Anti-EBV Activity of Hemocyanin Isolated from Helix Lucorum

Zagorodnya, Svіtlana; Dolashka, Pavlina; Баранова, Г. В.; Golovan, Anna; Nesterova, N. V.

doi:10.1016/j.antiviral.2011.03.134

Cited by 8 publications

(5 citation statements)

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“…Haemocyanins and haemocyanin-derived peptides from marine and terrestrial gastropods have potent anti-herpesvirus activity Zagorodnya et al, 2011;Zanjani et al, 2014). The primary function of haemocyanins is the transport of molecular oxygen to respiring tissues (Coates & Nairn, 2014;Zanjani et al, 2014), but their contribution to innate immunity is often overlooked (Coates & Nairn, 2014).…”

Section: Antiviral Compoundsmentioning

confidence: 99%

Antiviral immunity in marine molluscs

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et al. 2015

Journal of General Virology

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Marine molluscs, like all living organisms, are constantly exposed to viruses and have evolved efficient antiviral defences. We review here recent developments in molluscan antiviral immunity against viruses belonging to the order Herpesvirales. Emerging results suggest an interferon-like response and autophagy are involved in the antiviral defence of bivalves to viral infection. Multifunctional plasma proteins from gastropods and bivalves have been identified to have broadspectrum antiviral activity against mammalian viruses. The antiviral defences present in molluscs can be enhanced by genetic selection, as shown by the presence of oyster strains specifically resistant to ostreid herpesvirus type 1. Whether varying amounts or different isoforms of these antiviral plasma proteins contributes to genetic resistance is worthy of further research. Other evolutionarily conserved antiviral mechanisms, such as RNA interference and apoptosis, still need further characterization.

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Section: Antiviral Compoundsmentioning

confidence: 99%

Antiviral immunity in marine molluscs

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Raftos

Speck

et al. 2015

Journal of General Virology

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“…15,16 Moreover, also the antiviral activity of molluscan hemocyanins (RvH, β-HlH, KLH, and Haliotis tuberculata hemocyanin (HtH)), which is also based on the presence of carbohydrate structures, has recently drawn considerable attention. [17][18][19][20][21][22][23][24][25] The carbohydrate content of molluscan hemocyanins (2-9%, w/w) is higher than that of most arthropod hemocyanins. 26,27 Moreover, hemocyanins of Helix pomatia (HpH) and Lymnaea stagnalis contain monosaccharides not usually found in animal glycoproteins (H. pomatia: D-xyl and 3-O-methyl-D-galactose (3MeGal) 28,29 ; L. stagnalis: D-Xyl, 3-O-methyl-D-mannose (3MeMan), and 3MeGal) 30 .…”

Section: Introductionmentioning

confidence: 99%

N-glycan structures of β-HlH subunit of Helix lucorum hemocyanin

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Dolashka

Beeumen

et al. 2017

Carbohydrate Research

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The carbohydrate structures of molluscan hemocyanins have recently received particular interest due to their specific monosaccharide composition, as well as their immunostimulatory properties and application in clinical studies. For the first time, we investigated N-glycans of the structural subunit β-HlH of hemocyanin isolated from Helix lucorum. In total, 32 different glycans were enzymatically liberated and characterized by tandem mass spectrometry using a Q-Trap mass spectrometer. Our study revealed a highly heterogeneous mixture of glycans with composition HexHexNAcMeHexPentFuc. The oligosaccharide chains are mostly modified at the inner core by β1-2-linked xylose to β-mannose, by α1-6-fucosylation of the innermost GlcNAc residue (the Asn-bound GlcNAc), and by methylation. The glycans of β-HlH mainly contain a terminal MeHex residue; in some cases even two, three or four of these residues occur. Several carbohydrate chains in β-HlH are core-fucosylated without Xyl and also possess a high degree of methylation. This study shows the presence of mono- and bi-antennary N-glycans as well as hybrid type structures with or without core-fucosylation.

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“…Further research revealed that HMC may be a novel and important non-specific innate immune defense molecule (2,3). It has been reported that HMC could be functionally converted into phenoloxidase (4) antimicrobial peptides (5)(6)(7)(8), antiviral (9)(10)(11)(12), antitumor agent (13)(14)(15)(16)(17), antifungal (18), agglutinin (19,20), hemolysin (21), and regulatory proteins (22,23). Moreover, our previous findings indicated that HMC from shrimp Litopenaeus vannamei reacted with human Ig as an antigen (5,19,24) and acted as an immune-enhancing protein (25,26).…”

Section: Introductionmentioning

confidence: 99%

Protein Diversity and Immune Specificity of Hemocyanin From Shrimp Litopenaeus vannamei

et al. 2021

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Hemocyanin is an important non-specific innate immune defense molecule with phenoloxidase, antiviral, antibacterial, hemolytic, and antitumor activities. To better understand the mechanism of functional diversity, proteomics approach was applied to characterize hemocyanin (HMC) expression profiles from Litopenaeus vannamei. At first, hemocyanin was purified by Sephadex G-100 and DEAE-cellulose (DE-52) columns from shrimp serum, and 34 protein spots were identified as HMC on the 2-DE gels. Furthermore, we found that 9 HMC spots about 75 or 77 kDa were regulated by Streptococcus agalactiae and Vibrio parahaemolyticus infection at 6, 12, and 24 h. In addition, 6 different pathogen-binding HMC fractions, viz., HMC-Mix, HMC-Vp, HMC-Va, HMC-Vf, HMC-Ec, and HMC-Sa, showed different agglutinative and antibacterial activities. Moreover, lectin-blotting analysis showed significant differences in glycosylation level among HMC isomers and bacteria-binding HMC fractions. Particularly, the agglutinative activities of the HMC fractions were almost completely abolished when HMC was deglycosylated by O-glycosidase, which suggest that O-linked sugar chains of HMC played important roles in the innate immune recognition. Our findings demonstrated for the first time that L. vannamei HMC had molecular diversity in protein level, which is closely associated with its ability to recognize diverse pathogens, whereas glycan modification probably contributed to HMC’s diversity and multiple immune activities.

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Anti-EBV Activity of Hemocyanin Isolated from Helix Lucorum

Cited by 8 publications

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Antiviral immunity in marine molluscs

Antiviral immunity in marine molluscs

N-glycan structures of β-HlH subunit of Helix lucorum hemocyanin

Protein Diversity and Immune Specificity of Hemocyanin From Shrimp Litopenaeus vannamei

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