Anti-enterococcal activities of pentacyclic triterpenes

Wojnicz, Dorota; Tichaczek−Goska, Dorota; Korzekwa, Kamila; Kicia, Marta; Hendrich, Andrzej B.

doi:10.17219/acem/62245

Cited by 15 publications

(21 citation statements)

References 31 publications

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“…However, it should be noted that the MIC values of AA against Gram-positive strains are not always as low as in above-mentioned studies. According to our other previous studies [21], those values against clinical E. faecalis strains were 64-128 µg/mL. Comparable results were obtained by Acebey-Castellon et al [14] against S. aureus ATCC25923 (MIC > 128 µg/mL) and E. faecalis ATCC29212 (MIC = 128 µg/mL).…”

Section: Mics and Mbcs Determinationsupporting

confidence: 60%

“…Under the influence of AA, there was also a decrease in the metabolic activity of bacilli living in single-species biofilms at all stages of their formation. Our previous studies determining the effect of AA on both Gram-negative [ 24 ], and Gram-positive [ 21 ], bacteria, as well as on the formation and eradication of single-species biofilms, showed its antibacterial activity. AA also supported the pharmacological effects of ciprofloxacin when removing mature E. coli biofilm from urinary catheters [ 24 ].…”

Section: Resultsmentioning

confidence: 99%

“…Similar changes were observed in microscopic images of E. coli cultures by Wojnicz et al [ 17 ], who additionally found cells that were thickened, and they had intracellular edema. Wojnicz et al [ 21 ] also conducted research concerning the effects of AA on biofilms formed by clinical E. faecalis strains. The cells of these cocci were larger in terms of diameter, and they formed irregular aggregates instead of chains.…”

Section: Resultsmentioning

confidence: 99%

“…These studies focused primarily on bacteria living in suspension, i.e., planktonic forms. They included the determination of the MIC value (minimum inhibitory concentration) of the acid, its effect on bacterial survival, cell morphology and integrity of the bacterial membrane, and the impact on bacterial virulence factors such as hydrophobicity of the cell’s surface, ability to move, synthesis of curli fimbriae and P-type fimbriae [ 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 ]. Up to date, few studies have investigated the effect of AA on the ability of bacteria to form biofilms, and these studies have mainly concentrated on single-species consortia [ 21 , 22 , 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

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Are Uropathogenic Bacteria Living in Multispecies Biofilm Susceptible to Active Plant Ingredient—Asiatic Acid?

et al. 2021

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Urinary tract infections (UTIs) are a serious health problem in the human population due to their chronic and recurrent nature. Bacteria causing UTIs form multispecies biofilms being resistant to the activity of the conventionally used antibiotics. Therefore, compounds of plant origin are currently being searched for, which could constitute an alternative strategy to antibiotic therapy. Our study aimed to determine the activity of asiatic acid (AA) against biofilms formed by uropathogenic Escherichia coli, Enterobacter cloacae, and Pseudomonas aeruginosa. The influence of AA on the survival, biofilm mass formation by bacteria living in mono-, dual-, and triple-species consortia as well as the metabolic activity and bacterial cell morphology were determined. The spectrophotometric methods were used for biofilm mass synthesis and metabolic activity determination. The survival of bacteria was established using the serial dilution assay. The decrease in survival and a weakening of the ability to create biofilms, both single and multi-species, as well as changes in the morphology of bacterial cells were noticed. As AA works best against young biofilms, the use of AA-containing formulations, especially during the initial stages of infection, seems to be reasonable. However, there is a need for further research concerning AA especially regarding its antibacterial mechanisms of action.

