The pharmacognostic standardization of natural products is a crucial stage in describing the product's quality. The present investigation presents pharmacognostic standards of dried roots of Cyperus rotundus. Utilizing standardized techniques, a number of pharmacognostic standards were produced, including ash values, extractive values, moisture content, foreign organic matter, swelling index, foaming index, tannin determination, and volatile oil content. Major bioactive components, including flavonoids, phenolic compounds, tannins, alkaloids, and triterpenoids, were detected in plant extracts using phytochemical screening. Remarkably, coumarin was detected in the methanol extract.Its existence was confirmed by measuring the total phenol and total flavonoids content. Using an optimized solvent system, the fingerprint profile of ethyl acetate and methanol extracts revealed four and two spots, respectively, in thin layer chromatography. To conclude, the selected plant was perfect and suitable for further in vitro as well as in vivo studies to introduce a good herbal product in herbal drug industry.