Anti-diabetic effects of Caulerpa lentillifera: stimulation of insulin secretion in pancreatic β-cells and enhancement of glucose uptake in adipocytes

Sharma, Bhesh Raj; Rhyu, Dong Young

doi:10.12980/apjtb.4.2014apjtb-2014-0091

Cited by 71 publications

(46 citation statements)

References 19 publications

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“…However, in another study (Sharma and Rhyu 2014), C. lentillifera exhibited antidiabetic potential through different mechanism; via inhibition of dipeptidyl peptidase-IV and α-glucosidase in vitro.…”

Section: Discussionmentioning

confidence: 94%

Alpha-amylase, antioxidant, and anti-inflammatory activities of Eucheuma denticulatum (N.L. Burman) F.S. Collins and Hervey

et al. 2015

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Eucheuma denticulatum is a red edible seaweed that grows in the East Malaysia coastal region. The objective of this study was to investigate the α-amylase, antioxidant and anti-inflammatory activities of E. denticulatum ethanol extract and its three fractions (n-hexane, ethyl acetate and acetone). α-Amylase activity was assessed by dinitrosalicylic acid (DNS) assay, while the antioxidant property was determined by oxygen radical absorbance capacity (ORAC) analysis. The anti-inflammatory effects of the seaweed samples were evaluated by nitric oxide (NO), interleukin-6 (IL-6), interleukin-1 (IL-1), tumor necrosis factor-alpha (TNF-α), and monocyte chemoattractant protein-1 (MCP-1) activities on the interferon-gamma/lipopolysaccharide (IFN-γ/LPS)-stimulated murine macrophage cell line (RAW 264.7) using Griess reaction and immunoassays, respectively. At 10 mg mL −1 , E. denticulatum ethanol extract and three fractions inhibited α-amylase activities at variable levels. The highest (67 %) inhibition of α-amylase enzyme was by the ethanol crude extract. The three fractions showed inhibition with a mean of 42 %. Crude ethanol extracts also exhibited higher antioxidant capacity (36,400 ±23.5 mol Trolox equivalent (TE) (100 g) −1 ) when compared to the fractions. Crude extract and fractions (1-100 μg mL −1 ), also exhibited anti-inflammatory activity without showing any cytotoxic effect to RAW 264.7 cells. The present study suggests that E. denticulatum has the potential to be a promising source of effective functional metabolite. An extensive research on the edible varieties would contribute to a better understanding of their importance as functional food.

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Section: Discussionmentioning

confidence: 94%

Alpha-amylase, antioxidant, and anti-inflammatory activities of Eucheuma denticulatum (N.L. Burman) F.S. Collins and Hervey

et al. 2015

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“…After 1hr incubation, the reaction was stopped by the addition of 70 μl of 3M acetic acid. Then, released AMC was measured using a fluorescence microplate reader, Perkin Elmer Victor3 V 1420 multilabel plate counter (Perkin Elmer, USA) at an excitation wavelength of 370 nm and an emission wavelength for 440 nm (Sharma & Rhyu, ). For protein tyrosine phosphatase (PTP)‐1B assay, TCSE (3 μl in DMSO) was added to a reaction mixture containing enzyme (2 μl), reaction buffer (10 μl, 50mM citrate, pH 6.0, 0.1M NaCl, 1mM EDTA, and 1mM dithiothreitol), water (35 μl), and 50 μl of p ‐nitrophenylphosphate (20 mM).…”

Section: Methodsmentioning

confidence: 99%

“…Insulin binds to its receptor in metabolic tissues such as adipocytes and myocytes and upregulates the expression of glucose transporter (GLUT)‐4 that translocate to the plasma membrane and enhances the uptake of glucose inside the cells (Shimoda, Okada, Yamada, Pessin, & Yamada, ). Several studies highlighted that drugs that regulate both the insulin secretion and insulin action would be ideal antidiabetic drugs (B. R. Sharma & Rhyu, ). However, most of the drugs available so far target one of these pivotal processes of glucose metabolism and are associated with side effects on their long‐term uses (American Diabetes, ).…”

Section: Introductionmentioning

confidence: 99%

Tinospora cordifolia preserves pancreatic beta cells and enhances glucose uptake in adipocytes to regulate glucose metabolism in diabetic rats

