Anti-diabetic effects of the silkworm (Bombyx mori.) extracts in the db/db mice

Ryu, Kyeong‐Sik; Hs, Lee; Ky, Kim; Kim, Do Young; Kang, PD; Chun, SN; Lim, SH; Ml, Lee

doi:10.1055/s-0032-1321145

Cited by 9 publications

(1 citation statement)

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“…The CTB-APSL fusion protein was enclosed by lipidosome in the pupae, and these protease inhibitors and lipidosome may have a positive effect on helping the CTB-APSL fusion protein avoid the gastrointestinal digestive enzymes. In addition, the extracts from silkworm also have anti-diabetic effects [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

Anti-Diabetic Effects of CTB-APSL Fusion Protein in Type 2 Diabetic Mice

et al. 2014

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To determine whether cholera toxin B subunit and active peptide from shark liver (CTB-APSL) fusion protein plays a role in treatment of type 2 diabetic mice, the CTB-APSL gene was cloned and expressed in silkworm (Bombyx mori) baculovirus expression vector system (BEVS), then the fusion protein was orally administrated at a dose of 100 mg/kg for five weeks in diabetic mice. The results demonstrated that the oral administration of CTB-APSL fusion protein can effectively reduce the levels of both fasting blood glucose (FBG) and glycosylated hemoglobin (GHb), promote insulin secretion and improve insulin resistance, significantly improve lipid metabolism, reduce triglycerides (TG), total cholesterol (TC) and low density lipoprotein (LDL) levels and increase high density lipoprotein (HDL) levels, as well as effectively improve the inflammatory response of type 2 diabetic mice through the reduction of the levels of inflammatory cytokines tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). Histopathology shows that the fusion protein can significantly repair damaged pancreatic tissue in type 2 diabetic mice, significantly improve hepatic steatosis and hepatic cell cloudy swelling, reduce the content of lipid droplets in type 2 diabetic mice, effectively inhibit renal interstitial inflammatory cells invasion and improve renal tubular epithelial cell nucleus pyknosis, thus providing an experimental basis for the development of a new type of oral therapy for type 2 diabetes.

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Section: Discussionmentioning

confidence: 99%