Anti-Erythrocyte Membrane Antibodies Detected in Sera of Dogs Naturally Infected with Babsia gibsoni.

Adachi, Kozo; Yoshimoto, Akemi; Hasegawa, Takashi; Shimizu, Takamasa; Goto, Yoshitaka; Makimura, Susumu

doi:10.1292/jvms.54.1081

Cited by 27 publications

(24 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This suggests the possibility that the thrombocytopenia in subclinical B. gibsoni infection is due to autoimmune responses, and the presence of anti-platelet IgG accelerates the platelet destruction in B. gibsoni infected dogs. Anemia is the most common symptom of infection with B. gibsoni [6,9,24], and is thought to be caused by mechanical destruction by the parasite, immunemediated intravascular or extravascular hemolysis, or immune-mediated extravascular hemolysis [2,3,16,17]. Oxidative damage may contribute to the destruction of erythrocytes by increasing their susceptibility to phagocytosis by macrophages [21].…”

Section: Discussionmentioning

confidence: 99%

“…ELISA procedure: The ELISA was performed by a slight modification of the method of Adachi et al [3]. The 96-well ELISA plates (ELISA-Plate, Greiner Bio-one, Germany) were coated with 50 µl of erythrocyte membrane antigens (80 µg protein/ml) or platelet antigens (5 × 10 7 /ml) in 50 mM carbonate buffer (pH 9.6).…”

Section: Identification the Incidence Of B Gibsoni Infection Inmentioning

confidence: 99%

See 1 more Smart Citation

Incidence of Canine Babesia gibsoni Infection and Subclinical Infection among Tosa dogs in Aomori Prefecture, Japan

Matsuu

Kawabe

Koshida

et al. 2004

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

ABSTRACT. To identify the incidence of Babesia gibsoni (B. gibsoni) in Aomori Prefecture, northeastern Japan, dogs with acute B. gibsoni infection were investigated at the Animal Teaching Hospital, Kitasato University, between April 2002 and March 2003. Eighteen dogs with acute B. gibsoni infection were recognized; they were all male dogs of the fighting dog breed Tosa. Their platelet counts were below normal and their packed cell volumes (PCVs) were at various levels. We collected blood samples from 141 Tosa dogs from Aomori Prefecture and used polymerase chain reaction assay to investigate the incidence of subclinical B. gibsoni infection. We also looked into the serological abnormalities associated with thrombocytopenia or anemia in subclinical infection. Forty-one of 87 dogs (47.1%) with histories of dog fighting, and one dog of 54 without a history of dog fighting were positive for B. gibsoni; that is, 42 of 141 dogs (29.8%) showed a positive result. The mean platelet counts of dogs with subclinical infection were significantly lower and levels of anti-platelet IgG were significantly higher than levels for dogs without infection. Anti-erythrocyte membrane IgG levels were significantly higher in dogs with subclinical infections, although mean PCVs were not significantly different. Tosa dogs from Aomori Prefecture, Japan, were highly infected with B. gibsoni subclinically and this pathogen might be successfully transmitted during dog fighting. Dogs with subclinical infections were at risk of chronic thrombocytopenia, which may be due to autoimmune mechanisms. KEY WORDS: anti-erythrocyte membrane IgG, anti-platelet IgG, Babesia gibsoni, Tosa dog.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Identification the Incidence Of B Gibsoni Infection Inmentioning

confidence: 99%

Incidence of Canine Babesia gibsoni Infection and Subclinical Infection among Tosa dogs in Aomori Prefecture, Japan

Matsuu

Kawabe

Koshida

et al. 2004

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

show abstract

“…A proporção superior de resultados falsos positivos no ELISA com CI em relação aos antígenos recombinantes provavelmente ocorreu devido a reações entre resíduos protéicos de eritrócitos, provenientes do processo de produção do antígeno, com anticorpos anti-eritrocíticos. Apesar do desenvolvimento desses anticorpos (anti-eritrocíti-cos) serem um processo patológico já bem documentado para alguns hemoprotozoários como Babesia gibsoni em cães (Adachi et al 1992), Babesia rodhaini e Plasmodium berghei em camundongos (Kanbara et al 1988), em bovinos apenas recentemente foi confirmado com Babesia bigemina (Góes et al 2007). Além disso, Madruga et al (2000) Outro ponto importante nesse trabalho foi a padronização de um ELISA utilizando como antígeno um coquetel de proteínas recombinantes.…”

Section: Resultsunclassified

Comparação entre diversos antígenos para o diagnóstico de Anaplasma marginale por ELISA

et al. 2010

View full text Add to dashboard Cite

Anaplasmose bovina é uma doença com grande importância nas regiões tropicais e subtropicais do mundo por determinar perdas econômicas devido à mortalidade e redução da produtividade. É causada por Anaplasma marginale, uma riquétsia intraeritrocítica obrigatória cujo controle requer, além de uma vacina eficiente, uma acurada identificação de bovinos cronicamente infectados. Apesar de existirem atualmente diversos métodos de diagnóstico dessa riquétsia, os métodos sorológicos, em particular o ensaio de imunoadsorção enzimática-ELISAs, são os mais utilizados devido à sua versatilidade e praticidade. No entanto, devido ao grande número de antígenos disponíveis, atualmente torna-se necessária uma avaliação para definir quais antígenos apresentam um melhor desempenho no diagnóstico da anaplasmose. Soros de bovinos positivos e negativos para A. marginale por PCR, e soros de animais provenientes do Brasil e Costa Rica, foram testados em ELISAs baseados em MSP1a, MSP2 e MSP5 recombinantes, um pool das três proteínas recombinantes, e antígeno de lisado de corpúsculos iniciais da riquétsia (CI). Utilizando soro de bovinos positivos para A. marginale por PCR, uma maior sensibilidade foi observada no ELISA CI. No entanto, uma maior especificidade, com soro de bovinos negativos a PCR, foi observada com os ELISAs recombinantes. O porcentual de bovinos positivos do Brasil e Costa Rica foi maior com ELISA CI. Razões para essas diferenças são discutidas.

show abstract

“…Complement‐ or antibody‐mediated RBC destruction results in hemolysis during IMHA. Immune‐mediated red cell destruction has been associated with the administration of drugs, infectious agents, neoplasia, and other immune‐mediated diseases, such as lupus . In most patients, the inciting cause is not identified and the immune‐mediated anemia is deemed idiopathic.…”

Section: Introductionmentioning

confidence: 99%

Prothrombotic mechanisms and anticoagulant therapy in dogs with immune‐mediated hemolytic anemia

Kidd

Mackman

2013

J Vet Emergen Crit Care

View full text Add to dashboard Cite

show abstract

Anti-Erythrocyte Membrane Antibodies Detected in Sera of Dogs Naturally Infected with Babsia gibsoni.

Cited by 27 publications

References 8 publications

Incidence of Canine Babesia gibsoni Infection and Subclinical Infection among Tosa dogs in Aomori Prefecture, Japan

Incidence of Canine Babesia gibsoni Infection and Subclinical Infection among Tosa dogs in Aomori Prefecture, Japan

Comparação entre diversos antígenos para o diagnóstico de Anaplasma marginale por ELISA

Prothrombotic mechanisms and anticoagulant therapy in dogs with immune‐mediated hemolytic anemia

Contact Info

Product

Resources

About