Sharma

Park

Kim

et al. 2019

Phytotherapy Research

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The purpose of this study was to evaluate the pancreatic beta cell protective and glucose uptake enhancing effect of the water extract of Tinospora cordifolia stem (TCSE) by using rat insulinoma (RIN)-m5F cells and 3 T3-L1 adipocytes. RIN-m5F cells were stimulated with interleukin-1β and interferon-γ, and the effect of TCSE on insulin secretion and cytokine-induced toxicity was measured by ELISA and MTT assay, respectively. The glucose uptake and protein expression were measured by fluorometry and western blotting. Antidiabetic effect of TCSE was measured using streptozotocin-induced diabetic rats. TCSE dose dependently increased cell viability and insulin secretion in RIN-m5F cells. In addition, TCSE increased both the glucose uptake and glucose transporter 4 translocation in 3 T3-L1 adipocytes via PI3K pathway.Finally, TCSE significantly lowered blood glucose and diet intake and increased body weight in streptozotocin-induced diabetic rats. The level of serum insulin and hepatic glycogen was increased, whereas the level of serum triglyceride, total cholesterol, dipeptidyl peptidase-4, and thiobarbituric acid reactive substances was decreased in TCSE-administered rats. TCSE also increased glucose transporter 4 protein expression in the adipose tissue and liver of TCSE-fed diabetic rats. Our results suggested that TCSE preserved RIN-m5F cells from cytokine-induced toxicity and enhanced glucose uptake in 3 T3-L1 adipocytes, which may regulate glucose metabolism in diabetic rats.

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“…Results showed that C. lentillifera extract significantly decreased DPP-IV and α-glucosidase activities and, therefore, C. lentillifera could be used as a potential anti-diabetic agent [25].…”

Section: Dpp-iv Inhibitory Activitymentioning

confidence: 98%

In VITRO ANTIDIABETIC EFFECTS OF FERULA ASSA-FOETIDA EXTRACTS THROUGH DIPEPTIDYL PEPTIDASE IV AND Α-Glucosidase INHIBITORY ACTIVITY

Adel

Kumle

Niazi‎

2017

Asian J Pharm Clin Res

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Objective: Diabetes mellitus (DM) causes hyperglycemia, which is one of the most common diseases in the world. One of the strategies for the treatment of diabetes is maintaining postprandial glucose level through inhibition of dipeptidyl peptidase IV (DPP-IV) and α-glucosidase enzymes. The aim of this study was to determine in vitro antidiabetic potential of Ferula assa-foetida via DPP-IV and α-glucosidase inhibitory activities.Methods: F. assa-foetida seeds were extracted in methanol, ethanol, ethanol-methanol, and water. Inhibitory activity on DPP-IV and α-glucosidase was performed in vitro and measured spectrophotometrically at λ=405 nm. Results:The result showed that the F. assa-foetida seed extract is effective against both enzymes. All fractions had DPP-IV inhibitory activity, but the ethanolic fraction had the highest inhibitory activity on DPP-IV enzyme and significantly decreased DPP-IV activity (24.5%). With respect to α-glucosidase inhibitory activity, the aqueous extract has the highest inhibitory activity (28%). Conclusion:According to the results of this study, F. assa-foetida contains DPP-IV and α-glucosidase inhibitors and could be a potential source for the discovery of active constituents as α-glucosidase and DPP-IV inhibitors to treat Type 2 DM.

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Anti-diabetic effects of Caulerpa lentillifera: stimulation of insulin secretion in pancreatic β-cells and enhancement of glucose uptake in adipocytes

Abstract: Thus, our results suggest that C. lentillifera could be used as a potential anti-diabetic agent.

Cited by 71 publications

References 19 publications

Alpha-amylase, antioxidant, and anti-inflammatory activities of Eucheuma denticulatum (N.L. Burman) F.S. Collins and Hervey

Alpha-amylase, antioxidant, and anti-inflammatory activities of Eucheuma denticulatum (N.L. Burman) F.S. Collins and Hervey

Tinospora cordifolia preserves pancreatic beta cells and enhances glucose uptake in adipocytes to regulate glucose metabolism in diabetic rats

In VITRO ANTIDIABETIC EFFECTS OF FERULA ASSA-FOETIDA EXTRACTS THROUGH DIPEPTIDYL PEPTIDASE IV AND Α-Glucosidase INHIBITORY ACTIVITY

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