, Michael R.; Steere, Allen C.; Stevens, Ronald H.

doi:10.1111/j.1749-6632.1988.tb31882.x

Cited by 2 publications

(1 citation statement)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This technique could also assist in our understanding of the pathogenesis of late Lyme disease involving the CNS and the joints. For these later manifestations, it remains unclear whether direct infection, an altered immune response, or a combination of both is responsible (39)(40)(41). Appropriately designed studies employing PCR could help resolve some of these issues.…”

Section: Discussionmentioning

confidence: 99%

The polymerase chain reaction for the detection of borrelia burgdorferi in human body fluids

Liebling

Nishio

Rodriguez

et al. 1993

Arthritis & Rheumatism

View full text Add to dashboard Cite

Objective. To analyze clinical fluids for the presence of Borreliu butgdo#eri DNA using the polymerase chain reaction (PCR).Methods. We utilized a modified, nested PCR to detect the presence of Borreliu DNA in 99 samples of serum, urine, cerebrospinal fluid (CSF), or synovial fluid obtained from 44 patients with various stages of Lyme disease and 47 control subjects. Primer specificity was corroborated by examining 2 DNA data banks, testing against DNA from other organisms, and confirming results with a second set of nested primers.Results. Nested PCR was capable of detecting DNA from fewer than 10 organisms in 1 ml of fluid. The specificity of this technique was 96.4%, with a sensitivity of 76.7%. Although the specificity was uniformly high, the sensitivity was dependent upon the body fluid being tested: CSF loo%, urine loo%, synovial fluid 80%, and serum 59%. The rate of false-positive results was 3.6%.

show abstract

Section: Discussionmentioning

confidence: 99%