Anti-dsDNA antibodies in systemic lupus erythematosus: A combination of two quantitative methods and the ANA pattern is the most efficient strategy of detection

González, Delia Almeida; Varela, Alfredo Roces; Rodríguez, Itahisa Marcelino; Vera, Alexander González; Sánchez, Mónica Delgado; Esquivel, Antonio Aznar; Rodríguez, C. Casañas; León, Antonio Cabrera de

doi:10.1016/j.jim.2015.09.003

Cited by 11 publications

(6 citation statements)

References 22 publications

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“…Positivity of ANA with indirect immunoflourence testing approaches above 95% using HEP-2 cell line 20. We found ANA positivity in 90.2% of patients and it is almost similar to previous studies.…”

Section: Discussionsupporting

confidence: 90%

Pattern of initial clinical manifestations of systemic lupus erythematosus in a tertiary care hospital

Batool¹,

Ahmad²,

Saeed³

et al. 2016

Pak J Med Sci

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Objective:To determine the pattern of initial clinical manifestations of Systemic Lupus Erythematosus (SLE) and to compare these features with those recorded elsewhere in PakistanMethods:This cross-sectional, descriptive study was performed in the Department of Rheumatology, Fatima Memorial Hospital, Lahore, Pakistan, from November 2015 to January 2016. Sixty one patients of SLE diagnosed as per ACR (American College of Rheumatology) 1982 revised criteria, were enrolled. The patients were evaluated for the initial clinical manifestations of SLE. The information was collected on a specially designed proforma and analyzed by using SPSS version 17.Results:Out of 61 patients, 49 (80.3%) were females and 12 (19.7%) males, showing a female to male ratio of 4:1. The mean age of patients was 26.2 ± 7.9 years. Fatigue was the most common presenting feature in 56 (91.8%) patients, followed by joint pains in 55 (90.2%) and fever in 54 (88.5%). Renal involvement was found in 46 (75.4%). Comparison of these presenting features was made with other studies carried out in Northern Pakistan (Islamabad) and in central Punjab (Pakistan). There were statistically significant differences in fever, fatigue and arthritis between our patients and the other two above mentioned study groups. However, comparison of renal manifestations showed significant difference only with Islamabad study, and not with previous study from central Punjab.Conclusion:In this study, majority of patients presented with combination of fatigue, fever, rash and arthritis. Almost three-fourth of patients had renal manifestations at initial presentation. Therefore, it is important for clinicians to have high index of suspicion for SLE, when patients present with above symptoms.

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Section: Discussionsupporting

confidence: 90%

Pattern of initial clinical manifestations of systemic lupus erythematosus in a tertiary care hospital

Batool¹,

Ahmad²,

Saeed³

et al. 2016

Pak J Med Sci

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“…The sensitivity of ANAs is very high as a screening tool which is about up to more than 95%. 18 But we had found in our study that all SLE patients were positive for ANAs. Anti-double-stranded DNA antibodies are more specific than ANAs, but are carrying lower sensitivity (60%).…”

Section: Discussionmentioning

confidence: 49%

A Prospective Study to evaluate Pregnancy Outcomes in Patients with Systemic Lupus Erythematosus

Ghosh¹,

Tiwari²,

Biswas³

et al. 2018

Journal of South Asian Federation of Obstetrics and Gynaecology

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Aim: Study of systemic lupus erythematosus (SLE) with pregnancy to manage them in a multidisciplinary approach for better pregnancy outcomes. Materials and methods:This study was a prospective cohort study. A total of 100 pregnant women with diagnosed SLE were included in the study and another 100 age-matched normal pregnant women without any obvious complications were recruited as controls.Results: Maternal organ involvement-five patients of renal involvement and seven patients of cardiac and pulmonary involvement in SLE group-was found. Antinuclear antibody (ANA) was positive in all cases and 87 were positive for antidouble-stranded deoxyribonucleic acid (anti-dsDNA) antibody. The disease flare was found only in a single case of planned pregnancy and total nine cases of unplanned pregnancy. Most of the women, 41 (53.25%), in the SLE group were delivered by cesarean section, but only 24 (25%) in the control group underwent cesarean section. Conclusion:The SLE with pregnancy is a high-risk condition where prepregnancy disease-free interval is the most important criteria to minimize complications. Hydroxychloroquine can be used safely throughout the pregnancy. Multidisciplinary approach plays a crucial part in management.Clinical significance: Management of lupus flares and preeclampsia during SLE pregnancy is being treated with difficulties with overlapping clinical features. Presence of antiphospholipid antibodies (APLA) is a major unresolved issue. Adverse events to maternal and fetal well-being with use of appropriate medications are required for optimum outcomes.

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“…It must take into consideration the clinical picture and symptoms of the disease and anti-dsDNA antibodies. On the bases of these facts, the ANA should be companied by anti-dsDNA antibodies in SLE-suspected individuals [24]. Both ANA and dsDNA were raised in this study of SLE patients and both are significantly associated with SLE and had 100% sensitivity and 100% specificity.…”

Section: P-valuementioning

confidence: 59%

Role of some Immunological and non-Immunological Parameters in Prognosis and diagnosis of Systemic lupus erythematosus in Iraqi women patients

Jassim,

Ali,

Auda

et al. 2023

Al-Mustansiriyah Journal of Science

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Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by autoantibody's presence in the circulation and involvement of many systems. The current study aims to study the role of some immunological and non-immunological parameters in SLE development and to illustrate the correlations between these parameters. The current study included 50 blood samples collected from SLE patients and apparently healthy control. The methods of work included Complete Blood Count (CBC) analysis to assess blood components, you mean to analyse Hb, WBC, ESR, ELISA technique to measure serum levels of TLR-7, IL-17, and TNF-γ, antinuclear antibody test (ANA) and double-strand DNA antibody (anti ds DNA). The study results showed a significant increase in serum levels of each WBC (p< 0.005), ESR (p< 0.001), IL17(p< 0.004), TLR7(p< 0.005), IFN-γ (p< 0.001), ANA (p< 0.001), and anti dsDNA (p< 0.001) in SLE patients compared to apparently healthy control but the Hb is decreasing level was low in the SLE patients compared with control. In conclusion, the low hemoglobin, WBC, and ESR are non-specific parameters associated with SLE pathogenesis. ANA, Anti-ds-DNA as excellent biomarkers for the diagnosis of SLE and serum level of TLR-7, TNF-γ, and IL-17 were evaluated in SLE patients compared to healthy people and they may be suggested as a prognostic tool in SLE patients.

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Anti-dsDNA antibodies in systemic lupus erythematosus: A combination of two quantitative methods and the ANA pattern is the most efficient strategy of detection

Cited by 11 publications

References 22 publications

Pattern of initial clinical manifestations of systemic lupus erythematosus in a tertiary care hospital

Pattern of initial clinical manifestations of systemic lupus erythematosus in a tertiary care hospital

A Prospective Study to evaluate Pregnancy Outcomes in Patients with Systemic Lupus Erythematosus

Role of some Immunological and non-Immunological Parameters in Prognosis and diagnosis of Systemic lupus erythematosus in Iraqi women patients

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