Background: The use of dietary supplements without adequate knowledge regarding their constituents places professional athletes at great risk due to possibility of being positive in dope testing. The aim of this study was to identify the dietary supplements usage patterns among elite athletes in various sport types and investigate their knowledge and attitudes regarding that.Methods: A cross-sectional study was conducted in which a self -administered anonymous questionnaire was used to collect data from 387 elite athletes participating in individual (athletics, karate, weightlifting, wrestling and shooting), team (cricket, volleyball, netball, rugby, football, kabaddi and hockey) and both (wushu and badminton) sports. The data were analysed using nonparametric tests; Pearson’s chi square test with the level of significant of p < 0.05.Results: Out of 387 athletes, 354 (91.5%) used dietary supplements, multivitamin (57%), electrolyte (49.7%), protein (43.5%), calcium (19.7%) and creatine (17.4%) as the most prevalent types. Gender, social status, educational level, and professionalism had no impact with the supplement use whereas the type of sport played showed a significant impact on supplement intake (p < 0.05), with athletes in netball, rugby, wrestling, badminton and shooting showed 100% usage. Vitamin, protein and creatine usage were high among rugby, netball and weightlifting players (p < 0.05). The majority of athletes consume supplements to improve their performance (41.8%) and energy (54. 5%). Further,60.5% of the athletes claimed on self-prescription without medical advice. Two perceptions of, supplements are essential for maintain fitness and win awards were significantly high in males (p <0.05). Athletes having participated in educational workshops and competed internationally showed significant concern about the contamination risk associated with supplements (p < 0.05). Rugby, weightlifting and netball players showed significant multiple supplement usage among others (p <0.05).Conclusion: This study confirms that dietary supplements are readily used by Sri Lankan elite athletes, while multivitamins, protein and energy drink products are preferred. Additionally, the majority of athletes engaged in supplement use with the aim of performance enhancement and increase energy. Nature of sport played was a determinant factor of net supplement usage while higher multiple supplement usage appeared among intensive sports.