Anti-diabetic effects of lactic acid bacteria in normal and type 2 diabetic mice

Abstract

The antidiabetic effects of lactic acid bacteria were investigated using mice. In Experiment 1, normal ICR mice were loaded with sucrose or starch with or without viable Lactobacillus rhamnosus GG cells. GG significantly inhibited postprandial blood glucose levels when administered with sucrose or starch. In Experiment 2, KK-Ay mice, a model of genetic type 2 diabetes, were given a basal diet containing viable GG cells or viable Lactobacillus delbrueckii subsp. bulgaricus cells for 6 weeks. Viable GG cells significantly inhibited fasting blood glucose, postprandial blood glucose in a glucose tolerance test and HbA1c. Such effects were not shown by viable L. bulgaricus cells. In Experiment 3, the KK-Ay mice were given a basal diet containing viable GG cells or heat-treated GG cells for 3 weeks. The viable GG cells significantly suppressed fasting blood glucose and impaired glucose tolerance, but the heat-treated GG showed no effects. These results demonstrated that GG decreased the postprandial blood glucose in ICR mice, and that the antidiabetic activity of lactic acid bacteria on the KK-Ay mice differed depending on the bacterial strain and whether the bacterium is viable when it arrives in the intestine. In the present study, we conclude that the antidiabetic activity may result from continuous inhibition of the postprandial blood glucose through suppression of glucose absorption from the intestine. These findings indicate that specific strains of lactic acid bacterium can be expected to be beneficial for the management of type 2 diabetes.

Probiotics as beneficial agents in the management of diabetes mellitus: a systematic review

“…They, eventually, found that only viable L . rhamnosus GG suppressed the elevated FPG in both groups ( p < 0.05) . Concerning the effects of different bacterial strain, El‐Khamisy et al .…”

“…Ultimately, 33 articles were included in the final analysis, consisting of five human and 28 animal reports . Information on human and animal studies in relation to methodological quality, characteristics of participants, interventions and outcome measures are summarized in Tables and .…”

“…. The significant reduction in FPG levels was seen in probiotic groups of these ten studies, comparing the following experimental groups: diabetic and non‐diabetic groups that received placebos, groups that received high fat or high fructose diets administered for inducing diabetes in rats, groups that received fruit and vegetable fermented diet and groups that received skimmed milk . Although Lactobacillus or Lactococcus strains were in common in the interventions of these studies, various sub‐strains were used, such as L .…”

“…Honda et al . also had both 3 and 6 weeks durations of intervention in their study ; they believed that the effects of probiotic differ depending on the bacterial strain and whether the bacterium is viable when it arrives in the intestine. Thus, they assessed the differences between the effects of viable L .…”

Partially Purified Exopolysaccharide from Lactobacillus plantarum YML009 with Total Phenolic Content, Antioxidant and Free Radical Scavenging Efficacy

“…However, in future, the partially purified EPS from L. plantarum YML009 could be of use in various clinical therapies for the treatment of various chronic diseases such as cancer and diabetes as also evident by others. 35,36 Research can also be elaborated on human trials since most of the LAB based compounds are known as GRAS. Hence, this partially purified may also prove its usefulness in drug discovery sector.…”