Anti-Diabetic Effects of Ethanol Extract from Sanghuangporous vaninii in High-Fat/Sucrose Diet and Streptozotocin-Induced Diabetic Mice by Modulating Gut Microbiota

Abstract

Type 2 diabetes mellitus (T2DM) may lead to abnormally elevated blood glucose, lipid metabolism disorder, and low-grade inflammation. Besides, the development of T2DM is always accompanied by gut microbiota dysbiosis and metabolic dysfunction. In this study, the T2DM mice model was established by feeding a high-fat/sucrose diet combined with injecting a low dose of streptozotocin. Additionally, the effects of oral administration of ethanol extract from Sanghuangporous vaninii (SVE) on T2DM and its complications (including hypoglycemia, hyperlipidemia, inflammation, and gut microbiota dysbiosis) were investigated. The results showed SVE could improve body weight, glycolipid metabolism, and inflammation-related parameters. Besides, SVE intervention effectively ameliorated the diabetes-induced pancreas and jejunum injury. Furthermore, SVE intervention significantly increased the relative abundances of Akkermansia, Dubosiella, Bacteroides, and Parabacteroides, and decreased the levels of Lactobacillus, Flavonifractor, Odoribacter, and Desulfovibrio compared to the model group (LDA > 3.0, p < 0.05). Metabolic function prediction of the intestinal microbiota by PICRUSt revealed that glycerolipid metabolism, insulin signaling pathway, PI3K-Akt signaling pathway, and fatty acid degradation were enriched in the diabetic mice treated with SVE. Moreover, the integrative analysis indicated that the key intestinal microbial phylotypes in response to SVE intervention were strongly correlated with glucose and lipid metabolism-associated biochemical parameters. These findings demonstrated that SVE has the potential to alleviate T2DM and its complications by modulating the gut microbiota imbalance.

Sanghuangporus vaninii mixture ameliorated type 2 diabetes mellitus and altered intestinal microbiota in mice

“…26 On the other hand, some recent studies showed that different extracts of “Sanghuang” could modulate intestinal microbiota imbalance to alleviate diabetes, including water extract (polysaccharide) of Phellinus linteus 26 and ethanol extract of Sanghuangporus vaninii . 27 Hence, this study established a T2DM mouse model to evaluate the effect of a mixture of different “Sanghuang” extracts on the biochemical indicators in T2DM mice, and the regulatory effect on the intestinal microbiota in T2DM mice was also analyzed through 16S rRNA sequencing. It also revealed the relationship between biochemical indicators and the intestinal microbiota, providing theoretical guidance for developing “Sanghuang” as a functional food to prevent T2DM.…”

Sanghuangporus vaninii fruit body polysaccharide alleviates hyperglycemia and hyperlipidemia via modulating intestinal microflora in type 2 diabetic mice

“…Recently, intestinal microflora has received great attention, and its structure and stability are important for the maintenance of intestinal balance ( 40 ). Studies have shown that altering specific microorganisms in the intestine can affect the development of diabetes ( 41 , 42 ). In this study, 16S rRNA based sequencing analysis was conducted to investigate the effects of SVP on the structure of intestinal microflora.…”

Blood Sugar, Haemoglobin and Malondialdehyde Levels in Diabetic White Rats Fed a Diet of Corn Flour Cookies

“…As changes in food and nutritional status have a strong influence on body weight, this is one of the anthropometric measurements that is frequently used for assessing nutritional status [ 28 ]. Insulin deficiency in diabetics is known to disrupt protein and fat metabolism, resulting in weight loss ([ 59 ].…”