Anti-diabetic property of Tinospora cordifolia and its active compound is mediated through the expression of Glut-4 in L6 myotubes

Sangeetha, M.; Priya, C.D.Mohana; Vasanthi, Hannah R.

doi:10.1016/j.phymed.2012.11.006

Cited by 70 publications

(34 citation statements)

References 14 publications

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“…In vitro activation of PPARα by T. cordifolia has been reported earlier. 9 This study confirms the in vitro result and validates in vivo activity of TCJ as PPARα modulator.…”

Section: 13supporting

confidence: 85%

“…15 Compounds isolated from the plants were also reported to increase glucose uptake activity by increasing GLUT-4 expression. 9 Moreover, T. cordifolia is well known for in vivo immunomodulatory effects, 16 however, immunomodulatory compounds from the plant, yet to be identified. A number of studies have been conducted to explore the medicinal properties of crude extracts and isolated pure compounds.…”

Section: 13mentioning

confidence: 99%

“…9 A number of in vivo studies have already been conducted to validate the in vitro efficacy of the plant extracts. 10 However, it is well known fact that in vitro mechanism of drugs cannot be correlated with in vivo mechanism due to the complex phenomenon which starts from gut absorption of compounds to their catabolism.…”

mentioning

confidence: 99%

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Administration of Fresh Juice of Tinospora cordifolia Decreases Levels of Urinary Markers of Peroxisome Proliferator-Activated Receptors in Hyperlipidemic Patients

Shirolkar¹,

Dabur²

2016

IJPER

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Objective: Tinospora cordifolia (Willd.) Miers juice (TCJ) is known for its anti-hyperlipidemic properties. This study was aimed to validate the efficacy of TCJ to activate PPARα in vivo in hyperlipidemic (HPL) patients. Materials and Methods: HPL (n=20) and healthy (n=22) volunteers were enrolled in the study. HPL patients were treated with TCJ for 14 days. The fasting urine and blood samples were collected on 0 and 14 th day for analysis. The blood samples were used for biochemical analysis. Urine samples were subjected to LC-ESI-QTOFMS for the analysis of PPAR-α markers. Results: Treatment with TCJ depleted cholesterol (TC-24%), triglycerides (TG-28%) and low density lipoprotein (LDL-14%) levels and increased high density lipoprotein (HDL) (p<0.05) levels in HPL. The QTOFMS data revealed increased urinary excretions of pantothenic acid (5.99 fold), hippuric acid (<15.0 fold), acylcarnitines (<2.0 folds) and branched chain amino acids (BCAA) in HPL patients which significantly depleted (p<0.01) after TCJ treatment. Two new acylcarnitines, L-hexanoylcarnitine and 4-hydroxyisovaleric acid observed in HPL patients were depleted (>2.0 fold) after treatment with TCJ. The other biomarkers of HPL, ursodeoxycholic acid and chenodeoxycholic acid were also depleted (>4.0 fold) after TCJ treatment. Conclusion: The present data clearly depicted the regulatory effects of TCJ on the lipid metabolism. It emphasize that TCJ activates PPAR-α in vivo that decreased the urinary levels of PPARα biomarkers.

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“…In vitro activation of PPARα by T. cordifolia has been reported earlier. 9 This study confirms the in vitro result and validates in vivo activity of TCJ as PPARα modulator.…”

Section: 13supporting

confidence: 85%

Section: 13mentioning

confidence: 99%

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Administration of Fresh Juice of Tinospora cordifolia Decreases Levels of Urinary Markers of Peroxisome Proliferator-Activated Receptors in Hyperlipidemic Patients

Shirolkar¹,

Dabur²

2016

IJPER

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“…An extensive literature survey revealed 11 plants having proven antidiabetic properties that are largely distributed across the geographical niche of NE India. The plants include: Ananas comosus (pineapple), Carica papaya (papaya), Catharanthus roseus (noyontara), Citrus aurantium (bitter orange), Cynodon dactylon (bermuda grass), Dioscorea alata (ratalu), Jatropha curcas (barbados nut), Mangifera indica (mango), Morus indica (mulberry), Nelumbo nucifera (lotus), and Tinospora cordifolia (guduchi) (Aderibigbe et al, 1999;Xie et al, 2005;Huralikuppi et al, 2006;Jarald et al, 2008;Sharma et al, 2008;Mishra et al, 2010;Rasineni et al, 2010;Kumar et al, 2010;Maithili et al, 2011;Juarez-Rojop et al, 2012;Sangeetha et al, 2013). Although much progress have been made in recent years in many medicinal plants, the study of genetic potential and improvement through molecular breeding has not been attempted to date for the above mentioned plants.…”