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Section: Mics and Mbcs Determinationsupporting

confidence: 60%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Are Uropathogenic Bacteria Living in Multispecies Biofilm Susceptible to Active Plant Ingredient—Asiatic Acid?

et al. 2021

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“…AA has been shown to suppress growth, cell morphology, virulence factors, and biofilm formation by Enterococcus faecalis strains. It showed potent anti-biofilm activity without affecting hydrophobicity of bacteria and reduced survival as well as virulence (Wojnicz et al, 2017 ). AA isolated from Melastoma malabathricum L. showed antibacterial activity against numerous microbes in agar diffusion method (Wong et al, 2012 ).…”

Section: Asiatic Acid As Antibacterialmentioning

confidence: 99%

Pharmacological Properties, Molecular Mechanisms, and Pharmaceutical Development of Asiatic Acid: A Pentacyclic Triterpenoid of Therapeutic Promise

et al. 2018

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Asiatic acid (AA) is a naturally occurring aglycone of ursane type pentacyclic triterpenoids. It is abundantly present in many edible and medicinal plants including Centella asiatica that is a reputed herb in many traditional medicine formulations for wound healing and neuropsychiatric diseases. AA possesses numerous pharmacological activities such as antioxidant and anti-inflammatory and regulates apoptosis that attributes its therapeutic effects in numerous diseases. AA showed potent antihypertensive, nootropic, neuroprotective, cardioprotective, antimicrobial, and antitumor activities in preclinical studies. In various in vitro and in vivo studies, AA found to affect many enzymes, receptors, growth factors, transcription factors, apoptotic proteins, and cell signaling cascades. This review aims to represent the available reports on therapeutic potential and the underlying pharmacological and molecular mechanisms of AA. The review also also discusses the challenges and prospects on the pharmaceutical development of AA such as pharmacokinetics, physicochemical properties, analysis and structural modifications, and drug delivery. AA showed favorable pharmacokinetics and found bioavailable following oral or interaperitoneal administration. The studies demonstrate the polypharmacological properties, therapeutic potential and molecular mechanisms of AA in numerous diseases. Taken together the evidences from available studies, AA appears one of the important multitargeted polypharmacological agents of natural origin for further pharmaceutical development and clinical application. Provided the favorable pharmacokinetics, safety, and efficacy, AA can be a promising agent or adjuvant along with currently used modern medicines with a pharmacological basis of its use in therapeutics.

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Secondary Metabolite Analysis of Phomopsis sp. LGT‐5 Based on the Dual Guidance of Whole‐Genome Sequencing and HPLC‐Q‐ToF‐MS/MS

Zhu

et al. 2023

Chemistry & Biodiversity

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Microorganisms produce a wealth of structurally diverse specialized metabolites with a remarkable range of biological activities. The Phomopsis sp. LGT‐5 was obtained through tissue block and repeatedly crossed methods from Tripterygium wilfordii Hook. F. The antibacterial experiments of LGT‐5 showed that it has high inhibitory activity against Staphylococcus aureus and Pseudomonas aeruginosa, and moderate inhibitory activity against Candida albicans. To research the generation of the antibacterial phenomenon of LGT‐5 and provide support for further research and application, the whole genome sequencing (WGS) of LGT‐5 was obtained by single‐molecule real‐time DNA sequencing platform Pacific Biosciences (PacBio) sequencing and Illumina paired‐end sequencing. The final assembled LGT‐5 genome is 54.79 Mb with a contig N50 of 290.07 kb; in addition, its secondary metabolites were detected through HPLC‐Q‐ToF‐MS/MS. By comparing its MS/MS data, the secondary metabolites were analyzed based on visual network maps obtained on the Global Natural Products Social Molecular Networking (GNPS). The analysis results showed that the secondary metabolites of LGT‐5 were triterpenes and various cyclic dipeptides.

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Anti-enterococcal activities of pentacyclic triterpenes

Abstract: Based on the results obtained, we can assume that the triterpenes investigated should be considered natural components of a human diet rather than as antibacterial agents used on their own.

Cited by 15 publications

References 31 publications

Are Uropathogenic Bacteria Living in Multispecies Biofilm Susceptible to Active Plant Ingredient—Asiatic Acid?

Are Uropathogenic Bacteria Living in Multispecies Biofilm Susceptible to Active Plant Ingredient—Asiatic Acid?

Pharmacological Properties, Molecular Mechanisms, and Pharmaceutical Development of Asiatic Acid: A Pentacyclic Triterpenoid of Therapeutic Promise

Secondary Metabolite Analysis of Phomopsis sp. LGT‐5 Based on the Dual Guidance of Whole‐Genome Sequencing and HPLC‐Q‐ToF‐MS/MS

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