Section: Introductionmentioning

confidence: 99%

Rediscovering Medicinal Plants' Potential with OMICS: Microsatellite Survey in Expressed Sequence Tags of Eleven Traditional Plants with Potent Antidiabetic Properties

Sahu

Sen

Choudhury

et al. 2014

OMICS: A Journal of Integrative Biology

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Herbal medicines and traditionally used medicinal plants present an untapped potential for novel molecular target discovery using systems science and OMICS biotechnology driven strategies. Since up to 40% of the world's poor people have no access to government health services, traditional and folk medicines are often the only therapeutics available to them. In this vein, North East (NE) India is recognized for its rich bioresources. As part of the Indo-Burma hotspot, it is regarded as an epicenter of biodiversity for several plants having myriad traditional uses, including medicinal use. However, the improvement of these valuable bioresources through molecular breeding strategies, for example, using genic microsatellites or Simple Sequence Repeats (SSRs) or Expressed Sequence Tags (ESTs)-derived SSRs has not been fully utilized in large scale to date. In this study, we identified a total of 47,700 microsatellites from 109,609 ESTs of 11 medicinal plants (pineapple, papaya, noyontara, bitter orange, bermuda brass, ratalu, barbados nut, mango, mulberry, lotus, and guduchi) having proven antidiabetic properties. A total of 58,159 primer pairs were designed for the non-redundant 8060 SSRpositive ESTs and putative functions were assigned to 4483 unique contigs. Among the identified microsatellites, excluding mononucleotide repeats, di-/trinucleotides are predominant, among which repeat motifs of AG/CT and AAG/CTT were most abundant. Similarity search of SSR containing ESTs and antidiabetic gene sequences revealed 11 microsatellites linked to antidiabetic genes in five plants. GO term enrichment analysis revealed a total of 80 enriched GO terms widely distributed in 53 biological processes, 17 molecular functions, and 10 cellular components associated with the 11 markers. The present study therefore provides concrete insights into the frequency and distribution of SSRs in important medicinal resources. The microsatellite markers reported here markedly add to the genetic stock for cross transferability in these plants and the literature on biomarkers and novel drug discovery for common chronic diseases such as diabetes.

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“…The main chemical constituents of the plant include berberine, tinosporin, tinosporal, tinosporaside, tinosporic acid, tinocordiofolioside, columbin, etc. ; all of which add to its medicinal property [8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Phloroglucinol Plays Role in Shoot Bud Induction and In Vitro Tuberization in Tinospora Cordifolia- A Medicinal Plant with Multi- Therapeutic Application

Kumar¹

2015

Adv Tech Biol Med

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This paper shows the potential of phloroglucinol for In Vitro regeneration and tuberization of microshoots of Tinospora cordifolia. Within a week of nodal explants culture, axillary bud in nodal segments sprouted in all the media investigated including basal MS media without any growth regulators. Maximum response (52.2 per cent) evinced by MS media supplemented with 6.98 µM Kin. The same media produced maximum response for multiple shoot formation out of total shoot bud sprouted fostering average 3 shoots with 3.9 cm shoot length and 4.2 number of leaves per shoot. Effect of PG also investigated for mass multiplication and In Vitro rooting. Basal MS + 6.98 µM Kin + 79.4 µM PG increased shoot bud induction from 52.2 per cent to 84.8 per cent and multiple shoot production per cent from 12.9 to as high as 60.3 percent and also supported maximum number of shoot per explants (7.5). The same treatment promoted highest axillary shoot proliferation in terms of shoot length (3.9 cm) and number of leaves per shoot (4.3). Phloglucinol induced In Vitro tuberization in microshoots. Maximum rooting response observed in ½ MS medium supplemented with 7.4 µM IBA and 793.7 µM PG with 81.1 culture responding taking just 8.7 days to sprout and producing maximum number of roots (3.2) with an average length (3.1 cm). Rooted explants successfully hardened in Soil: Vermicompost: Vermiculite (1:1:1) with 100% success.

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Anti-diabetic property of Tinospora cordifolia and its active compound is mediated through the expression of Glut-4 in L6 myotubes

Cited by 70 publications

References 14 publications

Administration of Fresh Juice of Tinospora cordifolia Decreases Levels of Urinary Markers of Peroxisome Proliferator-Activated Receptors in Hyperlipidemic Patients

Administration of Fresh Juice of Tinospora cordifolia Decreases Levels of Urinary Markers of Peroxisome Proliferator-Activated Receptors in Hyperlipidemic Patients

Rediscovering Medicinal Plants' Potential with OMICS: Microsatellite Survey in Expressed Sequence Tags of Eleven Traditional Plants with Potent Antidiabetic Properties

Phloroglucinol Plays Role in Shoot Bud Induction and In Vitro Tuberization in Tinospora Cordifolia- A Medicinal Plant with Multi- Therapeutic Application

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